Atherosclerosis : methods and protocols
Author(s)
Bibliographic Information
Atherosclerosis : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 2419）（Springer protocols）
Humana Press, c2022
Available at / 2 libraries
-
No Libraries matched.
- Remove all filters.
Note
Includes bibliographical references and index
Description and Table of Contents
Description
This volume provides detailed, up-to-date methods used in research on Atherosclerosis. Chapters guide readers through an overview of the pathogenesis of atherosclerosis and model systems together with in vitro, ex vivo, in vivo and emerging methods in atherosclerosis research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Atherosclerosis: Methods and Protocols serves as an invaluable resource for those engaging in research on atherosclerosis and cardiovascular disease, as well as for researchers who are new to the field and students at both the undergraduate and postgraduate level.
Table of Contents
PART I: Atherosclerosis: Pathogenesis, model systems and emerging concepts.
1. Atherosclerosis: Pathogenesis and key cellular processes, current and emerging therapies, key challenges and future research directions
Yee-Hung Chan and Dipak P. Ramji
2. Key roles of inflammation in atherosclerosis: Mediators involved in orchestrating the inflammatory response and its resolution in the disease along with therapeutic avenues targeting inflammation
Yee-Hung Chan and Dipak P. Ramji
3. Survey of in vitro model systems for investigation of key cellular processes associated with atherosclerosis
Dipak P. Ramji, Alaa Ismail, Jing Chen, Fahad Alradi, and Sulaiman Al Alawi
4. Survey of approaches for investigation of atherosclerosis in vivo
Dipak P. Ramji, Yee-Hung Chan, Alaa Alahmadi, Reem Alotibi, and Nouf Alshehri
5. Exploring the associations between clonal hematopoiesis of indeterminate potential, myeloid malignancy and atherosclerosis
Juan Bautista Menendez-Gonzalez, and Neil P. Rodrigues
6. Peptides as therapeutic agents for atherosclerosis
C. Roger White, Mayakonda Palgunachari, Paul Wolkowicz, and G. M. Anantharamaiah
PART II: In vitro and ex vivo model systems for atherosclerosis research.
7. Isolation and culturing of primary mouse and human macrophages
Alba de Juan and Begona Lavin
8. Effective transfection and gene silencing of primary murine macrophages with small interfering RNA
Kim van Kuijk, Jan Nagenborg, and Judith C. Sluimer
9. Monitoring celular proliferation, migration and apoptosis associated with atherosclerosis plaques in vitro
Kerry S. Wadey, Alexandros Somos, Stephen J. Cross, Lien M. Reolizo, Jason L. Johnson, and Sarah J. George
10. Monocyte adhesion assays for detecting endothelial cell activation in vascular inflammation and aterosclerosis
Keman Xu, Fatma Saaoud, Stephanie Yu, Charles Drummer IV, Ying Shao, Yu Sun, Yifan Lu, Jianxin Sun, Jun Yu, Xiaohua Jiang, Hong Wang, and Xiaofeng Yang
11. Chick embryo ex vivo aortic sprouting assays for cardiovascular research
Selvaraj Vimalraj and Anuradha Dhanasekaran
12. Purification and analysis of circulating lipid particles
Barnaby W. R. Roper, Basmah Al-Sayejh, Ahmed Al-Aufi, Gary A. Cuthbert, Katie Lacey, Shervanthi Homer-Vanniasinkam, Michael A. Harrison, Darren C. Tomlinson, Ramzi Ajjan, and Sreenivasan Ponnambalam
13. Preparation of LDL, oxidation, methods of detection and applications in atherosclerosis research
Chandrakala Aluganti Narasimhulu and Sampath Parthasarathy
14. Monitoring modified lipoprotein uptake and macropinocytosis associated with macrophage foam cell formation
Alaa A. Alahmadi and Dipak P. Ramji
15. A fluorescent based in vitro method to assess cholesterol efflux. Sara Fernandez-Castillejo, Anna Pedret, Ursula Catalan, and Rosa Sola
16. Monitoring the anisotropy and fluidity of the HDL monolayer as
surrogates of HDL functionality
Sara Fernandez-Castillejo, Anna Pedret, Ursula Catalan, Claude Motta, and Rosa Sola
17. Assessment of ex-vivo antioxidative potential of murine HDL in atherosclerosis
Andrea Rivas-Urbina, Noemi Rotllan, David Santos, Josep Julve, Joan Carles Escola-Gil, and Jose Luis Sanchez-Quesada
18. Dual-fluorescence in vitro efferocytosis assay
Arif Yurdagul Jr
19. Investigation of mitochondrial bioenergetic profile and dysfunction in atherosclerosis
Yee-Hung Chan, and Dipak P. Ramji
20. Probing inflammasome activation in atherosclerosis
Yee-Hung Chan and Dipak P. Ramji
21. Investigation of platelet aggregation in atherosclerosis
Ronan Lordan, Alexandros Tsoupras, and Ioannis Zabetakis
22. Extracellular vesicles in atherosclerosis research
Jessica O. Williams, Cass Whelan, Jamie Nash, and Philip E. James
23. The use of wire myography to investigate vascular tone and function
Kayleigh Griffiths and Melanie Madhani
PART III: In vivo model systems for atherosclerosis research.
24. Pig and mouse models of hyperlipidemia and atherosclerosis
Godfrey S. Getz and Catherine A. Reardon
25. Use of rabbit models to study atherosclerosis
Jianglin Fan, Manabu Nimimi, Yajie Chen, Ritsuko Suzuki, and Enqi Liu
26. Genetically engineered hamster models of dyslipidemia and atherosclerosis
Xunde Xian, Yuhui Wang, and George Liu
27. Atherosclerosis induced by adeno-associated virus encoding gain-of-function PCSK9
Martin Maeng Bjorklund, Juan A. Bernal, and Jacob F. Bentzon
28. Monitoring leukocyte migration during atherosclerosis in vivo
Keiichiro Matoba
29. Evaluation of plaque burden and lipid content in atherosclerotic plaques Yee-Hung Chan, Alaa Alahmadi, Reem Alotibi, and Dipak P. Ramji
30. Monitoring cellularity and expression of key markers in atherosclerotic plaques. Yee-Hung Chan, Reem Alotibi, Alaa Alahmadi, and Dipak P. Ramji
31. Monitoring cellular proliferation and apoptosis in atherosclerosis plaques and intimal thickenings
Kerry S. Wadey, Jason L. Johnson, and Sarah J. George
32. Investigation of atherosclerotic plaque vulnerability
Sarah J. George and Jason L. Johnson
33. Use of mouse carotid artery ligation model of intimal thickening to probe vascular smooth muscle cell remodelling and function in atherosclerosis
Helen Williams, Bethan A. Brown, Jason L. Johnson, and Sarah J. George
34. Assessing efferocytosis in atherosclerotic lesions in situ
Arif Yurdagul Jr
35. Monitoring atheroprotective macrophage cholesterol efflux in vivo
Andrea Rivas-Urbina, Noemi Rotllan, David Santos, Josep Julve, Jose Luis Sanchez-Quesada, and Joan Carles Escola-Gil
36. Flow cytometry analysis of hematopoietic stem/progenitor cells and mature blood cell subsets in atherosclerosis
Alhomidi Almotiri, Ali Abdelfattah, and Neil P. Rodrigues
37. Whole mount preparation of mouse aorta for confocal microscopy studies of the intima
Ana Barettino, Ignacio Benedicto, and Vicente Andres
38. Isolation of mouse aortic RNA for transcriptomics
Rosa M. Nevado, Magda R. Hamczyk, and Vicente Andres
39. Redox proteomics analysis of atherosclerotic aortas: Application of the "OxICAT" method
Manousos Makridakis and Antonia Vlahou
40. Intravital microscopy in atherosclerosis research
Georg Wissmeyer, Mohamad B. Kassab, Yoichiro Kawamura, Aaron D. Aguirre, and Farouc A. Jaffer
41. Localization of long non-coding RNA in formalin-fixed-paraffin embedded vascular tissue using in-situ hybridization
Jessica P. Scanlon, Andrew H. Baker, and Judith C. Sluimer
PART IV: Emerging techniques in atherosclerosis research.
42. In vivo gene editing in lipid and atherosclerosis research
Marco De Giorgi, Kelsey E. Jarrett, Thomas Q. de Aguiar Vallim, and William R. Lagor
43. Laser capture microdissection-based mRNA expression microarrays and single-cell RNA sequencing in atherosclerosis research
Xi Zhang, Zhihua Wang, Chuankai Zhang, Yutao Li, Shu Lu, Sabine Steffens, Sarajo Mohanta, Christian Weber, Andreas Habenicht, and Changjun Yin
44. Combined single cell RNA and single cell / T cell receptor sequencing of the arterial wall in atherosclerosis
Zhihua Wang, Xi Zhang, Chuankai Zhang, Yutao Li, Shu Lu, Sarajo Mohanta, Christian Weber, Andreas Habenicht, and Changjun Yin
45. Tissue clearing approaches in atherosclerosis
Ting Sun, Yuanfang Li, Benjamin Foerstera, Karen Stanic, Shu Lu, Sabine Steffens, Changjun Yin, Ali Erturk, Remco T. A. Megens, Christian Weber, Andreas Habenicht, and Sarajo K. Mohanta
46. Single cell antibody sequencing in atherosclerosis research
Ryosuke Saigusa, Christopher P. Durant, Vasantika Suryawanshi, and Klaus Ley
47. Flow cytometry and mass cytometry for measuring the immune cell infiltrate in atherosclerotic arteries
Marco Orecchioni, Melissa A. Meyer, Catherine C. Hedrick and Klaus Ley
48. Contrast-enhanced ultrasound molecular imaging in atherosclerosis
The Anh Nguyen and Jonathan R. Lindner
49. Nanoprobes for computed tomography and magnetic resonance imaging in atherosclerosis research
Ketan B. Ghaghada, Rohan Bhavane, Andrew Badachhape, Eric Tanifum, and Ananth Annapragada
50. Positron emission tomography in atherosclerosis research
Anne Roivainen, Mia Stahle, and Antti Saraste
51. Three-dimensional visualization of atherosclerotic vessels by tissue clearing and light sheet fluorescence microscopy
Tobias Becher, Dario F. Riascos-Bernal, Jingyi Chi, Paul Cohen, and Nicholas E. S. Sibinga
52. Intravascular fluorescence molecular imaging of atherosclerosis
Mohammed M. Chowdhury, Zhonglie Piao, Mazen S. Albaghdadi, Patrick A. Coughlin, James H.F. Rudd, Guillermo J. Tearney, and Farouc A. Jaffer
by "Nielsen BookData"
