Intestinal stem cells : methods and protocols
Author(s)
Bibliographic Information
Intestinal stem cells : methods and protocols
(Methods in molecular biology / John M. Walker, series editor, 2171)(Springer protocols)
Humana Press, c2020
Available at 1 libraries
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Note
"Springer Science+Business Media, LLC, part of Springer Nature 2020"--T.p. verso
Includes bibliographical references and index
Description and Table of Contents
Description
This detailed book encapsulates the most up-to-date methods of the intestinal stem cell field and provides guidance on a variety of techniques for studying intestinal stem cells properties. Beginning with a section on in vitro techniques to study different aspects of the intestinal stem cell functions by innovative imaging and functional assays, the volume continues with chapters detailing the single-cell transcriptional profiling method, the isolation of intestinal crypts to generate and establish 3D organoids, as well as different animal models of gastrointestinal cancer and examples of the use of in vivo methods for studying intestinal tumor-initiating cells or cancer stem cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and state-of-the-art, Intestinal Stem Cells: Methods and Protocols aims to provide comprehensive and easy to follow protocols designed to be helpful to both seasoned researchers and newcomers to this dynamic field.
Table of Contents
Part I: Characterization, Imaging, and Functional Assays
1. Identification and Isolation of Human LGR5+ Cells Using an Antibody-Based Strategy
Michael K. Dame, Sha Huang, Durga Attili, Jason R Spence, and Justin A. Colacino
2. Immune-Mediated Specific Depletion of Intestinal Stem Cells
Stephen E. Sherman and Judith Agudo
3. Analysis of Aged Dysfunctional Intestinal Stem Cells
Kodandaramireddy Nalapareddy and Hartmut Geiger
4. Strategies for Measuring Induction of Fatty Acid Oxidation in Intestinal Stem and Progenitor Cells
Chia-Wei Cheng, Omer H. Yilmaz, and Maria M. Mihaylova
5. Visualization of Stem Cell Niche by the Fluorescence Lifetime Imaging Microscopy
Irina A. Okkelman, Jens Puschhof, Dmitri B. Papkovsky, and Ruslan I. Dmitriev
6. Generation and Quantitative Imaging of Enteroid Monolayers
Laura E. Sanman, Ina W. Chen, Jake M. Bieber, Curtis A. Thorne, Lani F. Wu, and Steven J. Altschuler
7. Autophagy Detection in Intestinal Stem Cells
Jumpei Asano, Taku Sato, and Toshiaki Ohteki
Part II: Single-Cell Transcriptional Profiling of the Small Intestinal Epithelium
8. Single-Cell Transcriptional Profiling of the Intestinal Epithelium
Claudia Capdevila, Ruben I. Calderon, Erin C. Bush, Kismet Sheldon-Collins, Peter A. Sims, and Kelley S. Yan
9. Single-Cell Studies of Intestinal Stem Cell Heterogeneity during Homeostasis and Regeneration
Maxim Norkin, Claudia Capdevila, Ruben I. Calderon, Tianhong Su, Maria Trifas, Paloma Ordonez-Moran, and Kelley S. Yan
Part III: Organoids and Applications
10. Large-Scale Production of Recombinant Noggin and R-Spondin1 Proteins Required for the Maintenance of Stem Cells in Intestinal Organoid Cultures
David L. Hacker and Paloma Ordonez-Moran
11. Primary Intestinal Epithelial Organoid Culture
Tomohiro Mizutani and Hans Clevers
12. In Vivo Human PSC-Derived Intestinal Organoids to Study Stem Cell Maintenance
Simon Vales, Holly M. Poling, Nambirajan Sundaram, Michael A. Helmrath, and Maxime M. Mahe
13. Generation of Knock-Out Gene-Edited Human Intestinal Organoids
Chathruckan Rajendra, Tomas Wald, Kevin Barber, Jason R. Spence, Faranak Fattahi, and Ophir D. Klein
14. Direct Lineage Reprogramming of Mouse Fibroblasts to Acquire the Identity of Fetal Intestine-Derived Progenitor Cells
Shizuka Miura and Atsushi Suzuki
15. Single Molecule RNA FISH in Whole-Mount Organoids
Costanza Borrelli and Andreas E. Moor
16. Specific Gene Expression in Lgr5+ Stem Cells by Using Cre-Lox Recombination
Pierre Dessen, Joerg Huelsken, and Paloma Ordonez-Moran
17. Generating and Utilizing Murine Cas9-Expressing Intestinal Organoids for Large Scale Knockout Genetic Screening
Hossein Kashfi, Nicholas Jinks, and Abdolrahman Shams Nateri
Part IV: In Vivo Models
18. Mouse Model for Sporadic Mutation of Target Alleles to Understand Tumor Initiation and Progression and Stem Cell Dynamics
Theresa Nguyen, Elise Manalo, Taryn Kawashima, and Jared M. Fischer
19. Hemagglutinating Virus of Japan Envelop (HVJ-E)-Guided Gene Transfer to the Intestinal Epithelium
Masamichi Imajo
20. An Intrasplenic Injection Model for the Study of Cancer Stem Cells Seeding Capacity
Caroline Dafflon, Albert Santamaria-Martinez, and Paloma Ordonez-Moran
21. Organoid Derivation and Orthotopic Xenotransplantation for Studying Human Intestinal Stem Cell Dynamics
Shinya Sugimoto, Masayuki Fujii, and Toshiro Sato
22. Advanced Colorectal Cancer Orthotopic Patient-Derived Xenograft Models for Cancer and Stem Cell Research
Irene Chicote, Juan Antonio Camara, and Hector G. Palmer
23. Modeling Colorectal Cancer Progression through Orthotopic Implantation of Organoids
Felipe de Sousa e Melo, Jonathan M. Harnoss, Noelyn Kljavin, Ryan Scott, Catherine Sohn, Kevin G. Leong, and Frederic J. de Sauvage
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