Microglia : methods and protocols
著者
書誌事項
Microglia : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 2034）
Humana Press, c2019
大学図書館所蔵 全1件
内容説明・目次
内容説明
This book presents a comprehensive toolkit of versatile techniques for studying microglia under different experimental settings along with a brief summary of knowledge, accumulated in microglial research over the last decades. Beginning with recently discovered roles of microglia in health and disease, the volume continues by covering in vitro analyses of microglia, in vivo studies, and "omics" analyses. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Microglia: Methods and Protocols serves as a vital guide to these important cells and an inspiration for scientists interested in expanding our knowledge of their role in the nervous system.
目次
Part I: Microglia in Health and Disease
1. Microglia: The Neural Cells of Non-Neural Origin
Olga Garaschuk and Alexei Verkhratsky
2. Morphology of Microglia across Contexts of Health and Disease
Julie C. Savage, Micael Carrier, and Marie-Eve Tremblay
3. Physiology of Microglia
Olga Garaschuk and Alexei Verkhratsky
4. Microglia Reactivity: Heterogeneous Pathological Phenotypes
Helene Hirbec, Francois Rassendren, and Etienne Audinat
5. Microglia in Neurodegenerative Disorders
Dario Tejera and Michael T. Heneka
6. Physiological Implications of Microglia-Synapse Interactions
Hiroaki Wake, Hiroshi Horiuchi, Daisuke Kato, Andrew J. Moorhouse, and Junichi Nabekura
Part II: In Vitro Analyses of Microglia
7. Ultrastructural Analyses of Microglial Interactions with Synapses
Marie-Eve Tremblay and Ania K. Majewska
8. Visualizing Dark Microglia
Marie-Kim St-Pierre, Maude Bordeleau, and Marie-Eve Tremblay
9. Electrophysiological Investigation of Microglia
Elena Avignone, Giampaolo Milior, Isabelle Arnoux, and Etienne Audinat
10. Replenishment of Organotypic Hippocampal Slice Cultures with Neonatal or Adult Microglia
Annette Masuch and Knut Biber
11. Assessment of Parasite-Microglia Interactions In Vitro
Katherine Figarella, Nestor L. Uzcategui, and Michael Duszenko
Part III: Studying Microglia In Vivo
12. In Vivo Visualization of Microglia Using Tomato Lectin
Bianca Brawek, Maria Olmedillas del Moral, and Olga Garaschuk
13. Long-Term In Vivo Imaging of Individual Microglial Cells
Angelos A. Skodras, Jasmin Hefendehl, and Jonas J. Neher
14. Analysis of Signaling Mechanisms Regulating Microglial Process Movement
Vasiliki Kyrargyri, David Attwell, Renaud Blaise Jolivet, and Christian Madry
15. Measuring Microglial Turnover in the Adult Brain
Diego Gomez-Nicola, Gemma L. Fryatt, and Katharine E. Askew
16. Novel Microglia Depletion Systems: A Genetic Approach Utilizing Conditional Diphtheria Toxin Receptor Expression and a Pharmacological Model Based on the Blocking of Macrophage Colony-Stimulating Factor 1 Receptor
Maja Kitic, Peter See, Julia Bruttger, Florent Ginhoux, and Ari Waisman
17. Single Cell Electroporation for Measuring In Vivo Calcium Dynamics in Microglia
Bianca Brawek and Olga Garaschuk
18. Labeling Microglia with Genetically-Encoded Calcium Indicators
Yajie Liang and Olga Garaschuk
19. Calcium Imaging of Microglial Network Activity in Stroke
Petr Tvrdik, Kathryn N. Kearns, Khadijeh A. Sharifi, M. Filip Sluzewski, Scott T. Acton, and M. Yashar S. Kalani
20. In Vivo Two Photon Imaging of Microglial Synapse Contacts
Daisuke Kato, Ako Ikegami, Hiroshi Horiuchi, Andrew J. Moorhouse, Junichi Nabekura, and Hiroaki Wake
21. In Vivo Phagocytosis Analysis of Amyloid Beta
Dario Tejera and Michael T. Heneka
22. Functional Analysis of Brain-Engrafted Monocytes after Microglial Ablation in Mouse Models
Nicholas H. Varvel, Richard M. Ransohoff, and Jonas J. Neher
Part IV: Omics Analyses of Microglia
23. Analysis of the Microglial Sensome
Suzanne E. Hickman and Joseph El Khoury
24. Transcriptomics and Live Imaging to Define Functional Phenotypes of Microglia in Pathological Human Tissue
Giampaolo Milior, Farah Chali, Tiffany Dos Santos, Juliette Royer, Richard Miles, and Melanie Morin-Brureau
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