Vaccine adjuvants : methods and protocols

Author(s)

    • Davies, Gwyn

Bibliographic Information

Vaccine adjuvants : methods and protocols

edited by Gwyn Davies

(Springer protocols)(Methods in molecular biology / John M. Walker, series editor, 626)

Humana Press, c2010

  • : pbk

Available at  / 1 libraries

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Note

Includes bibliographical references and index

"Softcover re-print of the Hardcover 1st edition 2010"--T.p. verso

Contents of Works

  • Immunology of vaccine adjuvants / Carla M.S. Ribeiro and Virgil E.J.C. Schijns
  • Preclinical development of AS04 / Nathalie Garçon
  • Nonclinical safety assessment of vaccines and adjuvants / Jayanthi J. Wolf, Catherine V. Kaplanski,and Jose A.Lebron
  • Aluminum adjuvants : preparation, application, dosage, and formulation with Antigen / Erik B. Lindblad and Niels E. Schønberg
  • Freund's complete and incomplete adjuvants,preparation, and quality control standards for experimental laboratory animals use / Duncan E.S. Stewart-Tull
  • Liposomal adjuvants : preparation and formulation with antigens / Jean Haensler
  • Micro/nanoparticle adjuvants : preparation and formulation with antigens / Padma Malyala and Manmohan Singh
  • Adjuvant activity on human cells in vitro / Dominique De Wit and Michel Goldman
  • Adjuvant activity on murine and human macrophages / Valerie Quesniaux,Francois Erard,and Bernhard Ryffel
  • In vitro effects of adjuvants on B cells / Jörg Vollmer and Hanna Bellert
  • NKT cell responses to glycolipid activation / Josianne Nitcheu Tefit, Gwyn Davies and Vincent Serra
  • Tracking dendritic cells n vivo / Catherine M. Rush and James M. Brewer
  • Adjuvant effects on antibody titre / Barry Walker and Ian Feavers
  • Functional antibody assays / Ian Feavers and Barry Walker
  • Determining adjuvant activity on T-cell function in vivo : Th cells / Thomas Lindenstrom,Peter Andersen,and Else Marie Agger
  • Quantitative multiparameter assays to measure the effect of adjuvants on human antigen-specific CD8 T-cell responses / Laurent Derré ... [et al.]
  • Large-animal model for establishing E/T ratio of adjuvants / Luuk A. Th. Hilgers
  • Determining the activity of mucosal adjuvants / Barbara C. Baudner and Giuseppe Del Giudice
  • Adjuvant activity of cytokines / Michael G. Tovey and Christophe Lallemand

Description and Table of Contents

Description

Spanning from discoveries in fundamental immunology to industrial and commercial concerns, the study of vaccine adjuvants has developed into an exciting area of work with great, vital potential in innovating techniques in which adjuvants may steer the immune system towards the responses required by unmet vaccination needs. In Vaccine Adjuvants: Methods and Protocols, expert researchers in the field provide clear and concise guidance on how to go about assessing the activity of adjuvant products. Rather than describing individual adjuvants, the volume strives to include detailed, practical information on measuring the responses produced by adjuvants in order to be relevant to the widest array of experiments. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and versatile, Vaccine Adjuvants: Methods and Protocols will enable those already pursuing vaccine adjuvant research, while also serving to stimulate discussion on how to best standardize adjuvant testing in order to facilitate meaningful comparisons, and above all, to aid in the prediction of which new products will most effectively and safely help to solve the current challenges in vaccination.

Table of Contents

1. Immunology of Vaccine Adjuvants Carla M.S. Ribeiro and Virgil E.J.C. Schijns 2. Preclinical Development of AS04 Nathalie Garcon 3. Nonclinical Safety Assessment of Vaccines and Adjuvants Jayanthi J. Wolf, Catherine V. Kaplanski, and Jose A. Lebron 4. Aluminum Adjuvants: Preparation, Application, Dosage, and Formulation with Antigen Erik B. Lindblad and Niels E. Schonberg 5. Freund's Complete and Incomplete Adjuvants, Preparation, and Quality Control Standards for Experimental Laboratory Animals Use Duncan E.S. Stewart-Tull 6. Liposomal Adjuvants: Preparation and Formulation with Antigens Jean Haensler 7. Micro/Nanoparticle Adjuvants: Preparation and Formulation with Antigens Padma Malyala and Manmohan Singh 8. Adjuvant Activity on Human Cells in vitro Dominique De Wit and Michel Goldman 9. Adjuvant Activity on Murine and Human Macrophages Valerie Quesniaux, Francois Erard, and Bernhard Ryffel 10. In vitro Effects of Adjuvants on B Cells Joerg Vollmer and Hanna Bellert 11. NKT Cell Responses to Glycolipid Activation Josianne Nitcheu Tefit, Gwyn Davies, and Vincent Serra 12. Tracking Dendritic Cells in vivo Catherine M. Rush and James M. Brewer 13. Adjuvant Effects on Antibody Titre Barry Walker and Ian Feavers 14. Functional Antibody Assays Ian Feavers and Barry Walker 15. Determining Adjuvant Activity on T Cell Function in vivo: Th Cells Thomas Lindenstrom, Peter Andersen, and Else Marie Agger 16. Quantitative Multiparameter Assays to Measure the Effect of Adjuvants on Human Antigen Specific CD8 T Cell Responses Laurent Derre, Camilla Jandus, Petra Baumgaertner, Vilmos Posevitz,Estelle Devevre, Pedro Romero, and Daniel E. Speiser 17. Large-Animal Model for Establishing E/T-Ratio of Adjuvants Luuk A. Th. Hilgers 18. Determining the Activity of Mucosal Adjuvants Barbara C. Baudner and Giuseppe Del Giudice 19. Adjuvant Activity of Cytokines Michael G. Tovey and Christophe Lallemand

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