Expressed protein ligation : methods and protocols
著者
書誌事項
Expressed protein ligation : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 2133）（Springer protocols）
Humana Press, c2020
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
This book provides a comprehensive overview of Expressed Protein Ligation (EPL), detailing methods and protocols to generate site-specifically modified proteins. Chapters include an overview of the protein semi-synthesis field, as well as related areas that have contributed to the development of EPL such as protein splicing and peptide synthesis. Following the introductory chapters, the rest of the book guides readers through protocols to perform EPL reactions, methods to synthesize peptide thioesters and to perform peptide and protein ligations, label proteins inside living cells, protocols for the semi-synthesis of phorphorylated, glycosylated and ubiquitylated proteins, synthesis and assembly of assymetrically modified nucleosomes, use of ligation auxiliaries and synthesis of cyclic proteins, as well as novel desulfurization strategies and use of selective Cys side chain protection to obtain precisely modified proteins.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Expressed Protein Ligation: Methods and Protocols will ensure successful implementation of protein semi-synthesis methods to further study the structure and function of proteins.
目次
1. Methods and Applications of Expressed Protein Ligation
Zhipeng A. Wang and Philip A. Cole
2. Protein Splicing: From the Foundations to the Development of Biotechnological Applications
Alejandro Romero-Casanas, Veronica Gordo, Jessica Castro, and Marc Ribo
3. Identification, Characterization, and Optimization of Split Inteins Neel H. Shah and Adam J. Stevens
4. Methods to Study the Structure and Catalytic Activity of cis-splicing Inteins
Jing Zhao, Zhenming Du, Chunyu Wang, and Kenneth V. Mills
5. Expressed Protein Ligation: General Experimental Protocols Joshua Diaz and Jean-Philippe Pellois
6. Peptide Hydrazides as Thioester Equivalents for the Chemical Synthesis of Proteins
Yu Wang and Yi-Ming Li
7. Native Chemical Ligation Via N-Acylurea Thioester Surrogates Obtained by Fmoc Solid Phase Peptide Synthesis
Judith Pala-Pujadas and Juan B. Blanco-Canosa
8. Fused Split Inteins: Tools for Introducing Multiple Protein Modifications
Byung Joon Lim, Raymond F. Berkeley, and Galia T. Debelouchina
9. Streamlined Expressed Protein Ligation: Site-specific Antibody-drug Conjugate
Silvia Frutos
10. In vivo Histone Labeling Using Ultrafast trans-Splicing Inteins
Nicholas A. Prescott and Yael David
11. Site-Directed Modification of Yeast-Produced Proteins Using Expressed Protein Ligation
Benjamin J. Umlauf and Eric V. Shusta
12. Site-specific Phosphorylation of PDZ Domains
Sana Ma, Kristian Stromgaard and Louise S. Clemmensen
13. Semisynthesis and Reconstitution of Nucleosomes Carrying Asymmetric Histone Modifications
Nora Guidotti and Beat Fierz
14. Facile Semisynthesis of Ubiquitylated Peptides with the Ligation Auxiliary 2-Aminooxyethanethiol
Caroline E. Weller and Champak Chatterjee
15. Investigating the Effects of O-GlcNAc Modifications in Parkinson's Disease using Semi-synthetic -synuclein
Ana Galesic and Matthew R. Pratt
16. Recombinant Expression of Cyclotides Using Expressed Protein Ligation
Maria Jose Campbell, Jingtan Su, and Julio A. Camarero
17. Highly Precise Protein Semisynthesis through Ligation-Desulfurization Chemistry in Combination with Phenacyl Protection of Native Cysteines
Somnath Mukherjee, Maria Matveenko, and Christian F.W. Becker
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