Molecular dermatology : methods and protocols
著者
書誌事項
Molecular dermatology : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, v. 2154）（Springer protocols）
Humana Press : Springer, c2020
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
This volume delivers the most important laboratory techniques that underlie dermatological investigation, including in situ, in vitro, ex vivo, and in vivo models of skin, its appendages (hair follicles, sebaceous and sweat glands), and immune cells. Also included are unique techniques that unravel epigenetic mechanisms in skin and hair follicle cells, such as gene organization in the nucleus and gene expression regulation by the non-coding transcriptome, which are important recent developments in investigative dermatology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Molecular Dermatology: Methods and Protocols serves as an essential resource for researchers working in the areas of fundamental as well as applied dermatological science.
目次
1. Isolation of Epidermal Keratinocytes from Human Skin: The Scratch-Wound Assay for Assessment of Epidermal Keratinocyte Migration
Irene Castellano-Pellicena and M. Julie Thornton
2. Isolation of Different Dermal Fibroblast Populations from the Skin and the Hair Follicle
Rachael Williams and M. Julie Thornton
3. Isolation of Epidermal and Hair Follicle Melanocytes
Richard Baker and M. Julie Thornton
4. Isolation and Culture of Human Skin Mast Cells
Hanna Siiskonen and Joerg Scheffel
5. Organotypic 3D Skin Models: Human Epidermal Equivalent Cultures from Primary Keratinocytes and Immortalized Keratinocyte Cell Lines
Gijs Rikken, Hanna Niehues, and Ellen H. van den Bogaard
6. Purification of Extracellular Microvesicles Secreted by Dermal Fibroblasts
Isabelle Petit, Ayelet Levy, and Daniel Aberdam
7. A Method to Investigate the Epidermal Permeability Barrier In Vitro
Yoshikazu Uchida and Anna Celli
8. Isolating Dermal Papilla Cells from Human Hair Follicles Using Microdissection and Enzyme Digestion
Summik Limbu and Claire A. Higgins
9. Methods to Study Human Hair Follicle Growth Ex Vivo: Human Microdissected Hair Follicle and Human Full Thickness Skin Organ Culture
Janin Lehmann, Jennifer Gherardini, and Marta Bertolini
10. Non-Surgical Induction of Alopecia Areata in C3H/HeJ Mice via Adoptive Transfer of Cultured Lymphoid Cells
Eddy H.C. Wang and Kevin J. McElwee
11. Collapse and Restoration of Hair Follicle Immune Privilege Ex Vivo: A Model for Alopecia Areata
Markus Fehrholz and Marta Bertolini
12. Experimentally-Induced Epithelial-Mesenchymal Transition of Human Hair Follicle Stem Cells as a Model of Scarring Alopecia Ex Vivo
Ilaria Piccini and Marta Bertolini
13. Generation of Hair Follicle Germs in Vitro Using Human Postnatal Skin Cells
Ekaterina P. Kalabusheva and Ekaterina A. Vorotelyak
14. Method for Human Eccrine Sweat Gland Isolation from the Scalp by Means of the Micro-Punch Technique and Vital Dyes
Francisco Jimenez
15. Identification of Long Non-Coding RNA by In Situ Hybridization Approaches
Mara Mancini, Anna Maria Lena, and Eleonora Candi
16. Detection of MicroRNAs by In Situ Hybridization in Skin
Maximilian E. Pickup and Mohammed I. Ahmed
17. Chromatin Immunoprecipitation of Low Number of FACS-Purified Epidermal Cells
Carmit Bar, V. Julian Valdes, and Elena Ezhkova
18. 3D-FISH Analysis of the Spatial Genome Organization in Skin Cells In Situ
Andrei N. Mardaryev and Michael Y. Fessing
19. Method to Study Skin Cancer: Two-Stage Chemically-Induced Carcinogenesis in Mouse Skin
Andrei N. Mardaryev
20. Investigation of Skin Wound Healing Using a Mouse Model
Jianmin Wu and Ning Xu Landen
21. Human Wound Healing Ex Vivo Model with Focus on Molecular Markers
Jennifer Gherardini, Max van Lessen, Ilaria Piccini, Janin Lehmann, and Marta Bertolini
22. Method for Investigation of Photobiological Effects of Light on Human Skin Cells Mediated by Low Doses of Light
Charles Mignon and Natallia E. Uzunbajakava
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