Peptide nucleic acids : methods and protocols
著者
書誌事項
Peptide nucleic acids : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, v. 2105）（Springer protocols）
Humana Press, c2020
3rd ed
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
This third volume exemplifies and illustrates exciting advances in peptide nucleic acids (PNA) chemistry, and serves as a vital complement to the first and second edition of the book. Chapters focus on in vivo properties and behavior and applications of PNA while providing contributions on both chemistry and nucleic acid recognition. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Peptide Nucleic Acid: Methods and Protocols, Third Edition aims to ensure successful results in the further study of this vital field.
目次
1. Fmoc-based Assembly of PNA Oligomers: Manual and Microwave-assisted Automated Synthesis
Ashif Yasin Shaikh, Anna Mette Hansen, and Henrik Franzyk
2. A Robust Method for Preparing Optically Pure MiniPEG-Containing Gamma PNA Monomers
Wei-Che Hsieh and Danith H. Ly
3. Synthesis of Pyrrolidinyl PNA and Its Site-specific Labeling at Internal Positions by Click Chemistry
Boonsong Ditmangklo, Penthip Muangkaew, Kotchakorn Supabowornsathit, and Tirayut Vilaivan
4. Synthesis of PNA-peptide Conjugates as Functional SNARE Protein Mimetics
Barbara E. Hubrich, Patrick M. Menzel, Benedikt Kugler, and Ulf Diederichsen
5. Lipid-modified Peptide Nucleic Acids - Synthesis and Application to Programmable Liposome Fusion
Philipp M. G. Loeffler, Alexander Rabe, and Stefan Vogel
6. Facile Preparation of PNA-Peptide Conjugates with a Polar Maleimido-thioether Linkage
Anna Mette Hansen, Ashif Yasin Shaikh, and Henrik Franzyk
7. PNA-Encoded Synthesis (PES) and DNA Display of Small Molecule Libraries
Jacques Saarbach, Sofia Barluenga, and Nicolas Winssinger
8. Transcription Inhibition by PNA-induced R-loops
Boris P. Belotserkovskii, Sum-yan Ng, and Philip C. Hanawalt
9. Nucleobase-Modified Triplex-Forming Peptide Nucleic Acids for Sequence-Specific Recognition of Double-Stranded RNA
Nikita Brodyagin, Dziyana Hnedzko, James A MacKay, and Eriks Rozners
10. In Vitro Cellular Delivery of Peptide Nucleic Acid (PNA)
Takehiko Shiraishi, Mahdi Ghavami, and Peter E. Nielsen
11. Reactive Quantum Dot-based FRET Systems for Target-catalyzed Detection of RNA
Oleksandr Zavoiura, Ute Resch-Genger, and Oliver Seitz
12. Peptide Nucleic Acids for MicroRNAs Targeting
Roberto Gambari, Jessica Gasparello, Enrica Fabbri, Monica Borgatti, Anna Tamanini, and Alessia Finotti
13. Detection of Microorganisms by Fluorescence in situ Hybridization using Peptide Nucleic Acid
Ricardo Oliveira, Carina Almeida, and Nuno F. Azevedo
14. PNA Antisense Targeting in Bacteria. Determination of Antibacterial Activity (MIC) of PNA-peptide Conjugates
Lise Goltermann and Peter E. Nielsen
15. In vivo Administration of Splice Switching PNAs using the mdx Mouse as a Model System Camilla Brolin, Ernest Wee Kiat Lim, and Peter E. Nielsen
16. Near Infrared in vivo Whole Body Fluorescence Imaging of PNA Ernest Wee Kiat Lim, Camilla Brolin, and Peter E. Nielsen
17. Poly(lactic-co-glycolic acid) Nanoparticle Delivery of Peptide Nucleic Acids In Vivo
Stanley N. Oyaghire, Elias Quijano, Alexandra S. Piotrowski-Daspit, W. Mark Saltzman, and Peter M. Glazer
18. Preparation of Conjugates for Affibody-based PNA-mediated Pretargeting
Mohamed Altai, Anzhelika Vorobyeva, Vladimir Tolmachev, Amelie Eriksson Karlstroem, and Kristina Westerlund
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