CiNii 図書 - Atherosclerosis : methods and protocols

This volume provides detailed, up-to-date methods used in research on Atherosclerosis. Chapters guide readers through an overview of the pathogenesis of atherosclerosis and model systems together with in vitro, ex vivo, in vivo and emerging methods in atherosclerosis research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Atherosclerosis: Methods and Protocols serves as an invaluable resource for those engaging in research on atherosclerosis and cardiovascular disease, as well as for researchers who are new to the field and students at both the undergraduate and postgraduate level.

PART I: Atherosclerosis: Pathogenesis, model systems and emerging concepts. 1. Atherosclerosis: Pathogenesis and key cellular processes, current and emerging therapies, key challenges and future research directions Yee-Hung Chan and Dipak P. Ramji 2. Key roles of inflammation in atherosclerosis: Mediators involved in orchestrating the inflammatory response and its resolution in the disease along with therapeutic avenues targeting inflammation Yee-Hung Chan and Dipak P. Ramji 3. Survey of in vitro model systems for investigation of key cellular processes associated with atherosclerosis Dipak P. Ramji, Alaa Ismail, Jing Chen, Fahad Alradi, and Sulaiman Al Alawi 4. Survey of approaches for investigation of atherosclerosis in vivo Dipak P. Ramji, Yee-Hung Chan, Alaa Alahmadi, Reem Alotibi, and Nouf Alshehri 5. Exploring the associations between clonal hematopoiesis of indeterminate potential, myeloid malignancy and atherosclerosis Juan Bautista Menendez-Gonzalez, and Neil P. Rodrigues 6. Peptides as therapeutic agents for atherosclerosis C. Roger White, Mayakonda Palgunachari, Paul Wolkowicz, and G. M. Anantharamaiah PART II: In vitro and ex vivo model systems for atherosclerosis research. 7. Isolation and culturing of primary mouse and human macrophages Alba de Juan and Begona Lavin 8. Effective transfection and gene silencing of primary murine macrophages with small interfering RNA Kim van Kuijk, Jan Nagenborg, and Judith C. Sluimer 9. Monitoring celular proliferation, migration and apoptosis associated with atherosclerosis plaques in vitro Kerry S. Wadey, Alexandros Somos, Stephen J. Cross, Lien M. Reolizo, Jason L. Johnson, and Sarah J. George 10. Monocyte adhesion assays for detecting endothelial cell activation in vascular inflammation and aterosclerosis Keman Xu, Fatma Saaoud, Stephanie Yu, Charles Drummer IV, Ying Shao, Yu Sun, Yifan Lu, Jianxin Sun, Jun Yu, Xiaohua Jiang, Hong Wang, and Xiaofeng Yang 11. Chick embryo ex vivo aortic sprouting assays for cardiovascular research Selvaraj Vimalraj and Anuradha Dhanasekaran 12. Purification and analysis of circulating lipid particles Barnaby W. R. Roper, Basmah Al-Sayejh, Ahmed Al-Aufi, Gary A. Cuthbert, Katie Lacey, Shervanthi Homer-Vanniasinkam, Michael A. Harrison, Darren C. Tomlinson, Ramzi Ajjan, and Sreenivasan Ponnambalam 13. Preparation of LDL, oxidation, methods of detection and applications in atherosclerosis research Chandrakala Aluganti Narasimhulu and Sampath Parthasarathy 14. Monitoring modified lipoprotein uptake and macropinocytosis associated with macrophage foam cell formation Alaa A. Alahmadi and Dipak P. Ramji 15. A fluorescent based in vitro method to assess cholesterol efflux. Sara Fernandez-Castillejo, Anna Pedret, Ursula Catalan, and Rosa Sola 16. Monitoring the anisotropy and fluidity of the HDL monolayer as surrogates of HDL functionality Sara Fernandez-Castillejo, Anna Pedret, Ursula Catalan, Claude Motta, and Rosa Sola 17. Assessment of ex-vivo antioxidative potential of murine HDL in atherosclerosis Andrea Rivas-Urbina, Noemi Rotllan, David Santos, Josep Julve, Joan Carles Escola-Gil, and Jose Luis Sanchez-Quesada 18. Dual-fluorescence in vitro efferocytosis assay Arif Yurdagul Jr 19. Investigation of mitochondrial bioenergetic profile and dysfunction in atherosclerosis Yee-Hung Chan, and Dipak P. Ramji 20. Probing inflammasome activation in atherosclerosis Yee-Hung Chan and Dipak P. Ramji 21. Investigation of platelet aggregation in atherosclerosis Ronan Lordan, Alexandros Tsoupras, and Ioannis Zabetakis 22. Extracellular vesicles in atherosclerosis research Jessica O. Williams, Cass Whelan, Jamie Nash, and Philip E. James 23. The use of wire myography to investigate vascular tone and function Kayleigh Griffiths and Melanie Madhani PART III: In vivo model systems for atherosclerosis research. 24. Pig and mouse models of hyperlipidemia and atherosclerosis Godfrey S. Getz and Catherine A. Reardon 25. Use of rabbit models to study atherosclerosis Jianglin Fan, Manabu Nimimi, Yajie Chen, Ritsuko Suzuki, and Enqi Liu 26. Genetically engineered hamster models of dyslipidemia and atherosclerosis Xunde Xian, Yuhui Wang, and George Liu 27. Atherosclerosis induced by adeno-associated virus encoding gain-of-function PCSK9 Martin Maeng Bjorklund, Juan A. Bernal, and Jacob F. Bentzon 28. Monitoring leukocyte migration during atherosclerosis in vivo Keiichiro Matoba 29. Evaluation of plaque burden and lipid content in atherosclerotic plaques Yee-Hung Chan, Alaa Alahmadi, Reem Alotibi, and Dipak P. Ramji 30. Monitoring cellularity and expression of key markers in atherosclerotic plaques. Yee-Hung Chan, Reem Alotibi, Alaa Alahmadi, and Dipak P. Ramji 31. Monitoring cellular proliferation and apoptosis in atherosclerosis plaques and intimal thickenings Kerry S. Wadey, Jason L. Johnson, and Sarah J. George 32. Investigation of atherosclerotic plaque vulnerability Sarah J. George and Jason L. Johnson 33. Use of mouse carotid artery ligation model of intimal thickening to probe vascular smooth muscle cell remodelling and function in atherosclerosis Helen Williams, Bethan A. Brown, Jason L. Johnson, and Sarah J. George 34. Assessing efferocytosis in atherosclerotic lesions in situ Arif Yurdagul Jr 35. Monitoring atheroprotective macrophage cholesterol efflux in vivo Andrea Rivas-Urbina, Noemi Rotllan, David Santos, Josep Julve, Jose Luis Sanchez-Quesada, and Joan Carles Escola-Gil 36. Flow cytometry analysis of hematopoietic stem/progenitor cells and mature blood cell subsets in atherosclerosis Alhomidi Almotiri, Ali Abdelfattah, and Neil P. Rodrigues 37. Whole mount preparation of mouse aorta for confocal microscopy studies of the intima Ana Barettino, Ignacio Benedicto, and Vicente Andres 38. Isolation of mouse aortic RNA for transcriptomics Rosa M. Nevado, Magda R. Hamczyk, and Vicente Andres 39. Redox proteomics analysis of atherosclerotic aortas: Application of the "OxICAT" method Manousos Makridakis and Antonia Vlahou 40. Intravital microscopy in atherosclerosis research Georg Wissmeyer, Mohamad B. Kassab, Yoichiro Kawamura, Aaron D. Aguirre, and Farouc A. Jaffer 41. Localization of long non-coding RNA in formalin-fixed-paraffin embedded vascular tissue using in-situ hybridization Jessica P. Scanlon, Andrew H. Baker, and Judith C. Sluimer PART IV: Emerging techniques in atherosclerosis research. 42. In vivo gene editing in lipid and atherosclerosis research Marco De Giorgi, Kelsey E. Jarrett, Thomas Q. de Aguiar Vallim, and William R. Lagor 43. Laser capture microdissection-based mRNA expression microarrays and single-cell RNA sequencing in atherosclerosis research Xi Zhang, Zhihua Wang, Chuankai Zhang, Yutao Li, Shu Lu, Sabine Steffens, Sarajo Mohanta, Christian Weber, Andreas Habenicht, and Changjun Yin 44. Combined single cell RNA and single cell / T cell receptor sequencing of the arterial wall in atherosclerosis Zhihua Wang, Xi Zhang, Chuankai Zhang, Yutao Li, Shu Lu, Sarajo Mohanta, Christian Weber, Andreas Habenicht, and Changjun Yin 45. Tissue clearing approaches in atherosclerosis Ting Sun, Yuanfang Li, Benjamin Foerstera, Karen Stanic, Shu Lu, Sabine Steffens, Changjun Yin, Ali Erturk, Remco T. A. Megens, Christian Weber, Andreas Habenicht, and Sarajo K. Mohanta 46. Single cell antibody sequencing in atherosclerosis research Ryosuke Saigusa, Christopher P. Durant, Vasantika Suryawanshi, and Klaus Ley 47. Flow cytometry and mass cytometry for measuring the immune cell infiltrate in atherosclerotic arteries Marco Orecchioni, Melissa A. Meyer, Catherine C. Hedrick and Klaus Ley 48. Contrast-enhanced ultrasound molecular imaging in atherosclerosis The Anh Nguyen and Jonathan R. Lindner 49. Nanoprobes for computed tomography and magnetic resonance imaging in atherosclerosis research Ketan B. Ghaghada, Rohan Bhavane, Andrew Badachhape, Eric Tanifum, and Ananth Annapragada 50. Positron emission tomography in atherosclerosis research Anne Roivainen, Mia Stahle, and Antti Saraste 51. Three-dimensional visualization of atherosclerotic vessels by tissue clearing and light sheet fluorescence microscopy Tobias Becher, Dario F. Riascos-Bernal, Jingyi Chi, Paul Cohen, and Nicholas E. S. Sibinga 52. Intravascular fluorescence molecular imaging of atherosclerosis Mohammed M. Chowdhury, Zhonglie Piao, Mazen S. Albaghdadi, Patrick A. Coughlin, James H.F. Rudd, Guillermo J. Tearney, and Farouc A. Jaffer

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Atherosclerosis : methods and protocols

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