Arabidopsis protocols
著者
書誌事項
Arabidopsis protocols
(Methods in molecular biology / John M. Walker, series editor, 2200)(Springer protocols)
Humana Press, c2021
4th ed
大学図書館所蔵 全3件
  青森
  岩手
  宮城
  秋田
  山形
  福島
  茨城
  栃木
  群馬
  埼玉
  千葉
  東京
  神奈川
  新潟
  富山
  石川
  福井
  山梨
  長野
  岐阜
  静岡
  愛知
  三重
  滋賀
  京都
  大阪
  兵庫
  奈良
  和歌山
  鳥取
  島根
  岡山
  広島
  山口
  徳島
  香川
  愛媛
  高知
  福岡
  佐賀
  長崎
  熊本
  大分
  宮崎
  鹿児島
  沖縄
  韓国
  中国
  タイ
  イギリス
  ドイツ
  スイス
  フランス
  ベルギー
  オランダ
  スウェーデン
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  アメリカ
注記
Includes bibliographical references and index
内容説明・目次
内容説明
This fourth edition compiles the most recent methodologies developed to exploit the Arabidopsis genome. Chapters detail access to public resources, to genetic, cell biology, biochemical and physiological techniques, and sections on genome, transcriptome, proteome, metabolome and other whole-system approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Arabidopsis Protocols, Fourth Edition aims to ensure successful results in the further study of this vital field.
目次
Part I: Arabidopsis Resources
1. Handling Arabidopsis and other Brassicaceae: Growth, Preservation of Seeds, Transformation and Genetic Crosses
Christopher Calhoun, Deborah Crist, Emma Knee, Julie Miller, Eva Nagy, and David Somers
2. Bioinformatic Tools in Arabidopsis Research
G. Alex Mason, Alex Canto-Pastor, Siobhan M. Brady, and Nicholas J. Provart
Part II: Genetic Techniques in Arabidopsis
3. Analyses of Natural Variation: Field Experiments and Nucleotide Diversity for Your Favorite Gene
Carlos Alonso-Blanco, Belen Mendez-Vigo, and F. Xavier Pico
4. Non-sterile Grafting Methods for Arabidopsis
Nien-Chen Huang, and Tien-Shin Yu
5. CRISPR/Cas9-based Genome Editing Toolbox for Arabidopsis thaliana
Daisuke Miki, Gaurav Zinta, Fangnan Peng, Zhengyan Feng, and Jian-Kang Zhu
6. Transient Transformation of A. thaliana Seedlings by Vacuum Infiltration
Cesar Bernat-Silvestre, Vanessa Vieira, Fernando Aniento, and M Jesus Marcote
7. Transient Expression of Fluorescent Fusion Proteins in Arabidopsis Protoplasts
Yonglun Zeng, Changyang Ji, Youshun Lin, and Liwen Jiang
8. Photosynthetic and Heterotrophic Cell Suspension Cultures
Enrico Cortese, Luca Carraretto, Barbara Baldan, and Lorella Navazio
9. Genome-wide Association Studies in Arabidopsis thaliana: Statistical Analysis and Network-Based Augmentation of Signals
Tak Lee and Insuk Lee
Part III: Molecular and Cell biological Techniques in Arabidopsis
10. Visualising and Measuring Single Locus Dynamics in Arabidopsis thaliana
Anis Meschichi and Stefanie Rosa
11. Identification and Quantification of Small RNAs
Di Sun, Zeyang Ma, Jiaying Zhu, and Xiuren Zhang
12. Flow Cytometry and Sorting in Arabidopsis
David W. Galbraith and Guiling Sun
13. Live Imaging of Arabidopsis Leaf and Vegetative Meristem Development
Monica Pia Caggiano, Xiulian Yu, Carolyn Ohno, Pia Sappl, and Marcus G. Heisler
14. Using Genetically Encoded Fluorescent Biosensors for Quantitative in vivo Imaging
Akira Yoshinari, Jacob Moe-Lange, Thomas J. Kleist, Heather N. Cartwright, David A. Quint, David W. Ehrhardt, Wolf B. Frommer, and Masayoshi Nakamura
15. Using Tripartite Split-sfGFP for the Study of Membrane Protein-Protein Interactions
Tzu-Yin Liu
16. High-pressure Freezing and Freeze Substitution for Transmission Electron Microscopy Imaging and Immunogold-labeling
Marisa S. Otegui
17. Atomic Force Microscopy to Study Cell Wall Mechanics in Plants
Mateusz Majda
18. Identification and Characterization of Reproductive Mutations in Arabidopsis
Maria Flores-Tornero, Stefanie Sprunck, and Thomas Dresselhaus
Part IV: Biochemical and Physiological Techniques in Arabidopsis
19. Metabolomic Analysis of Natural Variation in Arabidopsis
Si Wu, Saleh Alseekh, Yariv Brotman, and Alisdair R. Fernie
20. Untargeted Metabolomics of Arabidopsis Stomatal Immunity
Lisa David, Jianing Kang, and Sixue Chen
21. Biotin-based Proximity Labeling Of Protein Complexes in Planta
Madiha Khan, Rajagopal Subramaniam, and Darrell Desveaux
22. Phosphoproteomic Analysis of Plant Membranes
Lin Xi, Waltraud X. Schulze, and Xu Na Wu
23. RNA-binding Protein Immunoprecipitation and High Throughput Sequencing
Tino Koester and Dorothee Staiger
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