Single-domain antibodies : methods and protocols
著者
書誌事項
Single-domain antibodies : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 2446）（Springer protocols）
Humana Press, c2022
- hbk.
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内容説明・目次
内容説明
This volume covers current and emerging techniques for studying single-domain antibodies (sdAbs). Chapters guide readers through the biology and immunology of sdAbs in camelids and sharks, isolation of sdAbs, protein engineering approaches to optimize the solubility, stability, valency and antigen binding affinity of sdAbs, and specialized applications of sdAbs. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
Authoritative and cutting-edge, Single-Domain Antibodies: Methods and Protocols aims to be a useful, practical guide to help researchers further their studies in this field.
目次
Part I: Introduction to Single-Domain Antibodies
1. Nanobodies: From Serendipitous Discovery of Heavy Chain-Only Antibodies in Camelids to a Wide Range of Useful Applications
Fangling Ji, Jun Ren, Cecile Vincke, Lingyun Jia, and Serge Muyldermans
2. Overview, Generation, and Significance of Variable New Antigen Receptors (VNARs) as a Platform for Drug and Diagnostic Development
Samata S. Pandey, Marina Kovaleva, Caroline J. Barelle, and Obinna C. Ubah
Part II: Isolation of Single-Domain Antibodies
3. Llama DNA Immunization and Isolation of Functional Single-Domain Antibody Binders
Frederic Trempe, Martin A. Rossotti, Tahir Maqbool, C. Roger MacKenzie, and Mehdi Arbabi-Ghahroudi
4. Preparation of Immune and Synthetic VNAR Libraries as Sources of High-Affinity Binders
Jahaziel Gasperin-Bulbarela, Olivia Cabanillas-Bernal, Salvador Duenas, and Alexei F. Licea-Navarro
5. Isolation of Single-Domain Antibodies to Transmembrane Proteins Using Magnetized Yeast Cell Targets
Kaitlyn Bacon, Stefano Menegatti, and Balaji M. Rao
6. A Transgenic Heavy Chain IgG Mouse Platform as a Source of High Affinity Fully Human Single-Domain Antibodies for Therapeutic Applications
Dubravka Drabek, Rick Janssens, Rien van Haperen, and Frank Grosveld
Part III: Expression of Single-Domain Antibodies
7. Cytoplasmic Production of Nanobodies and Nanobody-Based Reagents by Co-Expression of Sulfhydryl Oxidase and DsbC Isomerase
Ario de Marco
8. Small Scale Secretory VHH Expression in Saccharomyces cerevisiae
Michiel M. Harmsen, Marga van Hagen-van Setten, and Peter T.J. Willemsen
9. Production of Single-Domain Antibodies in Pichia pastoris
Yusei Matsuzaki, Kaho Kajiwara, Wataru Aoki, and Mitsuyoshi Ueda
10. Production of Designer VHH-Based Antibodies in Plants
Henri De Greve
Part IV: Single-Domain Antibody Engineering and Characterization
11. Assessing the Aggregation Propensity of Single-Domain Antibodies Upon Heat-Denaturation Employing the Tm Shift
Patrick Kunz
12. Facile Affinity Maturation of Single-Domain Antibodies Using Next-Generation DNA Sequencing
Michael J. Lowden, Henk van Faassen, Shalini Raphael, Shannon Ryan, Greg Hussack, and Kevin A. Henry
13. Engineering pH-Sensitive Single-Domain Antibodies
Tosha M. Laughlin and James R. Horn
14. Humanization of Camelid Single-Domain Antibodies
Traian Sulea
15. Creation of Multimeric Single-Domain Antibodies Using Bacterial Superglues
Paul J. Wichgers Schreur, Sandra van de Water, and Jeroen Kortekaas
16. Introducing Cysteines into Nanobodies for Site-Specific Labelling
Simon Boje Hansen and Kasper Rojkjaer Andersen
17. Affinity-Guided Site Selective Labelling of Nanobodies With Aldehyde Handles
Anders Marcher and Kurt V. Gothelf
18. Cytoplasmic Expression of Nanobodies with Formylglycine Generating Enzyme Tag and Conversion to a Bio-Orthogonal Aldehyde Group
Da Li, Qiang Peng, Chungdong Huang, Berlin Zang, Jun Ren, Fangling Ji, Serge Muyldermans, and Lingyun Jia
19. Site-Specific Fluorescent Labelling, Single-Step Immunocytochemistry, and Delivery of Nanobodies into Living Cells
Jan Gettemans
20. Design and Validation of Site-Specifically Labelled Single-Domain Antibody-Based Tracers for In Vivo Fluorescence Imaging and Image-Guided Surgery
Noemi B. Declerck, Lukasz Mateusiak, and Sophie Hernot
21. Design and Preparation of Photobodies: Light-Activated Single-Domain Antibody Fragments
Zahide Yilmaz, Benedikt Jedlitzke, and Henning D. Mootz
Part V: Applications of Single-Domain Antibodies
22. Visualizing Filoviral Nucleoproteins Using Nanobodies Fused to the Ascorbate Peroxidase Derivatives APEX2 and dEAPX
Laura Jo Sherwood and Andrew Hayhurst
23. Development of Glypican-2 Targeting Single-Domain Antibody CAR T Cells for Neuroblastoma
Nan Li and Mitchell Ho
24. Single-Domain Antibodies for Intracellular Toxin Neutralization
Timothy F. Czajka and Nicholas J. Mantis
25. Generation of Single-Domain Antibody-Based Recombinant Immunotoxins
Bryan D. Fleming and Mitchell Ho
26. X-Ray Crystal Structure Analysis of VHH-Protein Antigen Complexes
Angham M. Ahmed and Cory L. Brooks
27. Functionalization of Magnetic Beads with Biotinylated Nanobodies for MALDI-TOF/MS-Based Quantitation of Small Analytes
Gabriel Lassabe, Macarena Pirez-Schirmer, and Gualberto Gonzalez-Sapienza
28. Nanobody-Based Assays for the Detection of Environmental and Agricultural Contaminants
Feng Wang and Hong Wang
29. Peptide Tag-Specific Nanobodies for Studying Proteins in Live Cells
Funmilayo O. Fagbadebo and Ulrich Rothbauer
30. Nanobody-Based GFP Traps to Study Protein Localization and Function in Developmental Biology
Shinya Matsuda, Gustavo Aguilar, M. Alessandra Vigano, and Markus Affolter
31. Optogenetic Activation of Intracellular Nanobodies
Daseuli Yu and Won Do Heo
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