Small non-coding RNAs : methods and protocols

著者

書誌事項

Small non-coding RNAs : methods and protocols

edited by Mathieu Rederstorff

（Methods in molecular biology / John M. Walker, series editor, 2300）（Springer protocols）

Humana Press, c2021

2nd ed

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注記

"Springer Science+Business Media, LLC, part of Springer Nature 2015,2021"--T.p. verso

Includes bibliographical references and index

内容説明・目次

内容説明

This second edition provides new and updated chapters detailing methods tackling all aspects of small non-coding RNAs biology. Chapters guide readers through customized dedicated protocols and technologies that will be of valuable help decipher the numerous functions of small non-coding RNAs. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls. Instructive and practical, Small Non-Coding RNAs: Methods and Protocols, Second Edition aims to be a valuable help and of great interest to a large number of researchers working in the field of RNA or willing to develop RNA studies.

目次

PART I INTRODUCTION TO SMALL NON-CODING RNAS 1 Regulatory Non-coding RNAs: An Overview Camille Virciglio, Yoann Abel, and Mathieu Rederstorff 2 RNA Precipitation Valerie Bourguignon-Igel, Yoann Abel, and Mathieu Rederstorff 3 Quantitative and Qualitative Assessment of Small RNA Preparations Virginie Marchand, Lilia Ayadi, Valerie Bourguignon-Igel, and Yuri Motorin 4 Non-coding RNA Silencing in Mammalian Cells by Antisense LNA GapmeRs Transfection Charbel Alfeghaly, Christelle Aigueperse, Sylvain Maenner, and Isabelle Behm-Ansmant PART II DETECTION OF SMALL NON-CODING RNAS 5 Northern Blot Detection of Tiny RNAs Jana C. Wiegard, M. Amri C. Schluter, Olga Y. Burenina, Elena A. Kubareva, Gabriele Klug, Arnold Grunweller, and Roland K. Hartmann 6 Stem-loop qRT-PCR Based Quantification of miRNAs Yoann Abel and Mathieu Rederstorff 7 Detection and Labeling of Small Non-coding RNAs by Splinted Ligation Marlene Vayssieres, Gabrielle Bourgeois, Florian Chardon, Anne-Sophie Tillault, and Magali Blaud 8 Fluorescence In Situ Hybridization Of Small Non-coding RNAs Valentin Vautrot, Geraud Heckler, Christelle Aigueperse, and Isabelle Behm-Ansmant PART III FUNCTIONAL ANALYSIS OF SMALL NON-CODING RNAS 9 Using native RIP, UV-CLIP or fCLIP to Address Protein-RNA Interactions In Vivo Layea Baldini and Stephane Labialle 10 Purification of RiboNucleoProtein Particles by MS2-MBP Affinity Chromatography Aymeric Sanchez and Sylvain Maenner 11 Study of genome-wide occupancy of long non-coding RNAs using Chromatin Isolation by RNA Purification (ChIRP) Charbel Alfeghaly, Isabelle Behm-Ansmant, and Sylvain Maenner 12 Gene Reporter Assays to Study miRNA Function Yoann Abel and Mathieu Rederstorff 13 CRISPR/Cas9 System to Knock-Down microRNA In Vitro and In Vivo Bin Yi, Kristina Larter, and Yaguang Xi PART IV HIGH-THROUGHPUT ANALYSIS OF SMALL NON-CODING RNAS 14 Microarray Analysis of Whole-Transcriptome RNAs including Non-Coding RNAs Quentin Thuillier and Isabelle Behm-Ansmant 15 Isolation, Extraction, and Deep-Sequencing Analysis Of Extracellular RNAs (exRNAs) from Human Plasma Virginie Marchand, Adeline Galvanin, and Yuri Motorin 16 Analysis of the RNA and protein complexome by Grad-seq Jens Hoer and Joerg Vogel 17 IVC-Seq: A Method for Ultrahigh-throughput Development and Functional Characterization of Small RNAs Farah Bouhedda, Roger Cubi, Stephanie Baudrey and Michael Ryckelynck PART V STRUCTURAL APPROACHES 18 RNA Secondary Structure Study by Chemical Probing Methods using DMS and CMCT Fatima Alghoul, Gilbert Eriani, and Franck Martin 19 Application of NMR Spectroscopy to Determine the 3D structure of Small Non-coding RNAs Marie-Eve Chagot, Marc Quinternet, Xavier Manival, and Isabelle Lebars

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