Cancer cell signaling : methods and protocols
Author(s)
Bibliographic Information
Cancer cell signaling : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 2174）（Springer protocols）
Humana Press, c2021
3rd ed
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Note
Includes bibliographical references and index
Description and Table of Contents
Description
This fully updated edition provides the most recent advances in cancer cell signaling knowledge combined with some discussion of the current challenges and prospects in cancer therapy. Beginning with an important section on cancer resistance, the main obstacle to effective cancer therapy, the book continues with chapters exploring state-of-the-art methods in epigenetic control of cancer, metastasis promotion, as well as a variety of new technologies in the study of cancer. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and timely, Cancer Cell Signaling: Methods and Protocols, Third Edition serves as an ideal guide to researchers seeking to overcome the challenges in the vital field of cancer cell signaling.
Table of Contents
Part I: Cancer Resistance
1. Fighting Cancer Resistance: An Overview
Martha Robles-Flores
2. Prostate Cancer Spheroids: A Three-Dimensional Model for Studying Tumor Heterogeneity
Mauricio Rodriguez-Dorantes, Carlos David Cruz-Hernandez, Sergio Alberto Cortes-Ramirez, Jenie Marian Cruz-Burgos, Juan Pablo Reyes-Grajeda, Oscar Peralta-Zaragoza, and Alberto Losada-Garcia
3. Enrichment and Transcriptional Characterization of Stem Cells Isolated from Human Glioblastoma Cell Lines
Ana G. Pina-Medina, Ana M. Hernandez-Vega, Nestor F. Diaz, Ismael Mancilla-Herrera, and Ignacio Camacho-Arroyo
4. Reverse Docking for the Identification of Molecular Targets of Anticancer Compounds
Angel Jonathan Ruiz-Moreno, Alexander Doemling, and Marco Antonio Velasco-Velazquez
5. Mouse Model for Efficient Simultaneous Targeting of Glycolysis, Glutaminolysis, and De Novo Synthesis of Fatty Acids in Colon Cancer
Alejandro Schcolnik-Cabrera and Alfonso Duenas-Gonzalez
Part II: Epigenetic Control of Cancer
6. Developing a Portable Device for the Identification of miRNAs in Fluids
Alexander Asanov, Alicia Sampieri, and Luis Vaca
7. Methods for the Study of Long Non-Coding RNA in Cancer Cell Signaling
Yi Feng, Junjie Jiang, Zhongyi Hu, Jiao Yuan, Tianli Zhang, Yutian Pan, Mu Xu, Chunsheng Li, Youyou Zhang, Lin Zhang, and Xiaowen Hu
8. RNA-Sequencing Analysis Pipeline for Prognostic Marker Identification in Cancer
Sudhanshu Shukla and Seema Khadirnaikar
Part III: Metastasis Promotion
9. Correlation of Circulating Tumor Cell Measurements with 3D Quantitative Tumor Characterization to Predict Clinical Outcomes in Cancer
Sweet Ping Ng, Clifton David Fuller, and Heath Devin Skinner
10. Extracellular Vesicles and Their Roles in Cancer Progression
Wen-Hsuan Chang, Richard A. Cerione, and Marc A. Antonyak
11. Analysis of Tumor-Derived Exosomes by Nanoscale Flow Cytometry
Cynthia Lopez-Pacheco, Andrea Bedoya-Lopez, Roxana Olguin-Alor, and Gloria Soldevila
12. In Vitro Models for Studying Tumor Progression
Juan Carlos Gonzalez-Orozco, Saul Gaona-Dominguez, and Ignacio Camacho-Arroyo
13. Assessment of Cell Cycle in Primitive Chronic Myeloid Leukemia Cells by Flow Cytometry after Co-Culture with Endothelial Cells
Patricia Torres-Barrera, Mireya Ramirez-Florencio, and Antonieta Chavez-Gonzalez
Part IV: New Technologies in the Study of Cancer
14. Chimeric Antigen Receptor (CAR) T-Cell Therapy for Cancer: Challenges and Opportunities
Arimelek Cortes-Hernandez, Evelyn Alvarez-Salazar, and Gloria Soldevila
15. Cell-Internalization SELEX of RNA Aptamers as a Starting Point for Prostate Cancer Research
Mauricio Rodriguez-Dorantes, Sergio Alberto Cortes-Ramirez, Jenie Marian Cruz-Burgos, Juan Pablo Reyes-Grajeda, Alberto Losada-Garcia, Vanessa Gonzalez-Covarrubias, and Carlos David Cruz-Hernandez
16. Generation of Functional Genetic Study Models in Zebrafish Using CRISPR-Cas9
Francisco Carmona-Aldana, Hober N Nunez-Martinez, Carlos A Peralta-Alvarez, Gustavo Tapia-Urzua, and Felix Recillas-Targa
17. Developing a Model for a siRNA Delivery System by Cancer Implantation into Zebrafish Circulation
Yasuhito Shimada and Mai Hazekawa
18. Fabrication of Adhesive Substrate for Incorporating Hydrogels to Investigate the Influence of Stiffness on Cancer Cell Behavior
Genaro Vazquez-Victorio, Adriana Rodriguez-Hernandez, Mariel Cano-Jorge, Ana Ximena Monroy-Romero, Marina Macias-Silva, and Mathieu Hautefeuille
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