Plant cold acclimation : methods and protocols
Author(s)
Bibliographic Information
Plant cold acclimation : methods and protocols
(Springer protocols)(Methods in molecular biology / John M. Walker, series editor, 2156)
Humana Press, c2020
2nd ed
- : [hardback]
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Note
Includes bibliographical references and index
Description and Table of Contents
Description
This detailed new edition combines a wide selection of experimental methods for plant cold acclimation, deacclimation, and cold memory, ranging from the whole plant level of ecology and breeding to molecular profiling and the detailed analysis of specific proteins, with many levels of investigated complexity in between. The collection updates methodology from the first edition while adding chapters on numerous key advances in the field. Written for the highly successful Method in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Plant Cold Acclimation: Methods and Protocols, Second Edition seeks to aid researchers, both new and experienced, in entering this exciting field of research or broadening the scope of their investigations.
Table of Contents
1. Introduction: Plant Cold Acclimation and Winter Survival
Dirk K. Hincha and Ellen Zuther
2. Measuring Freezing Tolerance of Leaves and Rosettes: Electrolyte Leakage and Chlorophyll Fluorescence Assays
Anja Thalhammer, Majken Pagter, Dirk K. Hincha, and Ellen Zuther
3. Differential Thermal Analysis: A Fast Alternative to Frost Tolerance Measurements
Andrey V. Malyshev, Ilka Beil, and Juergen Kreyling
4. Infrared Thermal Analysis of Plant Freezing Processes
Gilbert Neuner and Edith Kuprian
5. Conducting Field Trials for Frost Tolerance Breeding in Cereals
Luigi Cattivelli and Cristina Crosatti
6. A Whole-Plant Screening Test to Select Freezing-Tolerant and Low-Dormant Genotypes
Annick Bertrand, Annie Claessens, Josee Bourassa, Solen Rocher, and Vern Baron
7. Mapping of Quantitative Trait Loci (QTL) Associated with Plant Freezing Tolerance and Cold Acclimation
Evelyne Teoule and Carine Gery
8. Identification of Arabidopsis Mutants with Altered Freezing Tolerance
Carlos Perea-Resa, Rafael Catala, and Julio Salinas
9. Cryo-Scanning Electron Microscopy to Study the Freezing Behavior of Plant Tissues
Seizo Fujikawa and Keita Endoh
10. Using Pixel-Based Microscope Images to Generate 3-D Reconstructions of Frozen and Thawed Plant Tissue
David P. Livingston III and Tan D. Tuong
11. Phenotyping Plant Cellular and Tissue Level Responses to Cold with Synchrotron-Based Fourier-Transform Infrared Spectroscopy and X-Ray Computed Tomography
Ian R. Willick, Jarvis Stobbs, Chithra Karunakaran, and Karen K. Tanino
12. Proteomic Approaches to Identify Proteins Responsive to Cold Stress
Anna M. Jozefowicz, Stefanie Doell, and Hans-Peter Mock
13. Proteomic Approaches to Identify Cold Regulated Plasma Membrane Proteins
Kamal Md. Mostafa, Daisuke Takahashi, Takato Nakayama, Yushi Miki, Yukio Kawamura, and Matsuo Uemura
14. A Lipidomic Approach to Identify Cold-Induced Changes in Arabidopsis Membrane Lipid Composition
Yu Song, Hieu Sy Vu, Sunitha Shiva, Carl Fruehan, Mary R. Roth, Pamela Tamura, and Ruth Welti
15. Multiplexed Profiling and Data Processing Methods to Identify Temperature-Regulated Primary Metabolites Using Gas Chromatography Coupled to Mass Spectrometry
Alexander Erban, Federico Martinez-Seidel, Yogeswari Rajarathinam, Frederik Dethloff, Isabel Orf, Ines Fehrle, Jessica Alpers, Olga Beine-Golovchuk, and Joachim Kopka
16. Determining the ROS and the Antioxidant Status of Leaves during Cold Acclimation
Andras Bittner, Thomas Griebel, Joern van Buer, Ilona Juszczak-Debosz, and Margarete Baier
17. Analysis of Changes in Plant Cell Wall Composition and Structure during Cold Acclimation
Daisuke Takahashi, Ellen Zuther, and Dirk K. Hincha
18. Subcellular Compartmentation of Metabolites Involved in Cold Acclimation
Imke I. Hoermiller, Thomas Nagele, and Arnd G. Heyer
19. Mathematical Modeling of Plant Metabolism in a Changing Temperature Regime
Lisa Furtauer and Thomas Nagele
20. Characterization of Ice Binding Proteins from Sea-Ice Microalgae
Maddalena Bayer-Giraldi, EonSeon Jin, and Peter W. Wilson
21. Isolation and Characterization of Ice-Binding Proteins from Higher Plants
Melissa Bredow, Heather E. Tomalty, Laurie A. Graham, Audrey K. Gruneberg, Adam J. Middleton, Barbara Vanderbeld, Peter L. Davies, and Virginia K. Walker
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