Pericytes : methods and protocols
Author(s)
Bibliographic Information
Pericytes : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 2235）（Springer protocols）
Humana Press, c2021
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Includes bibliographical references and index
Description and Table of Contents
Description
This volume explores the techniques used to study human and animal pericytes. Chapters in this volume cover a range of topics such as pericyte dissociation from tissues, and analysis by immunohistochemistry and flow cytometry; avian chimaeras to investigate the embryonic development of cell lineage; pericyte purification by fluorescence activated cell sorting and ensuing culture; tissue turnover and regeneration; and the dynamic study of pericyte involvement in cancer dissemination, in situ, and in culture. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Pericytes: Methods and Protocols is a valuable resource that helps researchers define the biologic identity, functions, and potentials of a cell lineage of increasing interest and therapeutic significance.
Table of Contents
Preface...
Table of Contents...
Contributing Authors...
1. Visualizing Pericyte Mimicry of Angiotropic Melanoma by Direct Labeling of the Angioarchitecture
Roshini Prakash, Nikita Shivani Thareja, Thomas S. Carmichael, Raymond L. Barnhill, Claire Lugassy, and Laurent A. Bentolila
2. Transplantation of Purified Human Pericytes for Myocardial Repair in SCID-hu Mice
James E. Baily and William C.W. Chen
3. Assessment of Pericyte Phenotype by Flow Cytometry
N. S. Khan, C.C. West, F. Rossi, and M. Crisan
4. Characterization of Pericyte Phenotype In Vivo by Immunohistochemistry
C.C. West, N.S. Khan, and M. Crisan
5. A Metabolomics Based Approach to Identify Lineage Guiding Molecules in Pericyte Cultures
E.V. Alakpa, C.C. West, L. Goldie, M. Harper, K. Burgess, R. Ulijn, and M.J. Dalby
6. Pericyte Ontogeny: The Use of Chimeras to Track a Cell Lineage of Diverse Germ Line Origins
Heather C. Etchevers
7. Imaging Pericytes and the Regulation of Cerebral Blood Flow
Katie Boyd, Matthew Hammond-Haley, Rozan Vroman, and Catherine Hall
8. Derivation of Pericytes from Human Pluripotent Stem Cells
Ayelet Dar and Joseph Itskovitz-Eldor
9. Assessing the Bone-Forming Potential of Pericytes
Carolyn A. Meyers, Chenchao Wang, Jiajia Xu, Hsin Chuan Pan, Jia Shen, Kang Ting, Chia Soo, Bruno Peault, and Aaron W. James
10. Manipulating Pericyte Function with MicroRNAs
Milena Vitiello, Ben Cathcart, Andrea Caporali, and Marco Meloni
11. Isolation of Mesoangioblasts: A Subset of Pericytes with Strong Myogenic Potential from Mammals
Giorgia Giacomazzi, Gaia Giovannelli, Alessio Rotini, Domiziana Costamagna, Mattia Quattrocelli, and Maurilio Sampaolesi
12. Role of Pericytes in the Development of the Renin/Angiotensin System: Induction of Functional Renin in Cultures of Pericytes
Ania Stefanska, Angela Briski, Nusrat Khan, Christopher Kenyon, John J. Mullins, and Bruno Peault
13. Metastatic Dissemination Mimicked in a Multicellular Transwell Assay
Courtney Koenig and Anja Runge
Subject Index List...
by "Nielsen BookData"