Arabidopsis protocols

著者

書誌事項

Arabidopsis protocols

edited by Jose J. Sanchez-Serrano and Julio Salinas

（Methods in molecular biology / John M. Walker, series editor, 2200）（Springer protocols）

Humana Press, c2021

4th ed

大学図書館所蔵 件 / 全3件

この図書・雑誌をさがす

注記

Includes bibliographical references and index

内容説明・目次

内容説明

This fourth edition compiles the most recent methodologies developed to exploit the Arabidopsis genome. Chapters detail access to public resources, to genetic, cell biology, biochemical and physiological techniques, and sections on genome, transcriptome, proteome, metabolome and other whole-system approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Arabidopsis Protocols, Fourth Edition aims to ensure successful results in the further study of this vital field.

目次

Part I: Arabidopsis Resources 1. Handling Arabidopsis and other Brassicaceae: Growth, Preservation of Seeds, Transformation and Genetic Crosses Christopher Calhoun, Deborah Crist, Emma Knee, Julie Miller, Eva Nagy, and David Somers 2. Bioinformatic Tools in Arabidopsis Research G. Alex Mason, Alex Canto-Pastor, Siobhan M. Brady, and Nicholas J. Provart Part II: Genetic Techniques in Arabidopsis 3. Analyses of Natural Variation: Field Experiments and Nucleotide Diversity for Your Favorite Gene Carlos Alonso-Blanco, Belen Mendez-Vigo, and F. Xavier Pico 4. Non-sterile Grafting Methods for Arabidopsis Nien-Chen Huang, and Tien-Shin Yu 5. CRISPR/Cas9-based Genome Editing Toolbox for Arabidopsis thaliana Daisuke Miki, Gaurav Zinta, Fangnan Peng, Zhengyan Feng, and Jian-Kang Zhu 6. Transient Transformation of A. thaliana Seedlings by Vacuum Infiltration Cesar Bernat-Silvestre, Vanessa Vieira, Fernando Aniento, and M Jesus Marcote 7. Transient Expression of Fluorescent Fusion Proteins in Arabidopsis Protoplasts Yonglun Zeng, Changyang Ji, Youshun Lin, and Liwen Jiang 8. Photosynthetic and Heterotrophic Cell Suspension Cultures Enrico Cortese, Luca Carraretto, Barbara Baldan, and Lorella Navazio 9. Genome-wide Association Studies in Arabidopsis thaliana: Statistical Analysis and Network-Based Augmentation of Signals Tak Lee and Insuk Lee Part III: Molecular and Cell biological Techniques in Arabidopsis 10. Visualising and Measuring Single Locus Dynamics in Arabidopsis thaliana Anis Meschichi and Stefanie Rosa 11. Identification and Quantification of Small RNAs Di Sun, Zeyang Ma, Jiaying Zhu, and Xiuren Zhang 12. Flow Cytometry and Sorting in Arabidopsis David W. Galbraith and Guiling Sun 13. Live Imaging of Arabidopsis Leaf and Vegetative Meristem Development Monica Pia Caggiano, Xiulian Yu, Carolyn Ohno, Pia Sappl, and Marcus G. Heisler 14. Using Genetically Encoded Fluorescent Biosensors for Quantitative in vivo Imaging Akira Yoshinari, Jacob Moe-Lange, Thomas J. Kleist, Heather N. Cartwright, David A. Quint, David W. Ehrhardt, Wolf B. Frommer, and Masayoshi Nakamura 15. Using Tripartite Split-sfGFP for the Study of Membrane Protein-Protein Interactions Tzu-Yin Liu 16. High-pressure Freezing and Freeze Substitution for Transmission Electron Microscopy Imaging and Immunogold-labeling Marisa S. Otegui 17. Atomic Force Microscopy to Study Cell Wall Mechanics in Plants Mateusz Majda 18. Identification and Characterization of Reproductive Mutations in Arabidopsis Maria Flores-Tornero, Stefanie Sprunck, and Thomas Dresselhaus Part IV: Biochemical and Physiological Techniques in Arabidopsis 19. Metabolomic Analysis of Natural Variation in Arabidopsis Si Wu, Saleh Alseekh, Yariv Brotman, and Alisdair R. Fernie 20. Untargeted Metabolomics of Arabidopsis Stomatal Immunity Lisa David, Jianing Kang, and Sixue Chen 21. Biotin-based Proximity Labeling Of Protein Complexes in Planta Madiha Khan, Rajagopal Subramaniam, and Darrell Desveaux 22. Phosphoproteomic Analysis of Plant Membranes Lin Xi, Waltraud X. Schulze, and Xu Na Wu 23. RNA-binding Protein Immunoprecipitation and High Throughput Sequencing Tino Koester and Dorothee Staiger

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