The TNF superfamily : methods and protocols
著者
書誌事項
The TNF superfamily : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 2248）（Springer protocols）
Humana Press, c2021
2nd ed
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
This second edition focuses on various techniques to investigate aspects of the TNF Superfamily members in health and disease. Chapters detail protocols on the signaling process of TNF family members, technical examples to investigating the role of TNF family members in physiopathologies, modulation of TNF signaling by pathogens, experimental applications of TNF-reporter mice, methodologies for various assays of TNF family members and the production of recombinant molecules. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and accessible, The TNF Superfamily: Methods and Applications, Second Edition serves to aid researchers investigating this key family of proteins.
目次
1. Signaling in TNFSF15-mediated Suppression of VEGF Production in Endothelial Cells
Huanyu Zhao and Qiangzhe Zhang
2. Sensitization of Airway Epithelial Cells to Toxin-Induced Death by TNF Superfamily Cytokines
Claire Reynolds-Peterson, Dylan J Ehrbar, Susanne M McHale, Timothy J. LaRocca, and Nicholas J Mantis
3. Inhibition of Chondroitin Sulfate Proteoglycans by APRIL
Mashal Claude Ahmed and Bertrand Huard
4. Induction of Antigen-independent Proliferation of Regulatory T-cells by TNF superfamily ligands OX40L and GITRL
Prabhakaran Kumar, Zarema H Arbieva, Mark Maienschein-Cline, Balaji B Ganesh, Suresh Ramasamy, and Bellur S Prabhakar
5. Transition from TNF-induced Inflammation to Death Signaling
Swati Choksi, Gourav Choudhary, and Zheng-gang Liu
6. Analysis of FcgR-Dependent Agonism Of Antibodies Specific for Receptors of the Tumor Necrosis Factor (TNF) Receptor Superfamily (TNFRSF)
Juliane Medler and Harald Wajant
7. Generation and Evaluation of Bispecific anti-TNF Antibodies Based on Single-Chain VHH Domains
M.A. Nosenko, K-S.N. Atretkhany, V.V. Mokhonov, S.A. Chuvpilo, D.V. Yanvarev, M.S. Drutskaya, S.V. Tillib, and S.A. Nedospasov
8. In Vitro Physical and Functional Interaction Assays to Examine the Binding of Progranulin Derivative Atsttrin to TNFR2 and its anti-TNF activity
Wenyu Fu, Aubryanna Hettinghouse, and Chuan-ju Liu
9. Fluorescence Based TNFR1 Biosensor for Monitoring Receptor Structural and Conformational Dynamics and Discovery of Small Molecule Modulators
Chih Hung Lo, Tory M. Schaaf, David D. Thomas, and Jonathan N. Sachs
10. Production of Multi-Subtype Influenza Virus-Like Particles by Molecular Fusion with BAFF or APRIL for Vaccine Development
Ting-Hsuan Chen, Jo-Yu Hong, Chia-Chyi Liu, Chung-Chu Chen, Jia-Tsrong Jan, and Suh-Chin Wu
11. Experimental Methods for the Immunological Characterization of Paradoxical Psoriasis Reactions Induced by TNF- Biologics
Martina Morelli, Claudia Scarponi, Stefania Madonna, and Cristina Albanesi
12. Methods for the Administration of EDAR Pathway Modulators in Mice
Sonia Schuepbach-Mallepell, Christine Kowalczyk-Quintas, Angela Dick, Mahya Eslami, Michele Vigolo, Denis J. Headon, Michael Cheeseman, Holm Schneider, and Pascal Schneider
13. Analysis of Ligand-Receptor Interactions Using Bioluminescent TNF Superfamily (TNFSF) Ligand Fusion Proteins
Kirstin Kucka, Juliane Medler, and Harald Wajant
14. Monitoring Atsttrin-mediated Inhibition of TNF /NF- Activation through in vivo Bioluminescence Imaging
Aubryanna Hettinghouse, Wenyu Fu, and Chuan-ju Liu
15. The use of Murine Infection Models to Investigate the Protective Role of TNF in Central Nervous System Tuberculosis
Nai-Jen Hsu and Muazzam Jacobs
16. Identification of CD137- and CD137L-expressing Cells in EL-4 Tumor
Sang W. Kang, Hong R. Cho, and Byungsuk Kwon
17. Expression Profiling of Tumor Necrosis Factor Superfamily Ligands mRNA in
Healthy and Injured Murine Kidneys
Bhawna Tomar and Shrikant R. Mulay
18Analysis of Lymphotoxin Alpha Expression in Human Retina and Generation of Expression Vectors to Functional Characterization of Polymorphisms in the Tumor Necrosis Factor Locus
Ricardo Usategui-Martin, Irene Rodriguez-Hernandez, Rogelio Gonzalez-Sarmiento, Eva M. Sobas, Jose Carlos Pastor Jimeno, and Salvador Pastor Idoate
19. Detection of TNF- Protein in Extracellular Vesicles Derived from Tumor Cells by Western Blotting
Tandressa Souza Berguetti, Raquel Ciuvalschi Maia, and Paloma Silva de Souza
20. Expression of TNFRs by B and T lymphocytes in Tumor-Draining Lymph Nodes
Atri Ghods, Abbas Ghaderi, and Fereshteh Mehdipour
21. Methods for Evaluation of TNF- Inhibition Effect
Kirti Hira and Sajeli A Begum
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