Testicular germ cell tumors : methods and protocols
Author(s)
Bibliographic Information
Testicular germ cell tumors : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 2195）（Springer protocols）
Humana Press, c2021
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Note
Includes bibliographical references and index
Description and Table of Contents
Description
This volume presents the latest techniques used to study germ cell tumors (GCTs), including histopathology; in vitro and in vivo techniques for investigating GCT biology; novel methods for detecting minimal residual diseases; and sequencing considerations as they apply to GCTs. Chapters cover topics such as clinical applications of immunohistochemistry in GCTs in men; fluorescence in situ hybridization (FISH) detection of chromosomal 12p anomalies in testicular GCTs; evaluation of chemotherapeutic drugs for treatment of (cisplatin-resistant) germ cell cancer cell lines; targeted methylation analyses; and circulating microRNA panel for malignant GCT diagnosis and monitoring. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Testicular Germ Cell Tumors: Methods and Protocols provides valuable information that is useful for both novice and expert GCT researchers interested in learning more about this field.
Table of Contents
Preface...Table of Contents...Contributing Authors...
1. Basic Histopathologic Assessment of Germ Cell Tumors for Clinic and ResearchPayal Kapur and Dinesh Rakheja
2. Clinical Applications of Immunohistochemistry in Germ Cell Tumors in MenFranto Francis and Ming Zhou
3. Molecular Characterization of Testicular Germ Cell Tumors Using Tissue MicrodissectionLiang Cheng, Steven A. Mann, Antonio Lopez-Beltran, Michal Chovanec, Matteo Santoni, Mingsheng Wang, Costantine Albany, Nabil Adra, Darrell D. Davidson, Alessia Cimadamore, Rodolfo Montironi, and Shaobo Zhang
4. Fluorescence In Situ Hybridization (FISH) Detection of Chromosomal 12p Anomalies in Testicular Germ Cell TumorsLiang Cheng, Darrell D. Davidson, Rodolfo Montironi, Mingsheng Wang, Antonio Lopez-Beltran, Timothy A. Masterson, Costantine Albany, and Shaobo Zhang
5. Germ Cell Tumor Cell Culture Techniques John Lafin, James F. Amatruda, and Aditya Bagrodia
6. Three-Dimensional Cultivation of Germ Cell Cancer Cell Lines as Hanging DropsMargaretha A. Skowron, Meike M. Watolla, and Daniel Nettersheim
7. Cultivation of Testicular Germ Cell Cancer Cell Lines and Establishment of Gene-Edited Subclones using CRISPR/Cas9Sina Jostes, Daniel Nettersheim, Simon Schneider, and Hubert Schorle
8. Evaluation of Chemotherapeutic Drugs for Treatment of (Cisplatin-Resistant) Germ Cell Cancer Cell LinesMargaretha A. Skowron, Michele J. Hoffmann, Meike M. Watolla, and Daniel Nettersheim
9. Assessing Homologous Recombination and Interstrand Crosslink Repair in Embryonal Carcinoma Testicular Germ Cell Tumor Cell LinesFrancesca Cavallo, Cinzia Caggiano, Maria Jasin, and Marco Barchi
10. Production and Analysis of Human Primordial Germ Cell-Like CellsShino Mitsunaga, Keiko Shioda, Jacob H. Hanna, Kurt J. Isselbacher, and Toshi Shioda
11. A Genetically Engineered Mouse Model of Malignant Testicular Germ Cell TumorsAmy M. Lyndaker, Timothy M. Pierpont, Amanda R. Loehr, and Robert S. Weiss
12. Targeted Methylation Analyses: From Bisulfite Treatment to Quantification Joao Lobo, Ad J. M. Gillis, and Leendert H. J. Looijenga
13. Integrated Analysis of Germ Cell TumorsAlok Tewari and Eliezer Van Allen
14. Use of Genome-Wide Association Studies to Evaluate Genetic Predisposition to Testicular Germ Cell TumorsAnthony J. Hooten, Erica Langer, and Jenny N. Poynter
15. A Circulating MicroRNA Panel for Malignant Germ Cell Tumor Diagnosis and MonitoringMatthew J. Murray, Cinzia G. Scarpini, and Nicholas Coleman
16. Detection of Circulating Tumor Cells (CTCs) in Patients with Testicular Germ Cell Tumors Paulina Nastaly, Friedemann Honecker, Klaus Pantel, and Sabine Riethdorf
17. Developing and Using a Data Commons for Understanding the Molecular Characteristics of Germ Cell TumorsBo Ci, Shin-Yi Lin, Bo Yao, Danni Luo, Lin Xu, Mark Krailo, Matthew Murray, James F. Amatruda, A. Lindsay Frazier, and Yang Xie
Subject Index List...
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