The hippo pathway : methods and protocols

著者

    • Hergovich, Alexander

書誌事項

The hippo pathway : methods and protocols

edited by Alexander Hergovich

（Methods in molecular biology / John M. Walker, series editor, 1893）（Springer protocols）

Humana Press, c2019

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

This detailed book covers numerous expert methodologies to examine Hippo signaling on the structural, molecular, cellular, and organismal level. Beginning with a section on Drosophila genetics, the volume continues with parts exploring molecular and cell biological studies of the Hippo pathway, the use of structural biology and biochemistry, and a brief look at the Hippo pathway in mouse models. Written for the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, The Hippo Pathway: Methods and Protocols equips newcomers and specialists alike with key methodologies to accurately define the status of Hippo signaling in their experimental settings.

目次

Part I: The Hippo Pathway: Drosophila Genetics Lead the Way 1. The Power of Drosophila Genetics: The Discovery of the Hippo Pathway Rewatee Gokhale and Cathie M. Pfleger 2. Drosophila Genetics: The Power of Genetic Mosaic Approaches Mardelle Atkins 3. Drosophila Genetics: Analysis of Tissue Growth in Adult Tissues Alexander D. Fulford and Paulo S. Ribeiro 4. Live Imaging of Hippo Pathway Components in Drosophila Imaginal Discs Jiajie Xu, Ting Su, Sherzod A. Tokamov, and Richard G. Fehon 5. Localization of Hippo Signaling Components in Drosophila by Fluorescence and Immunofluorescence Cordelia Rauskolb and Kenneth D. Irvine 6. Bimolecular Fluorescence Complementation (BiFC) in Tissue Culture and in Developing Tissues of Drosophila to Study Protein-Protein Interactions Yurika Matsui and Zhi-Chun Lai Part II: Molecular and Cell Biology Studies of the Hippo Pathway 7. Immunohistochemistry to Study YAP in Human Tissue Samples Franziska Haderk, Victor Olivas, and Trever G. Bivona 8. Immunofluorescence Study of Endogenous YAP in Mammalian Cells Valentina Rausch and Carsten G. Hansen 9. Immunofluorescence Microscopy to Study Endogenous TAZ in Mammalian Cells Nathan M. Kingston, Andrew M. Tilston-Lunel, Julia Hicks-Berthet, and Xaralabos Varelas 10. Nuclear/Cytoplasmic Fractionation to Study Hippo Effectors Maria Chatzifrangkeskou and Eric O'Neill 11. Luciferase Reporter Assays to Determine YAP/TAZ Activity in Mammalian Cells Sirio Dupont 12. Quantitative Real-Time PCR to Measure YAP/TAZ Activity in Human Cells Xiaolei Cao and Bin Zhao 13. HTRF (R) Total and Phospho-YAP(Ser127) Cellular Assays Diana Zindel, Claire Vol, Odile Lecha, Isabelle Bequignon, Merve Bilgic, Marion Vereecke, Fabienne Charrier-Savournin, Maite Romier, Eric Trinquet, Jean-Philippe Pin, Julie Pannequin, Thomas Roux, Elodie Dupuis, and Laurent Prezeau 14. Studying YAP-Mediated 3D Morphogenesis Using Fish Embryos and Human Spheroids Yoichi Asaoka, Hitoshi Morita, Hiroko Furumoto, Carl-Philipp Heisenberg, and Makoto Furutani-Seiki 15. Regulation of YAP/TAZ Activity by Mechanical Cues: An Experimental Overview Sirio Dupont 16. CRISPR-Mediated Approaches to Regulate YAP/TAZ Levels Ryan J. Quinton and Neil J. Ganem 17. Hippo Pathway Regulation by Tyrosine Kinases Nina Reuven, Matan Shanzer, and Yosef Shaul Part III: Structure Biology and Biochemistry to Study the Hippo Pathway 18. Structural and Biochemical Analyses of the Core Components of the Hippo Pathway Lisheng Ni and Xuelian Luo 19. Isothermal Titration Calorimetry Assays to Measure Binding Affinities In Vitro Kui Lin and Geng Wu 20. GST Pull-Down Assay to Measure Complex Formations Sun-Yong Kim and Toshio Hakoshima 21. Determining the Phosphorylation Status of Hippo Components YAP and TAZ Using Phos-tag Rui Chen, Steven W. Plouffe, and Kun-Liang Guan 22. Quantifying the Kinase Activities of MST1/2 Niamh A. O'Driscoll and David Matallanas 23. Measuring the Kinase Activities of the LATS/NDR Protein Kinases Alexander Hergovich 24. MST1/2 Kinase Assays Using Recombinant Proteins Marta Gomez, Yavuz Kulaberoglu, and Alexander Hergovich Part IV: The Hippo Pathway and Mouse Models 25. Visualizing HIPPO Signaling Components in Mouse Early Embryonic Development Tristan Frum and Amy Ralston 26. The Hippo Signaling Pathway in Regenerative Medicine Lixin Hong, Yuxi Li, Qingxu Liu, Qinghua Chen, Lanfen Chen, and Dawang Zhou

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