T-helper cells : methods and protocols
著者
書誌事項
T-helper cells : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 2285）（Springer protocols）
Humana Press, c2021
大学図書館所蔵 件 / 全2件
-
該当する所蔵館はありません
- すべての絞り込み条件を解除する
注記
"Springer Science+Business Media, LLC, part of Springer Nature 2021"--T.p. verso
Includes bibliographical references and index
内容説明・目次
内容説明
The aim of this volume is to provide a comprehensive description of methods and protocols useful for the further study of T-helper cells. Chapters guide readers through T-helper cell recovery, molecular study, signal transduction pathways, T-cell manipulation and, last but not least, "omic" approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, T- Helper Cells: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field.
目次
1. Human and Murine T-Helper Cell Recovery from Organs and Tissues
Francesco Siracusa, Annette Lahmann, Anna Vanni, Filippo Cortesi, and Alessio Mazzoni
2. Identification and Purification of Human Memory T-Helper Cells from Peripheral Blood
Alessia Capone and Elisabetta Volpe
3. Transcription Factor Evaluation by Flow Cytometry
Daniela Fenoglio and Tiziana Altosole
4. Mass Cytometry Analysis of T-Helper Cells
Priyanka B. Subrahmanyam and Holden T. Maecker
5. Murine T-Helper Cell Differentiation and Plasticity
Francesco Siracusa, Franziska Muscate, and Laura Garcia Perez
6. Identification of Follicular T-Cell Subsets in Murine and Human Tissues
Annette Lahmann, Laura Bauer, and Andreas Hutloff
7. Analysis of T-Cells In Inflamed Non-Lymphoid Tissues
Laura Bauer and Andreas Hutloff
8. TCR V Evaluation by Flow Cytometry
Benedetta Peruzzi, Sara Bencini, and Roberto Caporale
9. Evaluation of Cytokine Production at the Single Cell Level by Flow Cytometry Upon Polyclonal Stimulation
Jakob Zimmermann
10. Evaluation of Secreted Cytokines by Multiplex Bead Based Assay (X MAP Technology, Luminex)
Letizia Lombardelli, Federica Logiodice, Ornela Kullolli, and Marie-Pierre Piccinni
11. Quantifying Antigen-specific T-Cells by Assessing their Antigen-Induced Proliferation
Aayushi Shah and Hyun-Dong Chang
12. Flow Cytometric Characterization of Human Antigen-Reactive T-Helper Cells
Carina Saggau, Alexander Scheffold, and Petra Bacher
13. Identification of Human Antigen-Specific CD4+ cells with Peptide-MHC Multimer Technologies
I-Ting Chow and William W. Kwok
14. Human T-cell Cloning by Limiting Dilution
Laura Maggi, Manuela Capone, and Alessio Mazzoni
15. Human Germinal Center Reaction: Assessing T-Follicular Helper-B cell Interactions In Vitro
Emeline Thevenon, Vanessa Cornacchione, Elisabetta Traggiai, and Francesca Rucci
16. Study of Signal Transduction Pathways by Phospho-Protein Evaluation Manuela Capone, Alessio Mazzoni, and Laura Maggi
17. The Study of Protein-DNA Interactions in CD4+ T Cells using ChIPmentation
Arnulf Hertweck and Richard G. Jenner
18. mRNA Transfection of T lymphocytes by Electroporation
Lea-Isabell Schwarze and Boris Fehse
19. Transduction of Human T-Cell Subsets with Lentivirus
Vivian C. W. Fung, Isaac Rosado-Sanchez, Megan K Levings
20. Plasmid- or Ribonucleoprotein-mediated CRISPR/Cas Gene Editing in Primary Murine T-Cells
Marianne Doelz, Romina Marone, and Lukas T. Jeker
21. Generation of Sequencing Libraries for Building Immune Cell Methylomes
Stefan Floess and Jochen Huehn
22. Gene Expression Analysis of T-Cells by Single Cell RNA-seq
Domenico Lo Tartaro, Sara De Biasi, Mattia Forcato, Lara Gibellini, and Andrea Cossarizza
23. Quantifying Proteome and Protein Modifications in Activated T-Cells by Multiplexed Isobaric Labeling Mass Spectrometry
Haiyan Tan, Daniel Bastardo Blanco, Boer Xie, Yuxin Li, Zhiping Wu, Hongbo Chi, and Junmin Peng
24. Metabolomics, Lipidomics and Immunometabolism
Fortunata Carbone, Sara Bruzzaniti, Clorinda Fusco, Alessandra Colamatteo, Teresa Micillo, Paola de Candia, Fabrizia Bonacina, Giuseppe Danilo Norata and Giuseppe Matarese
25. Experimental Mouse Models of Asthma and Analysis of CD4+ T Cells
William J. Branchett, Simone A. Walker, and Clare M. Lloyd
26. Murine T-Cell Transfer Colitis as a Model for Inflammatory Bowel Disease
Patrick Maschmeyer, Jakob Zimmermann, and Anja Andrea Kuhl
27. Experimental Autoimmune Encephalomyelitis
Clara Ballerini
「Nielsen BookData」 より