Cancer genomics and proteomics : methods and protocols

書誌事項

Cancer genomics and proteomics : methods and protocols

edited by Narendra Wajapeyee

(Methods in molecular biology / John M. Walker, series editor, 1176)

Humana Press, c2014

2nd ed

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

Cancer Genomics and Proteomics: Methods and Protocols, Second Edition includes methods for the analyses of cancer genome and proteome that have illuminated us about the changes in cancer cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cancer Genomics and Proteomics: Methods and Protocols, Second Edition seeks to aid scientists in the further study into various aspects of tumor initiation and progression.

目次

1. Genome-Wide Mapping Of RNA Pol-II Promoter Usage In Mouse Tissues By Chip-Seq Sharmistha Pal, Ravi Gupta And Ramana V. Davuluri 2. Using Native Chromatin Immunoprecipitation To Interrogate Histone Variant Protein Deposition In Embryonic Stem Cells Zito Tseng, Tao Wu, Yifei Liu, Mei Zhong, And Andrew Xiao 3. Reduced Representation Bisulfite Sequencing To Identify Global Alteration In The DNA Methylation Arvindhan Nagarajan, Christine Roden And Narendra Wajapeyee 4. A High-Throughput Microrna Expression Profiling System Yanwen Guo, Stephen Mastriano And Jun Lu 5. Using Pooled Mir30-Shrna Library For Cancer Lethal And Synthetic Lethal Screens Liam Changwoo Lee, Shaojian Gao, Qiuning Li, And Ji Luo 6. Genome-Wide RNA Interference Screening To Identify Regulators Of Epigenetic Silencing Of A Tumor Suppressor Gene Matteo Forloni, Thuy Ho And Narendra Wajapeyee 7. A Diphtheria Toxin Negative Selection In RNA Interference Screening Zhi Sheng, Susan F Murphy, Sujuan Guo And Michael R Green 8. Cancer Metabolism: Crosstalk Between Signaling And O-Glcnacylation Christina M. Ferrer And Mauricio J. Reginato 9. Targeted Genome Modification Via Triple Helix Formation Adele S. Ricciardi, Nicole A. Mcneer, Kavitha K. Anandalingam, W. Mark Saltzman, Peter M. Glazer 10. Synthesis Of Stabilized Alpha-Helical Peptides Federico Bernal And Samuel G. Katz 11. Arginine-Grafted Biodegradable Polymer: A Versatile Transfection Reagent For Both DNA And Sirna Jagadish Beloor, Hye Yeong Nam, Sang-Kyung Lee And Priti Kumar 12. Using Laco Arrays To Monitor DNA Double-Strand Break Dynamics In Live Schizosaccharomyces Pombe Cells Bryan A. Leland And Megan C. King 13. Zebrafish As A Platform To Study Tumor Progression Corrie A. Painter And Craig J. Ceol 14. Clonal Screens To Find Modifiers Of Partially Penetrant Phenotypes In C. Elegans Michael E. Hurwitz 15. Serum Profiling Using Protein Microarrays To Identify Disease Related Antigens. Donald Sharon And Michael Snyder 16. Interrogation Of In Vivo Protein-Protein Interactions Using Transgenic Mouse Models And Stable Isotope Labeling Anwesha Dey, Jiansheng Wu, Donald S. Kirkpatrick 17. New Biophysical Methods To Study The Membrane Activity Of Bcl-2 Proteins Stephanie Bleicken And Ana J. Garcia-Saez 18. Purification Of Recombinant 2XMBP Tagged Human Proteins From Human Cells Ryan Jensen 19. Computational Analysis In Cancer Exome Sequencing Perry Evans, Yong Kong, And Michael Krauthammer 20. Matrix Factorization Methods For Integrative Cancer Genomics Shihua Zhang And Xianghong Jasmine Zhou 21. Computational Methods For DNA Copy-Number Analysis Of Tumors Jude Kendall And Alexander Krasnitz

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