Permeability barrier : methods and protocols
著者
書誌事項
Permeability barrier : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 2367）（Springer protocols）
Humana Press, c2021
2nd ed
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
This detailed edition reflects the significant new findings in the components of permeability barriers and how they work in different tissues with a collection of cutting-edge techniques. Chapters explore the formation, maintenance, regulation, and dynamics of permeability barriers in an effort to push the boundaries of the field. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Permeability Barrier: Methods and Protocols, Second Edition serves as an invaluable guide for both experts but novices in the stem cell field and other related areas of research.
目次
1. Use of Ussing Chambers to Measure Paracellular Permeability to Macromolecules in Mouse Intestine
Doriane Aguanno, Barbara Graziela Postal, Veronique Carriere, and Sophie Thenet
2. Rapid Evaluation of Intestinal Paracellular Permeability Using the Human Enterocytic-Like Caco-2/TC7 Cell Line
Barbara Graziela Postal, Doriane Aguanno, Sophie Thenet, and Veronique Carriere
3. Evaluation of Barrier Functions in Human iPSC-Derived Intestinal Epithelium
Shigeru Yamada and Yasunari Kanda
4. Selective Regional Isolation of Brain Microvessels
Fernanda Medina-Flores, Gabriela Hurtado-Alvarado, and Beatriz Gomez-Gonzalez
5. Estimating Brain Permeability Using In Vitro Blood-Brain Barrier Models
Saeideh Nozohouri, Behnam Noorani, Abraham Al-Ahmad, and Thomas J. Abbruscato
6. In Vitro Human Blood-Brain Barrier Model for Drug Permeability Testing
Ece Bayir and Aylin Sendemir
7. Evaluation of Blood-Brain Barrier Integrity Using Vascular Permeability Markers: Evans Blue, Sodium Fluorescein, Albumin-Alexa Fluor Conjugates, and Horseradish Peroxidase
Bulent Ahishali and Mehmet Kaya
8. Blood-Brain Barrier (BBB) Permeability and Transport Measurement In Vitro and In Vivo
Bingmei M. Fu, Zhen Zhao, and Donghui Zhu
9. Assessment of Blood-Brain Barrier Permeability Using Miniaturized Fluorescence Microscopy in Freely-Moving Rats
Jeffrey L. Barr, G. Cristina Brailoiu, Ellen M. Unterwald, and Eugen Brailoiu
10. Measurement of Lung Vessel and Epithelial Permeability In Vivo with Evans Blue
Prestina Smith, Lauren A. Jeffers, and Michael Koval
11. Measurement of Airway Epithelial Permeability: Methods and Protocols
Hasan Yuksel and Merve OEcalan
12. Vascular Permeability Assays In Vivo
Mir S. Adil and Payaningal R. Somanath
13. Endothelial Permeability Assays In Vitro
Mir S. Adil and Payaningal R. Somanath
14. Mapping Receptor Antibody Endocytosis and Trafficking in Brain Endothelial Cells
Mikkel R. Holst, Simone S.E. Nielsen, and Morten S. Nielsen
15. An In Vitro Assay to Monitor Sertoli Cell Blood-Testis Barrier (BTB) Integrity
Siwen Wu, Lingling Wang, Elizabeth I. Tang, Junlu Wang, and C. Yan Cheng
16. Ussing Chamber Methods to Study the Esophageal Epithelial Barrier
Solange M. Abdulnour-Nakhoul and Nazih L. Nakhoul
17. A Simple Method to Test Mechanical Strain on Epithelial Cell Monolayers Using a 3D-Printed Stretcher
Amanda C. Daulagala, John Yost, Amirreza Yeganegi, William J. Richardson, Michael J. Yost, and Antonis Kourtidis
18. Differentiating between Tight Junction-Dependent and Tight Junction-Independent Intestinal Barrier Loss In Vivo
Sandra D. Chanez-Paredes, Shabnam Abtahi, Wei-Ting Kuo, and Jerrold R. Turner
19. Establishment of Intestinal Epithelial Cell Monolayers and Their Use in Calcium Switch Assay for Assessment of Intestinal Tight Junction Assembly
Pawin Pongkorpsakol, Wilasinee Satianrapapong, Preedajit Wongkrasant, Peter R. Steinhagen, Nuttha Tuangkijkul, Nutthapoom Pathomthongtaweechai, and Chatchai Muanprasat
20. A Method to Prepare a Bioprobe for Regulatory Science of the Drug Delivery System to the Brain: An Angulin Binder to Modulate Tricellular Tight Junction-Seal
Keisuke Tachibana and Masuo Kondoh
21. dSTORM Imaging and Analysis of Desmosome Architecture
Reena R. Beggs, William F. Dean, and Alexa L. Mattheyses
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