Vibrio cholerae : methods and protocols

著者

• Sikora, Aleksandra E.

書誌事項

Vibrio cholerae : methods and protocols

edited by Aleksandra E. Sikora

（Methods in molecular biology / John M. Walker, series editor, 1839）（Springer protocols）

Humana Press, c2018

内容説明・目次

内容説明

This volume looks at the importance of V. cholerae as an infection agent, and explores the latest techniques used to study it. The chapters in this book cover topics such as the biology of V. cholerae; laboratory maintenance and culturing; various animals models developed to explain pathogenesis and factors for environmental survival; and holistic approaches that include proteomics, metabolomics, and high-throughput screening. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Vibrio cholerae: Methods and Protocols is a valuable resource for both novice microbiologists who want to focus their studies on V. cholerae, and experienced researchers and teachers who want to improve their knowledge and skills in this growing field.

目次

Preface... Table of Contents... Contributing Authors... 1. Laboratory Culturing Techniques and Maintenance of Vibrio cholera Kyle D. Brumfield, Bailey M. Carignan, and Mike S. Son 2. Genotypic and Phenotypic Assays to Distinguish Vibrio cholera Biotype Kyle D. Brumfield, Bailey M. Carignan, and Mike S. Son 3. Preparation of Vibrio cholera Samples for RNA-seq Analysis Jyl S. Matson 4. Random Transposon Mutagenesis of Vibrio cholera Jyl S. Matson 5. Metabolomics of Vibrio cholera Yusuke Minato, Jay S. Kirkwood, and Claudia C. Hase 6. Natural Cotransformation and Multiplxed Genome Editing by Natural Transformation (MuGENT) of Vibrio cholera Ankur B. Dalia 7. Vibrio cholera Chromatin Immunoprecipitation Julio C. Ayala, Jorge A. Benitez, and Anisia J. Silva 8. Fly Models of Vibrio cholera Infection and Colonization Alexandra E. Purdy 9. Danio rerio as a Native Host Model for Understanding Pathophysiology of Vibrio cholera Kristie C. Mitchell and Jeffrey H. Withey 10. Transposon Sequencing of Vibrio cholera in the Infant Rabbit Model of Cholera Lauren M Shull and Andrew Camilli 11. Isolation of Outer Membrane Vesicles Including their Quantitative and Qualitative Analyses Paul Kohl, Franz G. Zingl, Tom Eichmann, and Stefan Schild 12. Utilization of Vibrio cholera as a Model Organism to Screen natural Product Libraries for Identification of New Antibodies Aleksandra E. Sikora, Richard Tehan, and Kerry PcPhail 13. Infant Mouse Model of Vibrio cholera Infection and Colonization Jyl S. Matson 14. Methods for Assessments of Collagenolytic Activity of the Vibrio cholera Extracellular Proteases, Purification of Secreted Collagenase VchC, and Extraction of Type I Collagen from Fish Skin Bo R. Weber and Aleksandra E. Sikora 15. Proteomics of Vibrio cholerarea Ryszard A. Zielke

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詳細情報

• NII書誌ID(NCID): BC05925722
• ISBN:
  • 9781493986842
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: New York
• ページ数/冊数: x, 180 p
• 大きさ: 26 cm
• 親書誌ID:
  • BA10794673
  • BA88873632