Molecular toxicology protocols
著者
書誌事項
Molecular toxicology protocols
（Methods in molecular biology / John M. Walker, series editor, 2102）（Springer protocols）
Humana Press, c2020
3rd ed
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
This fully revised book includes new and improved protocols to analyze toxicity at the DNA, RNA, and protein levels using genomic, epigenomic, transcriptomic, and proteomic approaches. Sections of the book address key subjects such as bioinformatics and biostatistics, toxicant-induced lung inflammation and tumors, analysis of protein and gene expression and silencing, gene promoter methylation, detection of chromosomal and genome-wide damage, analysis of DNA-adducts and surrogate gene mutations, detection and characterization of cancer gene mutation, and many other areas of study. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and up-to-date, Molecular Toxicology Protocols, Third Edition will prove to play an important role in generating a better understanding of the field of toxicology and its sub-disciplines.
目次
- Part I: Bioinformatics and Biostatistics 1. Bioenergetic Analyses of In Vitro and In Vivo Samples to Guide Toxicological Endpoints Jonathan W. Boyd, Julia A. Penatzer, Nicole Prince, Julie V. Miller, Alice A. Han, and Holly N. Currie 2. Next Generation Sequencing (NGS) Application in Multi-Parameter Gene Expression Analysis Degeng Wang and Andrey L. Karamychev 3. Integrated Chip-Seq and RNA-Seq Data Analysis Coupled with Bioinformatics Approaches to Investigate Regulatory Landscape of Transcription Modulators in Breast Cancer Cells Jairo Ramos, Quentin Felty, and Deodutta Roy 4. Analysis of RNA Sequencing Data using CLC Genomics Workbench Chia-Hsin Liu and Y. Peter Di Part II: Toxicant-Induced Lung Inflammation and Lung Tumors 5. Using Bronchoalveolar Lavage to Evaluate Changes in Pulmonary Diseases Marissa E. Di, Dandan Yang, and Y. Peter Di 6. Fast and Efficient Measurement of Clinical and Biological Samples Using Immunoassay-Based Multiplexing Systems Yingze Zhang, Xiaoyun Li, and Y. Peter Di 7. Induction of Lung Tumors and Mutational Analysis in FVB/N Mice Treated with the Tobacco Carcinogen 4-(Methylnitrosamino)-1-(3-pyridyl)-1-Butanone Natalie J. Rothenberger and Laura P. Stabile Part III: Analysis of Protein and Gene Expression and Silencing 8. Two-Dimensional Difference Gel Electrophoresis: A Gel-Based Proteomic Approach for Protein Analysis Weimin Gao 9. Determination and Quantification of Bacterial Virulent Gene Expression Using Quantitative Real Time PCR Qiao Lin and Y. Peter Di 10. Using a Lentivirus-Based Inducible RNAi Vector to Silence a Gene Si Chen, Dongying Li, Zhen Ren, Dianke Yu, Baitang Ning, Nan Mei, and Lei Guo Part IV: Gene Promoter Methylation 11. Analysis of Toxicants-Induced Alterations in DNA Methylation by Methylation-Sensitive Random Amplified Polymorphic DNA-Polymerase Chain Reaction (MS-RAPD-PCR) Kamaleshwar P. Singh 12. Detection of DNA Methylation by MeDIP and MBDCap Assays: An Overview of Techniques Hang-Kai Hsu, Yu-I Weng, Pei-Yin Hsu, Tim H.-M. Huang, and Yi-Wen Huang Part V: Detection of Chromosomal and Genome-Wide Damage 13. Analysis of Telomere Length and Aberrations by Quantitative FISH Elise Fouquerel and Patricia Opresko 14. Detection of Loss of Heterozygosity in Tk-Deficient Mutants from L5178Y Tk+/--3.7.2C Mouse Lymphoma Cells Xiaoqing Guo, Ying Chen, Martha M. Moore, and Nan Mei 15. Detection of DNA Double-Strand Breaks and Chromosome Translocations Using Ligation-Mediated PCR and Inverse PCR Sheetal Singh, Shyh-Jen Shih, and Andrew T. M. Vaughan Part VI: Analysis of DNA-Adducts and Surrogate Gene Mutations 16. 32P-Postlabeling Analysis of DNA Adducts David H. Phillips and Volker M. Arlt 17. Determination of Mutational Spectra Induced by Environmental Toxicants in Complex Human Cell Populations Robert Gealy and Phouthone Keohavong 18. Detection of Pig-a Mutant Erythrocytes in the Peripheral Blood of Rats and Mice Vasily N. Dobrovolsky, Xuefei Cao, Javed A. Bhalli, and Robert H. Heflich 19. Analysis of In Vivo Mutation in the Hprt and Tk Genes of Mouse Lymphocytes Vasily N. Dobrovolsky, Joseph G. Shaddock, and Robert H. Heflich 20. Molecular Analysis of Mutations in the Human HPRT Gene Phouthone Keohavong, Liqiang Xi, and Stephen G. Grant 21. Simultaneous Quantification of t(14
- 18) and HPRT Exon 2/3 Deletions In Human Lymphocytes James C. Fuscoe Part VII: Detection and Characterization of Cancer Gene Mutation 22. Analysis of Mutations in K-ras and p53 Genes in Sputum and Plasma Samples Lifang Zhang, Wei-Min Gao, and Phouthone Keohavong 23. ACB-PCR Quantification of Low-Frequency Hotspot Cancer-Driver Mutations Meagan B. Myers, Karen L. McKim, Yiying Wang, Malathi Banda, and Barbara L. Parsons 24. Detection and Characterization of Oncogene Mutations in Preneoplastic and Early Neoplastic Lesions Toshinari Minamoto Part VIII: Analysis of DNA Damage and Repair Mechanisms 25. The Sister Chromatid Exchange (SCE) Assay Dawn M. Stults, Michael W. Killen, Paola Marco-Casanova, and Andrew J. Pierce 26. The Gene Cluster Instability (GCI) Assay for Recombination Michael W. Killen, Dawn M. Stults, Paola Marco-Casanova, and Andrew J. Pierce 27. New Perspectives on Unscheduled DNA Synthesis: Functional Assay for Global Genomic DNA Nucleotide Excision Repair Manasi R. Pimpley, Megan L. Foley, and Jean J. Latimer 28. Host Cell Reactivation: Assay for Actively Transcribed DNA Nucleotide Excision Repair Using Luciferase Family Expression Vectors Jowaher S. Alanazi and Jean J. Latimer Part IX: Analysis of Endoplasmic Reticulum Stress and miRNA/RNA Interaction 29. Methods for Establishing and Using a Stable Cell Line Expressing Both Gaussia Luciferase and Firefly Luciferase to Screen for Endoplasmic Reticulum Stress Zhen Ren, Si Chen, and Lei Guo 30. FREMSA: A Method That Provides Direct Evidence of the Interaction Between MicroRNA and mRNA Dianke Yu, Si Chen, Dongying Li, Bridgett Knox, Lei Guo, and Baitang Ning
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