Histidine phosphorylation : methods and protocols
Author(s)
Bibliographic Information
Histidine phosphorylation : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, v. 2077）（Springer protocols）
Humana Press, c2020
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Includes index
Description and Table of Contents
Description
This volume details the current understanding of roles and regulation on histidine phosphorylation, describing methods for the characterization of protein phosphorylation on histidine. Chapters guide readers through in vitro systems, cell-based systems, comprehensive background review articles on histidine kinases and phosphatases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Histidine Phosphorylation: Methods and Protocols aims to ensure successful results in the further study of this rapidly growing field.
Table of Contents
1. Protein Dynamics in Phosphoryl-transfer Signaling Mediated by Two-component Systems
Felipe Trajtenberg and Alejandro Buschiazzo
2. A High-throughput Strategy for Recombinant Protein Expression and Solubility Screening in Escherichia coli - A Case of Sensor Histidine Kinase
Agnieszka Szmitkowska, Blanka Pekarova, and Jan Hejatko
3. SDS-PAGE and Dot Blot Autoradiography: Tools for Quantifying Histidine Kinase Autophosphorylation
Jonathan T. Fischer, Ilana Heckler, and Elizabeth M. Boon
4. A Quantitative Method for the Measurement of Protein Histidine Phosphorylation
Paul V. Attwood
5. Analysis of 1- and 3-phosphohistidine (pHis) Protein Modification using Model Enzymes Expressed in Bacteria
Alice K. M. Clubbs Coldron, Dominic P. Byrne, and Patrick A. Eyers
6. Determination of Phosphohistidine Stoichiometry in Histidine Kinases by Intact Mass Spectrometry
Lauren J. Tomlinson, Alice K. M. Clubbs Coldron, Patrick A. Eyers, and Claire E. Eyers
7. Protein Phosphohistidine Phosphatases of the HP Superfamily
Daniel J. Rigden
8. In Vitro Assays for Measuring Protein Histidine Phosphatase Activity
Brandon S. McCullough and Amy M. Barrios
9. Structural and Functional Characterization of Autophosphorylation in Bacterial Histidine Kinases
Laura Miguel-Romero, Cristina Mideros-Mora, Alberto Marina, and Patricia Casino
10. Manipulation of Bacterial Signalling using Engineered Histidine Kinases
Kimberly A. Kowallis, Samuel W. Duvall, Wei Zhao, and W. Seth Childers
11. Development of a Light-Dependent Protein Histidine Kinase
Aleksandra Bury and Klaas J. Hellingwerf
12. Empirical Evidence of Cellular Histidine Phosphorylation by Immunoblotting using pHis mAbs
Rajasree Kalagiri, Kevin Adam, and Tony Hunter
13. Immunohistochemistry (IHC): Chromogenic Detection of 3-Phosphohistidine Proteins in Formaldehyde-Fixed, Frozen Mouse Liver Tissue Sections
Natalie Luhtala and Tony Hunter
14. Subcellular Localisation of Histidine Phosphorylated Proteins through Indirect Immunofluorescence
Kevin Adam and Tony Hunter
15. High throughput Characterisation of Histidine Phosphorylation sites using UPAX and Tandem Mass Spectrometry
Gemma Hardman and Claire E. Eyers
16. Proteome Bioinformatics Methods for Studying Histidine Phosphorylation
Andrew R Jones and Oscar Martin Camacho
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