Arbuscular mycorrhizal fungi : methods and protocols
Author(s)
Bibliographic Information
Arbuscular mycorrhizal fungi : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 2146）（Springer protocols）
Humana Press, c2020
- : [hardback]
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Note
Includes bibliographical references and index
Description and Table of Contents
Description
This book compiles the most comprehensive collection of protocols currently used in arbuscular mycorrhizal (AM) fungal research. This experience-based collection includes methods for isolation, cultivation, detection, and quantification of AM fungi, as well as the use of metagenomics for community studies and experimental procedures for functional genomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Arbuscular Mycorrhizal Fungi: Methods and Protocols serves as an ideal aid for researchers seeking to perform experiments that fill the gaps in our knowledge of the basic biology, functions, and ecology of AM fungi in the hope of deploying these powerful plant fungal symbionts in agriculture more effectively.
Table of Contents
1. Isolation and Culture of Arbuscular Mycorrhizal Fungi from Field Samples
Dora Trejo-Aguilar and Jacob Banuelos
2. The Mycorrhizal Donor Plant (MDP) In Vitro Culture System for the Efficient Colonization of Whole Plants
Ismahen Lalaymia and Stephane Declerck
3. A Whole-Plant Culture Method to Study Structural and Functional Traits of Extraradical Mycelium
Cristiana Sbrana, Alessandra Pepe, Nuria Ferrol, and Manuela Giovannetti
4. Histochemical Staining and Quantification of Arbuscular Mycorrhizal Fungal Colonization
Tania Ho-Plagaro, Maria Isabel Tamayo-Navarrete, and Jose Manuel Garcia-Garrido
5. Fluorescent Staining of Arbuscular Mycorrhizal Structures Using Wheat Germ Agglutinin (WGA) and Propidium Iodide
Gennaro Carotenuto and Andrea Genre
6. Visualization of Arbuscular Mycorrhizal Fungal Extra-Radical Hyphae and Spores Vitality and Activity
Pablo Cornejo and Humberto Aponte
7. Molecular Quantification of Arbuscular Mycorrhizal Fungal Root Colonization
Martina Janouskova and Petra Caklova
8. Single-Spore-Extraction for Genetic Analyses of Arbuscular Mycorrhizal Fungi
Dirk Redecker
9. High Throughput DNA Sequence-Based Analysis of AMF Communities
Iris Marisa Maxaieie Victorino, Andrea Berruti, Alberto Orgiazzi, Samuele Voyron, Valeria Bianciotto, and Erica Lumini
10. Analysis of Arbuscular Mycorrhizal Fungal Communities by Terminal Restriction Fragment Length Polymorphism (TRFLP)
Alvaro Lopez-Garcia
11. Bioinformatic Methods for the Analysis of High-Throughput RNA Sequencing in Arbuscular Mycorrhizal Fungi
Francesco Venice, Alessandra Salvioli di Fossalunga, and Paola Bonfante
12. Arbuscular Mycorrhizal Fungal Gene Expression Analysis by Real-Time PCR
Alessandro Silvestri, Jacob Perez-Tienda, and Juan Antonio Lopez-Raez
13. Laser Microdissection as a Useful Tool to Study Gene Expression in Plant and Fungal Partners in AM Symbiosis
Raffaella Balestrini and Valentina Fiorilli
14. Detection of Arbuscular Mycorrhizal Fungal Gene Expression by In Situ Hybridization
Bettina Hause and Natalia Requena
15. Functional Analyses of Arbuscular Mycorrhizal Fungal Genes in Yeast
Elisabeth Tamayo, Tamara Gomez-Gallego, and Nuria Ferrol
16. Isotope Labelling to Study Phosphorus Uptake in the Arbuscular Mycorrhizal Symbiosis
Carla Cruz-Paredes and Mayra E. Gavito
17. Recovery of Extra-Radical Fungal Peptides Amenable for Shotgun Protein Profiling in Arbuscular Mycorrhizae
Ghislaine Recorbet, Pierre-Emmanuel Courty, and Daniel Wipf
18. Host-Induced Gene Silencing of Arbuscular Mycorrhizal Fungal Genes via Agrobacterium Rhizogenes-Mediated Root Transformation in Medicago truncatula
Meike Hartmann, Stefanie Voss, and Natalia Requena
19. Application of Virus-Induced Gene Silencing to Arbuscular Mycorrhizal Fungi
Tatsuhiro Ezawa, Hayato Maruyama, Yusuke Kikuchi, Kaede Yokoyama, and Chikara Masuta
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