Protein self-assembly : methods and protocols
著者
書誌事項
Protein self-assembly : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 2039）（Springer protocols）
Humana Press, c2020
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注記
"corrected publication 2020"--T.p. verso
Includes bibliographical references and index
内容説明・目次
内容説明
This volume explores experimental and computational approaches to measuring the most widely studied protein assemblies, including condensed liquid phases, aggregates, and crystals. The chapters in this book are organized into three parts: Part One looks at the techniques used to measure protein-protein interactions and equilibrium protein phases in dilute and concentrated protein solutions; Part Two describes methods to measure kinetics of aggregation and to characterize the assembled state; and Part Three details several different computational approaches that are currently used to help researchers understand protein self-assembly. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Thorough and cutting-edge, Protein Self-Assembly: Methods and Protocols is a valuable resource for researchers who are interested in learning more about this developing field.
目次
Preface...Table of Contents...Contributing Authors...
Part I Measuring Protein-Protein Interactions and Protein Phase Diagrams
1. Measuring Non-Specific Protein-Protein Interactions by Dynamic Light ScatteringDaniel Corbett, Jordan W. Bye, and Robin Curtis
2. Light Scattering to Quantify Protein-Protein Interactions at High Protein ConcentrationsMahlet A. Woldeyes, Cesar Calero-Rubio, Eric M. Furst, and Christopher J. Roberts
3. Quantitative Evaluation of Protein Solubility in Aqueous Solutions by PEG-Induced Liquid-Liquid Phase SeparationYing Wang and Ramil F. Latypov
4. Measuring Protein SolubilityNeer Asherie
Part II Measuring Protein Self-Association, Aggregation, and Crystallization
5. Integral caa3-Cytochrome c Oxidase from Thermus thermophiles: Purification and CrystallizationOrla Slattery, Sabri Cherrak, and Tewfik Soulimane
6. Aggregation Profiling of C9orf72 Dipeptide Repeat Proteins Transgenically Expressed in Drosophila melanogaster using an Analytical Ultracentrifuge Equipped with Fluorescence DetectionBashkim Kokona, Nicole R. Cunningham, Jeanne M. Quinn, and Robert Fairman
7. Sample Preparation and Size Analysis of C9orf72 Dipeptide Repeat Proteins Expressed in Drosophila melanogaster using Semi-Denaturing Agarose Gel ElectrophoresisNicole R. Cunningham, Bashkim Kokona, Jeanne M. Quinn, and Robert Fairman
8. The Use of High Performance Liquid Chromatography for the Characterization of the Unfolding and Aggregation of Dairy ProteinsSophie Gaspard and Andre Brodkorb
9. Differential Scanning Calorimetry to Quantify Heat Induced Aggregation in Concentrated Protein SolutionMatthew R. Jacobs, Mark Grace, Alice Blumlein, and Jennifer J. McManus
10. Nanoparticle Tracking Analysis to Examine the Temperature-Induced Aggregation of ProteinsSvenja Sladek, Kate McComiskey, Anne Marie Healy, and Lidia Tajbar
11. Evaluation of Temporal Aggregation Processes using Spatial Intensity Distribution AnalysisZahra Rattray, Egor Zindy, Kara M. Buzza, and Alain Pluen
12. Fluorescence Correlation Spectroscopy for Particle Sizing in Highly Concentrated Protein SolutionsJudith J. Mittag, Matthew R. Jacobs, and Jennifer J. McManus
13. Size Determination of Protein Oligomers/Aggregates Using Diffusion NMR SpectroscopyPancham S. Kandiyal, Ji Yoon Kim, Daniel L. Fortunati, and K.H. Mok
Part III Computational Approached to Measure Protein Self-Assembly
14. Patchy Particle models to Understand Protein Phase BehaviorNicoletta Gnan, Francesco Sciortino, and Emanuela Zaccarelli
15. Obtaining Soft Matter Models of Proteins and their Phase BehaviorIrem Altan and Patrick Charbonneau
16. Binding Free Energies of Conformationally Disordered Peptides through Extensive Sampling and End-Point MethodsMatthew G. Nixon and Elisa Fadda
17. Atomistic Simulation Tools to Study Protein Self-AggregationDeniz Meneksedag-Erol and Sarah Rauscher
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