Circadian clocks : methods and protocols

Author(s)

• Brown, Steven A.

Bibliographic Information

Circadian clocks : methods and protocols

edited by Steven A. Brown

（Methods in molecular biology / John M. Walker, series editor, 2130）（Springer protocols）

Humana Press, c2021

Note

Includes bibliographical references and index

Description and Table of Contents

Description

This volume presents techniques used by researchers from all branches of biology to study daily changes at a molecular level in many physiological systems. The chapters are organized into three parts and cover topics such as measuring and modeling physiological and behavioral rhythms; genome-wide analyses in circadian biology, and imaging and manipulating brain clocks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Circadian Clocks: Methods and Protocols is a valuable tool for any researcher interested in learning more about this developing field.

Table of Contents

Preface: Designing a Circadian Experiment Table of Contents... Contributing Authors... Part I Measuring and Modeling Physiological and Behavioral Rhythms 1. Real-Time In Vitro Fluorescence Anisotropy of the Cyanobacterial Circadian Clock Joel Heisler, Archana Chavan, Yong-Gang Chang, and Andy LiWang 2. Inductively Coupled Plasma Mass Spectrometry for Elemental Analysis in Circadian Biology Alessandra Stangherlin, Jason Day, and John O'Neill 3. The Assessment of Circadian Rhythms within the Immune System Chloe C. Nobis, Marc Cuesta, Jean-Francois Daudelin, Genevieve Dubeau-Laramee, Diane B. Boivin, Nathalie Labrecque, and Nicolas Cermakian 4. Measuring Circadian Rhythms in Human Cells Ngoc-Hien Du and Steven A. Brown 5. Measuring the Effects of Circadian Rhythm-Related Manipulations on Depression-Like Behavior in Rodents: Forced Swim and Tail Suspension Tests Chelsea A. Vadnie, Lauren M. DePoy, and Colleen A. McClung 6. Asking the Clock: How to Use Information from Questionnaires for Circadian Phenotyping Celine Vetter, Eva C. Winnebeck, and Till Roenneberg 7. Simple Kinetic Models in Molecular Chronobiology J. Patrick Pett, Pal O. Westermark, and Hanspeter Herzel Part II Genome-Wide Analyses in Circadian Biology 8. Searching Novel Clock Genes using RNAi-Based Screening Bert Maier, Stephan Lorenzen, Anna-Marie Finger, Hanspeter Herzel, and Achim Kramer 9. Chromatin Immunoprecipitation (ChIP) from Mouse Liver Nuclei Sara S. Fonseca Costa and Jurgen A. Ripperger 10. Using GRO-seq to Measure Circadian Transcription and Discover Circadian Enhancers Bin Fang, Dongyin Guan, and Mitchell A. Lazar 11. Circadian Metabolomics from Breath Steven A. Brown and Pablo M-L Sinues 12. A Lipidomics View of Circadian Biology Rona Aviram, Chunyan Wang, Xianlin Han, and Gad Asher 13. Circadian Lipidomics: Analysis of Lipid Metabolites Around-The-Clock Ursula Loizides-Mangold, Volodymyr Petrenko, and Charna Dibner 14. Phosphoproteome and Proteome Sample Preparation from Mouse Tissues for Circadian Analysis Franziska Bruning, Sean J. Humphrey and Maria S. Robles 15. Circadian Phosphorylation of CLOCK and BMAL1 Hikari Yoshitane and Yoshitaka Fukada Part III Imaging and Manipulating Brain Clocks 16. Fluorescence Live Imaging of Drosophila Circadian Pacemaker Neurons Virginie Sabado and Emi Nagoshi 17. Monitoring Electrical Activity in Drosophila Circadian Output Neurons Annika F. Barber and Amita Sehgal 18. Light Input to the Mammalian Circadian Clock Adam A. Dannerfjord, Laurence A. Brown, Russell G. Foster, and Stuart N. Peirson 19. Acute In Vivo Multi-Electrode Recordings from the Mouse Suprachiasmatic Nucleus Joshua Mouland, Lauren Walmsley, Timothy M. Brown, and Robert J. Lucas 20. Perforated Multi-Electrode Array Recording in Hypothalamic Brain Slices Mino D. C. Belle, Beatriz Bano-Otalora and Hugh D. Piggins 21. Collection of Mouse Brain Slices for Bioluminescence Imaging of Circadian Clock Networks Jennifer A. Evans, David K. Welsh, and Alec J. Davidson 22. Computational Analysis of PER2::LUC Imaging Data Tanya L. Leise 23. Electrophysiological Approaches to Studying the Suprachiasmatic Nucleus Stephan Michel, Takahiro J. Nakamura, Johanna H. Meijer, and Christopher S. Colwell 24. Optogenetic Methods for the Study of Circadian Rhythms Jeff R. Jones, Michael C. Tackenberg, and Douglas G. McMahon

by "Nielsen BookData"

Details

• NCID: BC04841605
• ISBN:
  • 9781071603802
• Country Code: us
• Title Language Code: eng
• Text Language Code: eng
• Place of Publication: New York
• Pages/Volumes: xxii, 342 p.
• Size: 26 cm
• Classification:
  • DC23 : 612.022
• Subject Headings:
  • LCSH : Circadian rhythms
• Parent Bibliography ID:
  • BA10794673
  • BA88873632