Clinical and preclinical models for maximizing healthspan : methods and protocols
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Bibliographic Information
Clinical and preclinical models for maximizing healthspan : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 2138）（Springer protocols）
Humana Press, c2020
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Note
Includes bibliographical references and index
Description and Table of Contents
Description
This book presents a series of protocols in multiple disease areas affected by the aging process along with several methods which have shown progress in nutrient- or intervention-based approaches to maximize the healthspan. Of interest to researchers in the areas of chronic disease, gerontology, physical exercise, and nutrition as well as to clinical scientists, physicians, and pharmacologists, this volume also provides important information on disease mechanisms and novel drug targets as each protocol will be presented in the context of specific chronic diseases or different therapeutic areas. Written for the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Clinical and Preclinical Models for Maximizing Healthspan: Methods and Protocols is an ideal guide for preclinical and clinical researchers working toward the discoveries needed to lead to a world society in which individuals are not only living longer lives but more productive and healthier ones.
Table of Contents
Part I: Reviews
1. The Impact of New Biomarkers and Drug Targets on Age-Related Disorders
Paul C. Guest
2. Vitamin D and Muscle Sarcopenia in Aging
Behnaz Abiri and Mohammadreza Vafa
3. Quantitative Assays of Plasma Apolipoproteins
Anne Poljak, Mark W. Duncan, Tharusha Jayasena, and Perminder S. Sachdev
4. Protocol for the Use of the Ketogenic Diet in Preclinical and Clinical Practice
Ann-Katrin Kraeuter, Paul C. Guest, and Zoltan Sarnyai
Part II: Protocols
5. An In Vivo/Ex Vivo Study Design to Investigate Effects of Chronic Conditions and Therapeutic Compounds on Adipose Stem Cells in Animal Models
Hanel Sadie-Van Gijsen, Liske Kotze-Hoerstmann, and Barbara Huisamen
6. Model for Studying the Effects of Chronic Metabolic Disease on Endogenous Bone Marrow Stem Cell Populations
Yashar Mehrbani Azar, Maria Jacoba Kruger, Dalene de Swardt, Michelle Maartens, Ascentia Mathapelo Seboko, William Frank Ferris, and Mari van de Vyver
7. Investigating Alcohol Behavior and Physiology Using Drosophila Melanogaster
Aliza K. De Nobrega, Kristine V. Luz, Katherine N. Lyons, and Lisa C. Lyons
8. Using Genome-Editing Tools to Develop a Novel In Situ Coincidence Reporter Assay for Screening ATAD3A Transcript Inhibitors
Liwei Lang and Yong Teng
9. Visualizing and Evaluating Cancer Cell Growth and Invasion by a Novel 3D Culture System
Chloe Shay, Liwei Lang, and Yong Teng
10. Exploiting Plug-and-Play Electrochemical Biosensors to Determine the Role of FGF19 in Sorafenib-Mediated Superoxide and Nitric Oxide Production in Hepatocellular Carcinoma Cells
Lixia Gao and Yong Teng
11. Proteomic Analysis of the Anoikis-Resistant Human Breast Cancer Cell Lines
Chareeporn Akekawatchai, Sittiruk Roytrakul, Narumon Phaonakrop, Janthima Jaresitthikunchai, and Sarawut Jitrapakdee
12. Real-Time PCR Analysis of Metabolism-Related Genes in a Long-Lived Model of C. Elegans
Sumino Yanase
13. Clinical Assessment of the NADome as Biomarkers for Healthy Aging
Tharusha Jayasena, Sonia Bustamante, James Clement, Robert Welschinger, Gideon A. Chaplan, Perminder S. Sachdev, and Nady Braidy
14. 2D Gel Electrophoresis Combined with Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Analysis of Eye Lens to Identify Biomarkers of Age-Related Cataract
Paul C. Guest
15. Testing the Effects of Dietary Seafood Consumption on Depressive Symptoms
Maximus Berger, G. Paul Amminger, Robyn McDermott, Paul C. Guest, and Zoltan Sarnyai
16. Assays for Monitoring the Effects of Nicotinamide Supplementation on Mitochondrial Activity in Saccharomyces Cerevisiae
Ivan Orlandi and Marina Vai
17. Measurement of a Surrogate Biomarker for Arginine Vasopressin Secretion is Association with Physiometric and Molecular Biomarkers of Aging
Paul C. Guest, Hans-Gert Bernstein, Henrik Dobrowolny, Katrin Borucki, Sabine Westphal, and Johann Steiner
18. Liquid Chromatography Tandem Mass Spectrometry Analysis of Proteins Associated with Age-Related Disorders in Human Pituitary Tissue
Paul C. Guest and Daniel Martins de-Souza
19. Coenzyme Q10 Assessment and the Establishment of a Neuronal Cell Model of CoQ10 Deficiency
Robert Heaton, Kate Duberley, and Iain P. Hargreaves
20. Analyzing Mitochondrial Function in Brown Adipocytes with a Bioenergetic Analyzer
Justin Darcy, Chih-Hao Wang, and Yu-Hua Tseng
21. Quantitative Analysis of DNA Methylation by Bisulfite Sequencing
Vasily V. Ashapkin, Lyudmila I. Kutueva, and Boris F. Vanyushin
22. Histomorphometric Analysis of Anti-Aging Properties on Rat Skin
Idha Kusumawati, Kresma Oky Kurniawan, Subhan Rullyansyah, and Eka Pramyrtha Hestianah
23. Integration of qRT-PCR and Immunohistochemical Techniques for mRNA Expression and Localization of m1AChR in the Brain of Aging Rat
S. Asha Devi and S. Abhijit
24. Randomized Study Design to Test Effects of Vitamin D and Omega-3 Fatty Acid Supplementation as Adjuvant Therapy in Colorectal Cancer Patients
Fatemeh Haidari, Behnaz Abiri, Masood Iravani, Kambiz Ahmadi-Angali, and Mohammadreza Vafa
25. Effect of Vitamin D Supplementation on Muscle Strength, Muscle Function, and Body Composition in Vitamin D-Deficient Middle-Aged Women
Behnaz Abiri, Mohsen Dehghani, and Mohammadreza Vafa
26. The Association of Food Intake and Physical Activity with Body Composition, Muscle Strength, and Muscle Function in Postmenopausal Women
Mohammadreza Vafa, Behnaz Abiri, and Mohsen Dehghani
27. Absolute Quantification of Plasma Apolipoproteins for Cardiovascular Disease Risk Prediction
Betul Ozdemir, Zeliha Selamoglu, and Nady Braidy
28. Multiplex Analysis of Circulating Hormone Levels in Rat Models of Age-Related Diseases
Paul C. Guest
29. Proteomic Analysis of Brain Tissue from a Chronic Model of Stress Using a Combined 2D Gel Electrophoresis and Mass Spectrometry Approach
Paul C. Guest
30. Proteomic Analysis of Rat Hippocampus for Studies of Cognition and Memory Loss with Aging
Paul C. Guest, Hassan Rahmoune, and Daniel Martins-de Souza
31. Brain Proteomic Analysis on the Effects of the Antidepressant Fluoxetine
Paul C. Guest
32. MK-801 Treatment of Oligodendrocytes as a Cellular Model of Aging
Paul C. Guest
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