Crop breeding : genetic improvement methods
Author(s)
Bibliographic Information
Crop breeding : genetic improvement methods
（Methods in molecular biology / John M. Walker, series editor, 2264）（Springer protocols）
Humana Press, c2021
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Note
Includes bibliographical references and index
Description and Table of Contents
Description
This volume describes breeding methods for the development of biparental and multiparental mapping populations. Chapters detail lab protocols for high-throughput isolation of nucleic acids and metabolites, high performing genotyping approaches, mapping strategies for QTLs, mutation identifications, computational, bioinformatic pipelines, tissue culture-based and transformation methods for androgenesis, ploidy modification, and RNA interference. Additional chapters highlight recent developed genome editing protocols including CRISPR and TALEN methods and methodologies for in-field/in-soil plant phenotyping. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Crop Breeding: Genetic Improvement Methods aims to ensure successful results in the further study of this vital field.
Table of Contents
1. Methods of Development of Biparental Mapping Populations in Horticultural Crops
Pasquale Tripodi
2. Multiparental Population in Crops: Methods of Development and Dissection of Genetic Traits
Isidore Diouf and Laura Pascual
3. Using Metabolomics to Assist Plant Breeding
Saleh Alseekh and Alisdair R. Fernie
4. High Throughput DNA Isolation in Vegetable Crops for Genomics Applications
Pasquale Tripodi and Giovanna Festa
5. High Resolution Melting Analysis as a Tool for Plant Species Authentication Liliana Grazina, Joana Costa, Joana S. Amaral, and Isabel Mafra
6. Specific-locus Amplified Fragment Sequencing (SLAF-seq) as High-Throughput SNP Genotyping Methods
Zhangsheng Zhu, Binmei Sun, and Jianjun Lei
7. Effective Mapping by Sequencing to Isolate Causal Mutations in the Tomato Genome
Fernando J. Yuste-Lisbona, Jose M. Jime nez-Go mez, Carmen Capel, and Rafael Lozano
8. Association Mapping in Plants
Pawan L. Kulwal and Ravinder Singh
9. Practical Workflow from High Throughput Genotyping to Genomic Estimated Breeding Values (GEBVs)
Contaldi Felice, Cappetta Elisa, and Esposito Salvatore
10. Guidelines for Setting up a mRNA Sequencing Experiment and Best Practices for Bioinformatic Data Analysis Teresa Rosa Galise, Salvatore Esposito, and Nunzio D'Agostino
11. RNA Interference (RNAi) in Tomato Crop Reseserch
Pasquale Termolino
12. Protoplast Based Method for Genome Editing in Tetraploid Potato
Alessandro Nicolia, Ann-Sofie Falt, Per Hofvander, and Mariette Andersson
13. The Double Layer Method to the Genesis of Androgenic Plants in Anemone coronaria
Andrea Copetta and Marina Laura
14. Ploidy Modification for Plant Breeding Using in vitro Organogenesis: a Case in Eggplant
Edgar Garcia-Fortea, Ana Garcia-Perez, Esther Gimeno-Paez, Marina Martinez-Lopez, Santiago Vilanova, Pietro Gramazio, Jaime Prohens and Mariola Plazas
15. Assembly of TALEN and mTALE-Act for Plant Genome Engineering
Aimee A. Malzahn and Yiping Qi
16. Genome Editing to Achieve the Crop Ideotype in Tomato
Tomas Cermak, Karla Gasparini, Zoltan Kevei, and Agustin Zsoegoen
17. Root System Phenotying of Soil-Grown Plants via RGB and Hyperspectral Imaging
Gernot Bodner, Mouhannad Alsalem, and Alireza Nakhforoosh
18. Light Drones for Basic In-field Phenotyping and Precision Farming Applications: RGB Tools Based on Image Analysis
Federico Pallottino, Simone Figorilli, Cristina Cecchini, and Corrado Costa
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