Aging : methods and protocols
著者
書誌事項
Aging : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 2144）（Springer protocols）
Humana Press, 2020
- : [hardback]
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注記
Description based on "Corrected publication 2020"
Includes bibliographical references and index
内容説明・目次
内容説明
This volume details the impact of aging on biological system and to understand the basis of this diversity with the goal of elucidating mechanisms to optimize health. Chapters guide readers through different animal models and methodologies to examine aging at the organism, tissue, cellular, and molecular levels across the lifespan. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Aging: Methods and Protocols aims to ensure successful results in the further study of this vital field.
目次
. Measuring the Replicative Lifespan of Saccharomyces cerevisiae using the HYAA Microfluidic Platform
Ruofan Yu, Myeong Chan Jo, and Weiwei Dang
2. Analysis of Lifespan in C. elegans: Low- and High-Throughput Approaches
Adam B. Cornwell and Andrew V. Samuelson
3. Simple Culture Apparatus for Individual C. elegans Culture
William E Pittman, Drew B Sinha, Holly E Kinser, Nisha S Patil, Eric S Terry, Isaac B Plutzer, Julia Hong, and Zachary Pincus
4. Analysis of Drosophila melanogaster Life Span
Gary N. Landis, Devon Doherty, and John Tower
5. Assessment of Mouse Fitness as Determined through Treadmill Running and Walking
Joseph C. Reynolds and Changhan Lee
6. Human Population Genetics in Aging Studies for Molecular Biologists
Brendan Miller, Amin Haghani, Jennifer Ailshire, and T. Em Arpawong
7. Design and Analysis of Pharmacological Studies in Aging
Khalyd J. Clay and Michael Petrascheck
8. Methods for Assessing Fertility in C. elegans from a Single Population
Chia-An Yen and Sean P. Curran
9. Metabolic Assessment of Lipid Abundance and Distribution
James D. Nhan and Sean P. Curran
10. Quantitative Profiling of Lipid Species in Caenorhabditis elegans with Gas Chromatography-Mass Spectrometry
Elizabeth C. Pino and Alexander A. Soukas
11. Assessing Insulin and Glucose Tolerance in the Aging Mouse
Roberta Buono and Sebastian Brandhorst
12. High-throughput Assessment of Changes in the Caenorhabditis elegans Gut Microbiome
Fan Zhang, Jessica L. Weckhorst, Adrien Assie, Anastasia S. Khodakova, Mario Loeza-Cabrera, Daniela Vidal, and Buck S. Samuel
13. Measurements of Innate Immune Function in C. elegans Kyle J. Foster, Deborah L. McEwan, and Read Pukkila-Worley
14. Measuring Phagocytosis in Bone Marrow-Derived Macrophages and Peritoneal Macrophages with Aging
Ryan Lu, Nirmal K. Sampathkumar, and Berenice A. Benayoun
15. Chromatin Immunoprecipitation from Caenorhabditis elegans Somatic Cells
Mintie Pu and Siu Sylvia Lee
16. Transcriptional Profiling of C. elegans Adult Cells and Tissues with Age
Rachel Kaletsky and Coleen T. Murphy
17. Measuring Tissue-specific Autophagy Flux in Adult Caenorhabditis elegans
Jessica T. Chang, Malene Hansen, and Caroline Kumsta
18. Assay Development and Measurement of the Aging Biomarker Humanin
Brendan Miller and Junxiang Wan
19. Staining and Quantification of -amyloid Pathology in Transgenic Mouse Models of Alzheimer's Disease
Amy Christensen and Christian J. Pike
20. Multimodal Imaging of Cerebral Microhemorrhages and White Matter Degradation in Geriatric Patients with Mild Traumatic Brain Injury
Maria Calvillo, Di Fan, and Andrei Irimia
21. Detection of HNE Modification of Proteins in Aging Mouse Tissues: A Western Blot-based Approach
Hongqiao Zhang, Natalie Lyn, Amin Haghani, and Henry Jay Forman
22. Measurements of Hydrogen Peroxide and Oxidative DNA Damage in a Cell Model of Premature Aging
Juan Manuel Iglesias-Pedraz and Lucio Comai
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