RNA chaperones : methods and protocols
著者
書誌事項
RNA chaperones : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 2106）（Springer protocols）
Humana Press, 2020
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
This book provides a wide spectrum of methods to study RNA chaperones in vitro, at the single molecule level, and protocols useful for cell-based assays. Beginning with a section on a number of bacterial proteins for study, the volume also explores proteins from eukaryotic cells and how to delve into the complex interactions between RNA chaperones and the folding and unfolding of proteins. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, RNA Chaperones: Methods and Protocols serves as an ideal guide for scientists and students interested in RNA biology and RNA chaperones.
Chapter 3 is available Open Access under a Creative Commons Attribution 4.0 International License via link.springer.com.
目次
1. Biochemical Methods for the Study of the FinO Family of Bacterial RNA Chaperones
Hyeong Jin Kim, Steven Chaulk, David Arthur, Ross A. Edwards, and J.N. Mark Glover
2. Quantitative Analysis of RNA Chaperone Activity by Native Gel Electrophoresis and Fluorescence Spectroscopy
Subrata Panja, Ewelina M. Malecka, Andrew Santiago-Frangos, and Sarah A. Woodson
3. Fluorescent Molecular Beacons Mimicking RNA Secondary Structures to Study RNA Chaperone Activity
Pilar Menendez-Gil, Carlos J. Caballero, Cristina Solano, and Alejandro Toledo-Arana
4. Specific Nucleic Acid Chaperone Activity of HIV-1 Nucleocapsid Protein Deduced from Hairpin Unfolding
Micah J. McCauley, Ioulia Rouzina, and Mark C. Williams
5. Real-Time Fluorescence-Based Approaches to Disentangle Mechanisms of a Protein's RNA Chaperone Activity
Tobias Schmidt, Susann Friedrich, Ralph P. Golbik, and Sven-Erik Behrens
6. Use of tRNA-Mediated Suppression to Assess RNA Chaperone Function
Jennifer Porat and Mark A. Bayfield
7. Salt-Dependent Modulation of the RNA Chaperone Activity of RNA-Binding Protein La
Gunhild Sommer, Christina Sendlmeier, and Tilman Heise
8. Kinetic and Thermodynamic Analyses of RNA-Protein Interactions
Ryo Amano and Taiichi Sakamoto
9. Detection of MicroRNAs Released from Argonautes
Kyung-Won Min, J. Grayson Evans, Erick C. Won, and Je-Hyun Yoon
10. Mapping the RNA Chaperone Activity of the T. brucei Editosome Using SHAPE-Chemical Probing
W.-Matthias Leeder and H. Ulrich Goeringer
11. RNA Remodeling by RNA Chaperones Monitored by RNA Structure Probing
Susann Friedrich, Tobias Schmidt, and Sven-Erik Behrens
12. In Vivo RNA Chemical Footprinting Analysis in Archaea
Robert Knuppel, Martin Fenk, Michael Juttner, and Sebastien Ferreira-Cerca
13. RNA Structure Analysis by Chemical Probing with DMS and CMCT
Jose M. Andrade, Ricardo F. dos Santos, and Cecilia M. Arraiano
14. Disordered RNA Binding Region Prediction with DisoRDPbind
Christopher J. Oldfield, Zhenling Peng, and Lukasz Kurgan
15. Delivering Molecular Beacons via an Electroporation-Based Approach Enables Live-Cell Imaging of Single RNA Transcripts and Genomic Loci
Shiqi Mao, Yachen Ying, Xiaotian Wu, and Antony K. Chen
16. Site-Specific Dual-Color Labeling of Long RNAs
Meng Zhao, Richard Boerner, Roland K.O. Sigel, and Eva Freisinger
17. Single-Molecule FRET Assay for Studying Cotranscriptional RNA Folding
Heesoo Uhm and Sungchul Hohng
18. Characterizing Complex Nucleic Acid Interactions of LINE1 ORF1p by Single Molecule Force Spectroscopy
M. Nabuan Naufer and Mark C. Williams
19. Isolation and Analysis of Bacterial Ribosomes through Sucrose Gradient Ultracentrifugation
Ricardo F. dos Santos, Cecilia M. Arraiano, and Jose M. Andrade
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