CiNii 図書 - Live cell imaging : methods and protocols

This detailed book highlights recent advances in molecular imaging techniques and protocols, designed to be immediately applicable in global bio-laboratories. The chapters are categorized into seven major groups according to the reporter materials, such as imaging with passive optical readouts, activatable bioluminescent probes, functional substrates and luciferases, organic fluorescent probes, BRET probes, FRET probes, as well as with advanced instrumentation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Live Cell Imaging: Methods and Protocols aims to direct and inspire researchers into creating smarter, next-generation imaging techniques that are truly quantitative, highly sensitive, and readily comprehended, in the effort to engender deeper understanding of biological systems and break new ground in the research fields of life science.

Part I: Imaging with Passive Optical Readouts 1. Fluorescent Labeling of the Nuclear Envelope without Relying on Inner Nuclear Membrane Proteins Toshiyuki Taniyama and Shinji Sueda 2. Quantitative Analysis of Membrane Receptor Trafficking Manipulated by Optogenetic Tools Osamu Takenouchi, Hideaki Yoshimura, and Takeaki Ozawa 3. Simultaneous Detection of Four Cell Cycle Phases with Live Fluorescence Imaging Bryce T. Bajar and Michael Z. Lin 4. A Murine Bone Metastasis Model Using Caudal Artery Injection and Bioluminescence Imaging Takahiro Kuchimaru and Shinae Kizaka-Kondoh 5. A New Lineage of Artificial Luciferases for Mammalian Cell Imaging Sung-Bae Kim and Rika Fujii 6. Use of Bacterial Luciferase as a Reporter Gene in Eukaryotic Systems Jittima Phonbuppha, Ruchanok Tinikul, and Pimchai Chaiyen Part II: Imaging with Activatable Bioluminescent Probes 7. Quantitative Determination and Imaging of G q Signaling in Live Cells via Split-Luciferase Complementation Timo Littmann, Takeaki Ozawa, and Gunther Bernhardt 8. A Split-Luciferase-Based Cell Fusion Assay for Evaluating the Myogenesis-Promoting Effects of Bioactive Molecules Qiaojing Li, Hideaki Yoshimura, and Takeaki Ozawa 9. Development of a Single Fluorescent Protein-Based Green Glucose Indicator by Semi-Rational Molecular Design and Molecular Evolution Marie Mita, Devina Wongso, Hiroshi Ueda, Takashi Tsuboi, and Tetsuya Kitaguchi Part III: Imaging with Functional Substrates and Luciferases 10. Near Infrared Bioluminescence Imaging of Animal Cells with Through-Bond Energy Transfer Cassette Masahiro Abe, Ryo Nishihara, Sung Bae Kim, and Koji Suzuki 11. Azide- and Dye-Conjugated Coelenterazine Analogues for Imaging Mammalian Cells Ryo Nishihara, Emi Hoshino, Yoshiki Kakudate, Koji Suzuki, and Sung-Bae Kim 12. Luciferase-Specific Coelenterazine Analogues for Optical Crosstalk-Free Bioassays Ryo Nishihara, Masahiro Abe, Koji Suzuki, and Sung-Bae Kim Part IV: Imaging with Organic Fluorescent Probes 13. Live Imaging of Virus-Infected Cells by Using a Sialidase Fluorogenic Probe Tadanobu Takahashi, Yuuki Kurebayashi, Tadamune Otsubo, Kiyoshi Ikeda, Akira Minami, and Takashi Suzuki 14. Live-Cell Imaging of Sirtuin Activity Using a One-Step Fluorescence Probe Mitsuyasu Kawaguchi and Hidehiko Nakagawa 15. Live-Cell Fluorescence Imaging of Microtubules by Using a Tau-Derived Peptide Hiroshi Inaba and Kazunori Matsuura 16. Discovery of the Environment-Sensitive Near-Infrared (NIR) Fluorogenic Ligand for 1-Adrenergic Receptors Imaging In Vivo Zhao Ma, Lupei Du, and Minyong Li 17. Rapid and Sensitive Detection of Cancer Cells with Activatable Fluorescent Probes for Enzyme Activity Kyohhei Fujita, Mako Kamiya, and Yasuteru Urano 18. Detection of Intracellular Reactive Oxidative Species Using the Fluorescent Probe Hydroxyphenyl Fluorescein Wataru Sugimoto, Daisuke Miyoshi, and Keiko Kawauchi 19. Development of Near-Infrared Fluorescent Mg2+ Probe and Application to Multi-Color Imaging of Intracellular Signals Yutaka Shindo, Yuma Ikeda, Yuki Hiruta, Daniel Citterio, and Kotaro Oka 20. Long-Term Mg2+ Imaging in Live Cells with a Targetable Fluorescent Probe Priya Ranjan Sahoo, Toshiyuki Kowada, and Shin Mizukami Part V: Imaging with BRET Probes 21. Highly Bright NIR-BRET System for Imaging Molecular Events in Live Cells Ryo Nishihara, Koji Suzuki, Sung-Bae Kim, and Ramasamy Paulmurugan 22. Ligand-Activatable BRET9 Probes for Imaging Molecular Events in Living Mammalian Cells Sung-Bae Kim, Rika Fujii, and Ramasamy Paulmurugan 23. Manipulation of Actin Cytoskeleton by Intracellular-Targeted ROS Generation Tetsuya Ishimoto and Hisashi Mori 24. Bioluminescence Imaging of Neuronal Network Dynamics Using Aequorin-Based Calcium Sensors Sandrine Picaud, Bertrand Lambolez, and Ludovic Tricoire 25. Method for Detecting Emission Spectral Change of Bioluminescent Ratiometric Indicators via a Smartphone Mitsuru Hattori, Tomoki Matsuda, and Takeharu Nagai 26. Bioluminescence Resonance Energy Transfer (BRET) Imaging in Living Cells: Image Acquisition and Quantification Hiroyuki Kobayashi and Michel Bouvier Part VI: Imaging with FRET Probes 27. GPCR Signaling Regulation in Dictyostelium Chemotaxis Yoichiro Kamimura and Masahiro Ueda 28. Live Cell Imaging Technique to Visualize DAMPs Release during Regulated Cell Death Mai Yamagishi and Yoshitaka Shirasaki 29. Time-Lapse Imaging of Necroptosis and DAMP Release at Single Cell Resolution Shin Murai, Yoshitaka Shirasaki, and Hiroyasu Nakano Part VII: Imaging with Advanced Instrumentation 30. High-Throughput Whole Plate Imaging of Cells for Multiple Biological Applications Uday Kumar Sukumar, Frezghi Habte, Tarik F. Massoud, and Ramasamy Paulmurugan 31. Pinhole Closure Improves Spatial Resolution in Confocal Scanning Microscopy Akira Kitamura 32. Total Workflows of the Single-Molecule Imaging Analysis in Living Cells: Tutorial Guidance to the Measurement of the Drug Effects on a GPCR Masataka Yanagawa and Yasushi Sako

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Live cell imaging : methods and protocols

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