Pseudogenes : functions and protocols

著者

• Poliseno, Laura

書誌事項

Pseudogenes : functions and protocols

edited by Laura Poliseno

（Methods in molecular biology / John M. Walker, series editor, 2324）（Springer protocols）

Humana Press, c2021

2nd ed

注記

Previous ed.: 2014

Includes bibliographical references and index

内容説明・目次

内容説明

This second edition explores techniques involving pseudogenic DNA, RNA, and proteins. Chapters describe how to identify pseudogenes and how to study the functions and alterations of pseudogenic RNA and proteins, both in vitro, and in vivo. Written in the highly successful Methods in Molecular Biology series format, methodological chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting while avoiding known pitfalls. Authoritative and cutting-edge, Pseudogenes: Functions and Protocols, Second Edition aims to be a practical guide to researches, useful to help further their study in this field.

目次

Part I: Introductory Chapter 1. Pseudogenes: Four Decades of Discovery Leonardo Salmena Part II: Methods for Pseudogene Identification 2. Methods to Identify and Study the Evolution of Pseudogenes using a Phylogenetic Approach Jacques Dainat and Pierre Pontarotti 3. Computational Methods For Pseudogene Annotation Based on Sequence Homology Paul M. Harrison 4. A Methodology to Study pseudogenized lincRNAs Sweta Talyan, Miguel A. Andrade-Navarro, and Enrique M. Muro Part III: Pseudogene Repositories 5. GENCODE Pseudogenes Cristina Sisu Part IV: Methods to Study Pseudogene-Related Alterations in Cancer 6. Comprehensive Detection of Pseudogenes Transcribed by Readthrough Rui Pinto, Daniel Sobral, and Ana Rita Grosso Part V: Methods to Study Transcribed Pseudogenes 7. Mathematical modeling of ceRNA-based interactions Andrew Dhawan 8. Pseudogenes as Competitive Endogenous RNAs: Target Prediction and Validation Florian A. Karreth, Ugo Ala, Paolo Provero, and Pier Paolo Pandolfi 9-Pseudogenes as Competitive Endogenous RNAs: Testing miRNA Dependency Xiaonan Xu and Florian A. Karreth 10. An Overview of the Computational Models Dealing with the Regulatory ceRNA Mechanism and ceRNA Deregulation in Cancer Federica Conte, Giulia Fiscon, Pasquale Sibilio, Valerio Licursi, and Paola Paci 11. Studying the Oncosuppressive Functions of PTENP1 as a ceRNA Glena Travis, Nahal Haddadi, Ann M. Simpson, Deborah J. Marsh, Eileen M. McGowan, and Najah T. Nassif 12. Methods to Study Protein-Binding to Pseudogene Transcripts Eusebio Chiefari, Biagio Arcidiacono, Maria Mirabelli, Francesco Saverio Brunetti, Domenica Maria Corigliano, Daniela Patrizia Foti, and Antonio Brunetti 13. Chromatin Regulation at Parental Gene Promoters by Pseudogene Sense lncRNAs Stefan Schoeftner, Michele Scarola, and Roberta Benetti 14.Pseudogenes: A Novel Source of Trans Acting Antisense RNAs Nicholas C. Lister, Per Johnsson, Paul D. Waters, and Kevin V. Morris Part VI: Methods to Study Translated Pseudogenes 15. Methods to Study Translated Pseudogenes: Recombinant Expression and Complementation, Targeted Proteomics and RNA Profiling Anne Parle-McDermott, Niamh Bookey, Paula Meleady and Paola Drago 16. A Restriction Endonuclease-Based Assay to distinguish NANOGP8 Retrogene from Parental NANOG Hamida Thakur and Abid R. Mattoo Part VII: CRISPR/CAS9 Technology Applied To Pseudogenes 17-CRISPR/Cas Technologies Applied to Pseudogenes Marianna Vitiello and Laura Poliseno Part VIII: In Vivo Models to Study Pseudogenes 18. Strategies to Study the Functions of Pseudogenes in Mouse Models of Cancer Ilah Bok and Florian A. Karreth Part IX: Clinical Relevance of Pseudogenes 19. Pseudogene Profiling For Cancer Subtype Classification Yan Zhang and Deyou Zheng 20. Pseudogenes as Biomarkers and Therapeutic Targets in Human Cancers Cristina Sisu 21. Methods for the Detection of Circulating Pseudogenes and their use as Cancer Biomarkers Manjusha Vaidya and Kiminobu Sugaya Part X: Methods To Avoid Pseudogene Detection 22. Dealing with Pseudogenes in Molecular Diagnostics in the Next Generation Sequencing Era Kathleen B.M. Claes, Toon Rosseel, and Kim De Leeneer

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詳細情報

• NII書誌ID(NCID): BC11639976
• ISBN:
  • 9781071615027
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: New York
• ページ数/冊数: xiii, 384 p.
• 大きさ: 27 cm
• 件名:
  • FREE : Molecular genetics
  • FREE : Genes
  • FREE : Human genetics
• 親書誌ID:
  • BA10794673
  • BA88873632