Intestinal stem cells : methods and protocols

著者

• Ordóñez-Morán, Paloma

書誌事項

Intestinal stem cells : methods and protocols

edited by Paloma Ordóñez-Morán

（Methods in molecular biology / John M. Walker, series editor, 2171）（Springer protocols）

Humana Press, c2020

注記

"Springer Science+Business Media, LLC, part of Springer Nature 2020"--T.p. verso

Includes bibliographical references and index

内容説明・目次

内容説明

This detailed book encapsulates the most up-to-date methods of the intestinal stem cell field and provides guidance on a variety of techniques for studying intestinal stem cells properties. Beginning with a section on in vitro techniques to study different aspects of the intestinal stem cell functions by innovative imaging and functional assays, the volume continues with chapters detailing the single-cell transcriptional profiling method, the isolation of intestinal crypts to generate and establish 3D organoids, as well as different animal models of gastrointestinal cancer and examples of the use of in vivo methods for studying intestinal tumor-initiating cells or cancer stem cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and state-of-the-art, Intestinal Stem Cells: Methods and Protocols aims to provide comprehensive and easy to follow protocols designed to be helpful to both seasoned researchers and newcomers to this dynamic field.

目次

Part I: Characterization, Imaging, and Functional Assays 1. Identification and Isolation of Human LGR5+ Cells Using an Antibody-Based Strategy Michael K. Dame, Sha Huang, Durga Attili, Jason R Spence, and Justin A. Colacino 2. Immune-Mediated Specific Depletion of Intestinal Stem Cells Stephen E. Sherman and Judith Agudo 3. Analysis of Aged Dysfunctional Intestinal Stem Cells Kodandaramireddy Nalapareddy and Hartmut Geiger 4. Strategies for Measuring Induction of Fatty Acid Oxidation in Intestinal Stem and Progenitor Cells Chia-Wei Cheng, Omer H. Yilmaz, and Maria M. Mihaylova 5. Visualization of Stem Cell Niche by the Fluorescence Lifetime Imaging Microscopy Irina A. Okkelman, Jens Puschhof, Dmitri B. Papkovsky, and Ruslan I. Dmitriev 6. Generation and Quantitative Imaging of Enteroid Monolayers Laura E. Sanman, Ina W. Chen, Jake M. Bieber, Curtis A. Thorne, Lani F. Wu, and Steven J. Altschuler 7. Autophagy Detection in Intestinal Stem Cells Jumpei Asano, Taku Sato, and Toshiaki Ohteki Part II: Single-Cell Transcriptional Profiling of the Small Intestinal Epithelium 8. Single-Cell Transcriptional Profiling of the Intestinal Epithelium Claudia Capdevila, Ruben I. Calderon, Erin C. Bush, Kismet Sheldon-Collins, Peter A. Sims, and Kelley S. Yan 9. Single-Cell Studies of Intestinal Stem Cell Heterogeneity during Homeostasis and Regeneration Maxim Norkin, Claudia Capdevila, Ruben I. Calderon, Tianhong Su, Maria Trifas, Paloma Ordonez-Moran, and Kelley S. Yan Part III: Organoids and Applications 10. Large-Scale Production of Recombinant Noggin and R-Spondin1 Proteins Required for the Maintenance of Stem Cells in Intestinal Organoid Cultures David L. Hacker and Paloma Ordonez-Moran 11. Primary Intestinal Epithelial Organoid Culture Tomohiro Mizutani and Hans Clevers 12. In Vivo Human PSC-Derived Intestinal Organoids to Study Stem Cell Maintenance Simon Vales, Holly M. Poling, Nambirajan Sundaram, Michael A. Helmrath, and Maxime M. Mahe 13. Generation of Knock-Out Gene-Edited Human Intestinal Organoids Chathruckan Rajendra, Tomas Wald, Kevin Barber, Jason R. Spence, Faranak Fattahi, and Ophir D. Klein 14. Direct Lineage Reprogramming of Mouse Fibroblasts to Acquire the Identity of Fetal Intestine-Derived Progenitor Cells Shizuka Miura and Atsushi Suzuki 15. Single Molecule RNA FISH in Whole-Mount Organoids Costanza Borrelli and Andreas E. Moor 16. Specific Gene Expression in Lgr5+ Stem Cells by Using Cre-Lox Recombination Pierre Dessen, Joerg Huelsken, and Paloma Ordonez-Moran 17. Generating and Utilizing Murine Cas9-Expressing Intestinal Organoids for Large Scale Knockout Genetic Screening Hossein Kashfi, Nicholas Jinks, and Abdolrahman Shams Nateri Part IV: In Vivo Models 18. Mouse Model for Sporadic Mutation of Target Alleles to Understand Tumor Initiation and Progression and Stem Cell Dynamics Theresa Nguyen, Elise Manalo, Taryn Kawashima, and Jared M. Fischer 19. Hemagglutinating Virus of Japan Envelop (HVJ-E)-Guided Gene Transfer to the Intestinal Epithelium Masamichi Imajo 20. An Intrasplenic Injection Model for the Study of Cancer Stem Cells Seeding Capacity Caroline Dafflon, Albert Santamaria-Martinez, and Paloma Ordonez-Moran 21. Organoid Derivation and Orthotopic Xenotransplantation for Studying Human Intestinal Stem Cell Dynamics Shinya Sugimoto, Masayuki Fujii, and Toshiro Sato 22. Advanced Colorectal Cancer Orthotopic Patient-Derived Xenograft Models for Cancer and Stem Cell Research Irene Chicote, Juan Antonio Camara, and Hector G. Palmer 23. Modeling Colorectal Cancer Progression through Orthotopic Implantation of Organoids Felipe de Sousa e Melo, Jonathan M. Harnoss, Noelyn Kljavin, Ryan Scott, Catherine Sohn, Kevin G. Leong, and Frederic J. de Sauvage

「Nielsen BookData」 より

詳細情報

• NII書誌ID(NCID): BC09658967
• ISBN:
  • 9781071607466
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: New York
• ページ数/冊数: xv, 350 p.
• 大きさ: 26 cm
• 分類:
  • DC23 : 616.02/774
• 件名:
  • LCSH : Stem cells -- Laboratory manuals
  • LCSH : Intestines -- Molecular aspects
  • MESH : Stem Cells
• 親書誌ID:
  • BA10794673
  • BA88873632