The oral microbiome : methods and protocols

Author(s)

    • Adami, Guy R.

Bibliographic Information

The oral microbiome : methods and protocols

edited by Guy R. Adami

（Methods in molecular biology, 2327）

Springer, 2021

  • hbk.

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Description and Table of Contents

Description

This volume discusses the oral microbiome, and oral and systemic health. The chapters in this book cover topics such as analytical techniques for identifying and measuring oral bacteria; strategies for controlling common sources of variability in oral microbiome methods for viral bacterial and fungal analysis; ways to study oral DNA and RNA samples to identify molecular pathways to disease; approaches to functional assays for oral bacteriophage, antibiotic purging of systemic bacteria; and metaproteomic analysis of various oral samples. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, The Oral Microbiome: Methods and Protocols is a valuable resource for any scientist or researcher looking to further study this exciting and developing field.

Table of Contents

Preface.. Table of Contents... Contributing Authors... 1. Increasing Reproducibility in Oral Microbiome Research Divya Gopinath and Rohit Kunnath Menon 2. Oral Sampling Techniques Heba Hussein 3. Functional Metagenomic Screening for Antimicrobial Resistance in the Oral Microbiome Supathep Tansirichaiya, Liam J. Reynolds, and Adam P. Roberts 4. Isolation and Functional Characterization of Fusobacterium nucleatum Bacteriophage Mwila Kabwe, Teagan Brown, Heng Ku, Stuart Dashper, and Joseph Tucci 5. Comparison of Microbiome in Stimulated Saliva in Edentulous and Dentate Subjects Guy R. Adami, Michael J. Ang, and Elissa M. Kim 6. Host DNA Depletion in Saliva Samples for Improved Shotgun Metagenomics Clarisse Marotz, Cristal Zuniga, Livia Zaramela, Rob Knight, and Karsten Zengler 7. A Standardized Approach for Shotgun Metagenomic Analysis of Ancient Dental Calculus Nicole E. Moore and Laura S. Weyrich 8. Whole-Genome Sequencing of Pathogens in Saliva: A Target-Enrichment Approach for SARS-CoV-2 David J. Speicher, Jalees A. Nasir, Peng Zhou, and Danielle E. Anderson 9. Assessing the Relationship Between Nitrate-Reducing Capacity of the Oral Microbiome and Systemic Outcomes Charlene E. Goh, Bruno Bohn, and Ryan T. Demmer 10. Identification of Oral Bacterial Biosynthetic Gene Clusters Associated with Caries Jonathon L. Baker and Anna Edlund 11. Usage of Metatranscriptomics to Understand Oral Disease Takayasu Watanabe 12. Noninvasive Acquisition of Oral Mucosal Epithelial miRNA and Bacteria DNA/RNA from a Single Site Guy R. Adami 13. Bottom-Up Community Proteome Analysis of Saliva Samples and Tongue Swabs by Data-Dependent Acquisition Nano LC-MS/MS Mass Spectrometry Alexander Rabe, Manuela Gesell Salazar, and Uwe Voelker 14. Strain Level Profiling of Oral Microbiota with Targeted Sequencing Chiranjit Mukherjee and Eugene J. Leys 15. Profiling the Human Oral Mycobiome in Tissue and Saliva Using ITS2 DNA Metabarcoding Compared to a Fungal Specific Database David J. Speicher and Ramy K. Aziz 16. Measuring Effects of Dietary Fiber on the Murine Oral Microbiome with Enrichment of 16S rDNA Prior to Amplicon Synthesis Lea M. Sedghi, Stefan J. Green, and Craig D. Byron 17. Antibiotic Conditioning and Single Gavage Allows Stable Engraftment of Human Microbiota in Mice Zhigang Zhu, Thomas Kaiser, and Christopher Staley Subject Index List...

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