Lipid rafts : methods and protocols

Author(s)

• Bieberich, Erhard

Bibliographic Information

Lipid rafts : methods and protocols

edited by Erhard Bieberich

（Methods in molecular biology / John M. Walker, series editor, 2187）（Springer protocols）

Humana Press, c2021

Note

Includes bibliographical references and index

Description and Table of Contents

Description

This volume gives a comprehensive insight into established and novel methods to analyze the structure and function of lipid rafts. This book covers topics such as isolation of lipid rafts and their functional analysis using biochemical methods; visualization of lipid rafts and their interaction with proteins using fluorescence-related methods; preparation of giant lipid vesicles and fluorescence spectroscopy; FRET and FRAP; and using photo-activated cross-linking of a ceramide analog combined with proximity ligation assay. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Lipid Rafts: Methods and Protocols is a valuable resource for both novice and expert researchers interested in learning more about the function of lipid rafts in many areas of cell biology and medicine.

Table of Contents

Foreword... Preface... Table of Contents... Contributing Authors... 1. Isolation and Analysis of Lipid Rafts from Neural Cells and Tissues Sara Grassi, Paola Giussani, Laura Mauri, Simona Prioni, and Alessandro Prinetti 2. A Detergent-Free Method for Preparation of Lipid Rafts for the Shotgun Lipidomics Study Chao Qin, Meixia Pan, and Xianlin Han 3. Biochemical Analysis of Lipid Rafts to Study Pathogenic Mechanisms of Neural Diseases Ernesto R. Bongarzone and Maria Irene Givogri 4. Amyloid- Interactions with Lipid Rafts in Biomimetic Systems: A Review of Laboratory Methods Galya Staneva, Chiho Watanabe, Nicolas Puff, Vesela Yordanova, Michel Seigneuret, and Miglena Angelova 5. Extracellular Vesicles Containing Ceramide-Rich Platforms: "Mobile Raft" Isolation and Analysis Ahmed Elsherbini, Haiyan Qin, Zhihui Zhu, Priyanka Tripathi, Guanghu Wang, Simone M. Crivelli, Stefanka D. Spassieva, and Erhard Bieberich 6. Isolation of Lipid Rafts (Detergent-Resistant Microdomains) and Comparison to Extracellular Vesicles (Exosomes) Glyn Dawson 7. Methods to Characterize Synthesis and Degradation of Sphingomyelin at the Plasma Membrane and Its Impact on Lipid Raft Dynamics Mariana Nikolova-Karakashian 8. Association of Glycolipids and Growth Factor Receptors in Lipid Rafts Jing Wang and Robert K. Yu 9. Lipid Raft Isolation by Sucrose Gradient Centrifugation and Visualization of Raft-Located Proteins by Fluorescence Microscopy: The Use of Combined Techniques to Assess Fas/CD95 Location in Rafts during Apoptosis Triggering Consuelo Gajate and Faustino Mollinedo 10. Elucidating the Role of Lipid Rafts on G Protein-Coupled Receptor Function in the Mouse Kidney: An In Vivo Approach Laureano D. Asico, Selim Rozyyev, Annabelle M. Crusan, Pedro A. Jose, and Van Anthony M. Villar 11. Analysis of Lipids in Ceramide-Enriched Membrane Domains Alexander Carpinteiro and Erich Gulbins 12. Chemotherapeutic Agents-Induced Ceramide Rich Platforms (CRPs) in Endothelial Cells and their Modulation Prerna R. Nepali and Adriana Haimovitz-Friedman 13. Biophysical Analysis of Lipid Domains by Fluorescence Microscopy Ana E. Ventura, Tania C. B. Santos, Joaquim T. Marques, Rodrigo F.M. de Almeida, and Liana C. Silva 14. Biophysical Analysis of Lipid Domains in Mammalian and Yeast Membranes by Fluorescence Spectroscopy Carla Sousa, Filipa C. Santos, Andreia Bento-Oliveira, Beatriz Mestre, Liana C. Silva, and Rodrigo F. M. de Almeida 15. Characterization of Lipid Order and Domain Formation in Model Membranes Using Fluorescence Microscopy and Spectroscopy Andrew Fuhrer and Amir M. Farnoud 16. Characterization of the Effect of Sphingolipid Accumulation on Membrane Compactness, Dipole Potential, and Mobility of Membrane Components Gyula Batta, Timea Hajdu, and Peter Nagy 17. Super-Resolution Microscopy of Sphingolipids Jan Schlegel and Markus Sauer 18. Detection of Functionalized Sphingolipid Analogues in Detergent Resistant Membranes of the Immune Cells Julian Fink, Jurgen Seibel, and Elita Avota 19. Immunofluorescence Labeling of Lipid-Binding Proteins CERTs to Monitor Lipid Rafts Dynamics Caterina Giovagnoni, Simone M. Crivelli, Mario Losen, and Pilar Martinez-Martinez 20. Cross-Link/Proximity Ligation Assay for Visualization of Lipid and Protein Complexes in Lipid Rafts Priyanka Tripathi, Zhihui Zhu, Haiyan Qin, Ahmed Elsherbini, Emily A. Roush, Simone M. Crivelli, Stefanka D. Spassieva, and Erhard Bieberich Subject Index List...

by "Nielsen BookData"

• NCID: BC02927608
• ISBN:
  • 9781071608135
• Country Code: us
• Title Language Code: eng
• Text Language Code: eng
• Place of Publication: New York
• Pages/Volumes: xi, 350 p.
• Size: 27 cm
• Classification:
  • LCC : QP751
  • DC23 : 572/.57
• Subject Headings:
  • LCSH : Lipids -- Laboratory manuals
• Parent Bibliography ID:
  • BA10794673
  • BA88873632