Methods in vitamin B-6 nutrition : analysis and status assessment

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Bibliographic Information

Methods in vitamin B-6 nutrition : analysis and status assessment

edited by James E. Leklem and Robert D. Reynolds

Plenum Press, c1981

  • : softcover

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"Softcover reprint of the hardcover 1st edition 1981"--T.p. verso

Proceedings of the workshop held June 29-July 2, 1980

Includes bibliographies and index

Description and Table of Contents

Description

During the period 29 June to 2 July 1980, a Workshop was held at Timberline Lodge, Mt. Hood, Oregon, entitled "Vitamin B-6 Analyt ical Methodology and Criteria for Assessing Nutritional Status". The papers which follow are the proceedings of that Workshop and represent the combined works of 36 scientists from across the United States and Canada. In addition to the 19 papers, there were seven short presentations which we consider to be at the very frontiers of analytical methods and status assessment for vitamin B-6. These reports discussed such areas as production of specific antibodies to the various vitamers, the synthesis of deuterated vitamers, comparisons of ultraviolet, fluorescence and elctrochemical deter minations of the vitamers and the identification of polyamine pyridoxal Schiff bases in urine. Those reports are not included in this book solely due to their highly tentative and preliminary findings, not because we considered them of any less importance than the papers presented herein. In fact, these reports may well provide the foundations upon which subsequent workshops are devel oped. The feeling for the need of such a Workshop in part grew out of a previous workshop held at the Letterman Army Institute of Research, San Fra"ncisco, California, 11-12 June 1976.

Table of Contents

Methodology of Vitamin B-6 Analyses.- Vitamin B-6 Analysis: Some Historical Aspects.- Microbiological Assay of Vitamin B-6 in Foods.- Microbiological Assay of Vitamin B-6 in Blood and Urine.- Microassay of Pyridoxal Phosphate Using Tyrosine Apodecarboxylase.- An Improved Colorimetric Assay for Pyridoxal Phosphate Using Highly Purified Apotryptophanase.- Simple Assay for Femtomoles of Pyridoxal and Pyridoxamine Phosphates.- Chemical Analysis of Pyridoxine Vitamers.- Extraction and Quantitation of B-6 Vitamins from Animal Tissues and Human Plasma: A Preliminary Study.- Determination of Vitamin B-6 Compounds by Semiautomated Continuous-Flow and Chromatographic Methods.- Methodology for Determination of Blood Aminotransferases.- Criteria for Vitamin B-6 Status Assessment.- Vitamin B-6 Status Assessment: Past and Present.- Estimated Dietary Intakes of Vitamin B-6.- Assessment of Vitamin B-6 Status in Infants and Children: Serial Pyridoxal Phosphate Levels in Premature Infants.- Vitamin B-6 Content in Human Milk.- Plasma PLP as Indicator of Nutrition Status: Relationship to Tissue Vitamin B-6 Content and Hepatic Metabolism.- Urinary 4-Pyridoxic Acid, Urinary Vitamin B-6 and Plasma Pyridoxal Phosphate as Measures of Vitamin B-6 Status and Dietary Intake of Adults.- The Tryptophan Load Test as an Index of Vitamin B-6 Nutrition.- Vitamin B-6 and Sulfur Amino Acid Metabolism.- Methionine Metabolite Excretion as Affected by a Vitamin B-6 Deficiency.- Evaluation and Recommendations.- Recommended Methods for Vitamin B-6 Analysis.- Recommendations for Assessment of Vitamin B-6 Status.- List of Participants.

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