Cell cycle oscillators : methods and protocols
著者
書誌事項
Cell cycle oscillators : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 2329）（Springer protocols）
Humana Press, c2021
2nd ed
- : hbk
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注記
Includes bibliographical references
内容説明・目次
内容説明
This book brings together a unique collection of protocols that cover novel and specialized techniques as well as updated and improved adaptations of more standard procedures involving the cell cycle and its regulation by oscillatory networks, exploring recent progress in the field from both holistic and reductionist perspectives. The edition provides a space for researchers to highlight and explore the latest developments in molecular biology and biochemical techniques for studying oscillatory networks and to share these across the research community to facilitate further progress. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and up-to-date, Cell Cycle Oscillators: Methods and Protocols, Second Edition serves as an invaluable guide for those new to the field as well as the more experienced scientist.
Chapters 19 and 21 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
目次
1. Cell Cycle Control: A System of Interlinking Oscillators
Randy Y.C. Poon
2. Substrate Phosphorylation Rates as an In Vivo Measurement of Kinase Activity
Matthew P. Swaffer
3. Real Time Monitoring of APC/C-Mediated Substrate Degradation Using Xenopus laevis Egg Extracts
Julia Kamenz, Renping Qiao, Qiong Yang, and James E. Ferrell, Jr.
4. Fluorescent Peptide Biosensors for Probing CDK Kinase Activity in Cell Extracts
Morgan Pellerano and May C. Morris
5. Phosphatase and Kinase Substrate Specificity Profiling with Pooled Synthetic Peptides and Mass Spectrometry
Andrew G. DeMarco, Pete E. Pascuzzi, W. Andy Tao, and Mark C. Hall
6. Whole-Mount Immunostaining for the Identification of Histone Modifications in the S-Phase Nuclei of Arabidopsis Roots
Hirotomo Takatsuka and Masaaki Umeda
7. Construction of a Full-Length 3'UTR Reporter System for Identification of Cell-Cycle Regulating MicroRNAs
Dominika Kazmierczak and Per Hydbring
8. Purification of Cyclin-Dependent Kinase Fusion Complexes for In Vitro Analysis
Mardo Koivomagi
9. Optimizing Cell Synchronization Using Nocodazole or Double Thymidine Block
Arif A. Surani, Sergio L. Colombo, George Barlow, Gemma A. Foulds, and Cristina Montiel-Duarte
10. Highly Synchronous Mitotic Progression in Schizosaccharomyces pombe Upon Relief of Transient Cdc2-asM17 Inhibition
Pawan Singh, Lenka Halova, and Iain Michael Hagan
11. Elucidating Human Mitosis Using an Anaphase-Like Cell-Free System
Danit Wasserman, Sapir Nachum, Meirav Noach-Hirsh, Naomi Auerbach, Evelin Sheinberger-Chorni, Taylor P Enrico, Roxane Lahmi, Michael J. Emanuele, and Amit Tzur
12. EDU (5-Ethynyl-2'-Deoxyuridine)-Coupled-Fluorescence-Intensity Analysis: Determining Absolute Parameters of the Cell Cycle
Joao A. Ferreira, Marco Neves, Miguel Alpalhao, Pedro Pereira, Daniela Cunha, Fernando Ferreira, Rene Santus, Ana E. Sousa, and Paulo L. Filipe
13. High-Resolution Analysis of Centrosome Behavior During Mitosis
Vanessa Nunes, Margarida Dantas, Joana T. Lima, and Jorge G. Ferreira
14. Assaying Cell Cycle Progression via Flow Cytometry in CRISPR/Cas9-Treated Cells
Jonathan M. Geisinger and Tim Stearns
15. Use of Mitotic Protein Kinase Inhibitors and Phospho-Specific Antibodies to Monitor Protein Phosphorylation During the Cell Cycle
Isha Nasa, Greg B. Moorhead, and Arminja N. Kettenbach
16. Visualization of Radiation-Induced Cell Cycle Kinetics with a Fluorescent Ubiquitination-Based Cell Cycle Indicator (Fucci)
Atsushi Kaida and Masahiko Miura
17. Dynamic Behavior of Inactive X Chromosome Territory During the Cell Cycle as Revealed by H3K27me3-Specific Intracellular Antibody
Yuko Sato and Hiroshi Kimura
18. Analyzing Centrioles and Cilia by Expansion Microscopy
Dong Kong and Jadranka Loncarek
19. Analysis of Cell Cycle Progression in the Budding Yeast S. cerevisiae
Deniz Pirincci Ercan and Frank Uhlmann
20. Application of PALM Super Resolution Microscopy to the Analysis of Microtubule-Organizing Centers (MTOCs) in Aspergillus nidulans
Xiaolei Gao, Reinhard Fischer, and Norio Takeshita
21. Live Imaging and Analysis of Cilia and Cell Cycle Dynamics with the Arl13bCerulean-Fucci2a Biosensor and Fucci Tools
Melinda Van Kerckvoorde, Matthew J. Ford, Patricia L. Yeyati, Pleasantine Mill, and Richard L. Mort
22. Calorimetric Heat Dissipation Measurements of Developing Zebrafish Embryos
Jonathan Rodenfels and Karla M. Neugebauer
23. The Conditional Knockout Analogous System: CRISPR-Mediated Knock Out Together with Inducible Degron and Transcription-Controlled Expression
Hoi Tang Ma
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