Plant cold acclimation : methods and protocols

書誌事項

Plant cold acclimation : methods and protocols

edited by Dirk K. Hincha and Ellen Zuther

(Springer protocols)(Methods in molecular biology / John M. Walker, series editor, 2156)

Humana Press, c2020

2nd ed

  • : [hardback]

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

This detailed new edition combines a wide selection of experimental methods for plant cold acclimation, deacclimation, and cold memory, ranging from the whole plant level of ecology and breeding to molecular profiling and the detailed analysis of specific proteins, with many levels of investigated complexity in between. The collection updates methodology from the first edition while adding chapters on numerous key advances in the field. Written for the highly successful Method in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Plant Cold Acclimation: Methods and Protocols, Second Edition seeks to aid researchers, both new and experienced, in entering this exciting field of research or broadening the scope of their investigations.

目次

1. Introduction: Plant Cold Acclimation and Winter Survival Dirk K. Hincha and Ellen Zuther 2. Measuring Freezing Tolerance of Leaves and Rosettes: Electrolyte Leakage and Chlorophyll Fluorescence Assays Anja Thalhammer, Majken Pagter, Dirk K. Hincha, and Ellen Zuther 3. Differential Thermal Analysis: A Fast Alternative to Frost Tolerance Measurements Andrey V. Malyshev, Ilka Beil, and Juergen Kreyling 4. Infrared Thermal Analysis of Plant Freezing Processes Gilbert Neuner and Edith Kuprian 5. Conducting Field Trials for Frost Tolerance Breeding in Cereals Luigi Cattivelli and Cristina Crosatti 6. A Whole-Plant Screening Test to Select Freezing-Tolerant and Low-Dormant Genotypes Annick Bertrand, Annie Claessens, Josee Bourassa, Solen Rocher, and Vern Baron 7. Mapping of Quantitative Trait Loci (QTL) Associated with Plant Freezing Tolerance and Cold Acclimation Evelyne Teoule and Carine Gery 8. Identification of Arabidopsis Mutants with Altered Freezing Tolerance Carlos Perea-Resa, Rafael Catala, and Julio Salinas 9. Cryo-Scanning Electron Microscopy to Study the Freezing Behavior of Plant Tissues Seizo Fujikawa and Keita Endoh 10. Using Pixel-Based Microscope Images to Generate 3-D Reconstructions of Frozen and Thawed Plant Tissue David P. Livingston III and Tan D. Tuong 11. Phenotyping Plant Cellular and Tissue Level Responses to Cold with Synchrotron-Based Fourier-Transform Infrared Spectroscopy and X-Ray Computed Tomography Ian R. Willick, Jarvis Stobbs, Chithra Karunakaran, and Karen K. Tanino 12. Proteomic Approaches to Identify Proteins Responsive to Cold Stress Anna M. Jozefowicz, Stefanie Doell, and Hans-Peter Mock 13. Proteomic Approaches to Identify Cold Regulated Plasma Membrane Proteins Kamal Md. Mostafa, Daisuke Takahashi, Takato Nakayama, Yushi Miki, Yukio Kawamura, and Matsuo Uemura 14. A Lipidomic Approach to Identify Cold-Induced Changes in Arabidopsis Membrane Lipid Composition Yu Song, Hieu Sy Vu, Sunitha Shiva, Carl Fruehan, Mary R. Roth, Pamela Tamura, and Ruth Welti 15. Multiplexed Profiling and Data Processing Methods to Identify Temperature-Regulated Primary Metabolites Using Gas Chromatography Coupled to Mass Spectrometry Alexander Erban, Federico Martinez-Seidel, Yogeswari Rajarathinam, Frederik Dethloff, Isabel Orf, Ines Fehrle, Jessica Alpers, Olga Beine-Golovchuk, and Joachim Kopka 16. Determining the ROS and the Antioxidant Status of Leaves during Cold Acclimation Andras Bittner, Thomas Griebel, Joern van Buer, Ilona Juszczak-Debosz, and Margarete Baier 17. Analysis of Changes in Plant Cell Wall Composition and Structure during Cold Acclimation Daisuke Takahashi, Ellen Zuther, and Dirk K. Hincha 18. Subcellular Compartmentation of Metabolites Involved in Cold Acclimation Imke I. Hoermiller, Thomas Nagele, and Arnd G. Heyer 19. Mathematical Modeling of Plant Metabolism in a Changing Temperature Regime Lisa Furtauer and Thomas Nagele 20. Characterization of Ice Binding Proteins from Sea-Ice Microalgae Maddalena Bayer-Giraldi, EonSeon Jin, and Peter W. Wilson 21. Isolation and Characterization of Ice-Binding Proteins from Higher Plants Melissa Bredow, Heather E. Tomalty, Laurie A. Graham, Audrey K. Gruneberg, Adam J. Middleton, Barbara Vanderbeld, Peter L. Davies, and Virginia K. Walker

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