CiNii 図書 - In situ hybridization protocols

This fifth edition volume expands on the previous editions with updated discussions on the many new in situ hybridization (ISH) techniques used by researchers today. New developments in probe designs, detection systems, specificity and sensitivity improvements, and multiplexing combinations are explored. Chapters in this book are organized into seven sections and cover general applications; methods for DNA ISH; methods for cultured cells; methods for wholemount and plant material; automated methods for RNA; multiplexing and combined methods; and targeted selective methods and single molecule detections. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, In Situ Hybridization Protocols, Fifth Edition is a valuable resource for both novice and expert scientists interested in learning more about this exciting and advancing field.

Preface... Table of Contents... Contributing Authors... Part I General Applications 1. Guidelines for the Optimization and Validation of In Situ Hybridization William J. Howat and Julia Jones 2. Overcoming Autofluorescence (AF) and Tissue Variation in Image Analysis of In Situ Hybridization Cara Brodie Part II Methods for DNA ISH 3. Practical Application of Fluorescent In Situ Hybridization Techniques in Clinical Diagnostic Laboratories Sheila J.M. O'Connor, Kathryn R. Turner, and Sharon L. Barrans 4. Fluorescent In Situ Hybridization using Oligonucleotide-Based Probes Guilherme T. Braz, Fan Yu, Livia do Vale Martins, and Jiming Jiang 5. Visualizing Genome Reorganization using 3D DNA FISH Alasdair Jubb and Shelagh Boyle Part III Methods for Cultured Cells 6. MicroRNA In Situ Hybridization in Paraffin Embedded Cultured Cells Jaslin P. James, Laura Johnsen, Trine Moller, and Boye S. Nielsen 7. Multiplexed Detection and Analysis of Low Abundant Abundance Long Non-Coding RNA using RNAscopeTM in Cultured Cells Julia Jones, Heather Zecchini, and Sankari Nagarajan 8. Multiplexed Quantitative In Situ Hybridization for Mammalian or Bacterial Cells in Suspension: qHCR Flow Cytometry (v3.0) Maayan Schwarzkopf, Harry M.T. Choi, and Niles A. Pierce 9. Multiplexed Quantitative In Situ Hybridization for Mammalian Cells on a Slide: qHCR and dHCR Imaging (v3.0) Maayan Schwarzkopf, Harry M.T. Choi, and Niles A. Pierce Part IV Methods for Wholemounts and Plant Material 10. Multiplexed Quantitative In Situ Hybridization with Subcellular or Single-Molecule Resolution within Whole-Mount Vertebrate Embryos: qHCR and dHCR imaging (v3.0) Harry M.T. Choi, Maayan Schwarzkopf, and Niles A. Pierce 11. Hybridization Chain Reaction for Quantitative and Multiplex Imaging of Gene Expression in Amphioxus Embryos and Adult Tissues Toby G.R. Andrews, Giacomo Gattoni, Lara Busby, Michael A. Schwimmer, and Elia Benito-Gutierrez 12. RNAscopeTM Multiplex Detection in Zebrafish Theresa Gross-Thebing 13. Duplex In Situ Hybridization of Virus Nucleic Acids in Plant Tissues using RNAscopeTM Samar Sheat, Stephan Winter, and Paolo Margaria Part V Automated Methods for RNA 14. Automated ISH for Validated Histological Mapping of Lowly Expressed Genes Charles Pyke 15. Automation of Multiplexed RNAscope Single-Molecule Fluorescent In Situ Hybridization and Immunohistochemistry for Spatial Tissue Mapping Kenny Roberts and Omer Ali Bayraktar 16. Automated Co-In Situ Hybridization and Immunofluorescence using Archival Tumor Tissue Leah K. Officer, Kleopatra E. Andreou, Ana V. Teodosio, Zhangyi He, and John P. Le Quesne 17. Automated 5-Color Multiplex Co-Detection of MicroRNA and Protein Expression in Fixed Tissue Specimens Lorenzo F. Sempere, Erin Zaluzec, Elizabeth Kenyon, Matti Kiupel, and Anna Moore 18. Mixed Multiplex Staining: Automated RNAscopeTM and OPALTM for Multiple Targets Michael Millar Part VI Multiplexing and Combined Methods 19. Simultaneous Visualization of RNA and Protein Expression in Tissue Using a Combined RNAscopeTM In Situ Hybridization and Immunofluorescence Protocol Anushka Dikshit, Hailing Zong, Courtney Anderson, Bingqing Zhang, and Xiao-Jun Ma 20. In Situ Sequencing: A High-Throughput, Multi-Targeted Gene Expression Profiling Technique for Cell Typing in Tissue Sections Markus M. Hilscher, Daniel Gyllborg, Chika Yokot, and Mats Nilsson 21. GeoMx (TM) RNA Assay: High Multiplex, Digital, Spatial Analysis of RNA in FFPE Tissue Daniel R. Zollinger, Stan E. Lingle, Kristina Sorg, Joseph M. Beechem, and Christopher R. Merritt Part VII Target Selective Methods and Single Molecule Detection 22. In Situ Point Mutation Detection in FFPE Colorectal Cancers using the BaseScope Assay Ann-Marie Baker and Trevor A. Graham 23. Using In Situ Padlock Probe Technology to Detect mRNA Splice Variants in Tumor Cells Lilli Hofmann, Thomas Kroneis, and Amin El-Heliebi 24. Automated One-Double-Z Pair BaseScope (TM) for CircRNA In Situ Hybridization Boye Schnack Nielsen, Trine Moller, and Jorgen Kjems

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In situ hybridization protocols

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