Optical tweezers : methods and protocols
著者
書誌事項
Optical tweezers : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 2478）（Springer protocols）
Humana Press/Springer, c2022
2nd ed
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
This detailed volume explores a wide variety of techniques involving optical tweezers, a technology that has become increasingly more accessible to a broad range of researchers. Beginning with recent technical advances, the book continues by covering the application of optical tweezers to study DNA-protein interactions and DNA motors, protocols to perform protein (un)folding experiments, the application of optical tweezers to study actin- and microtubule-associated motor proteins, and well as protocols for investigating the function and mechanical properties of microtubules and intermediate filaments, and more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Optical Tweezers: Methods and Protocols, Second Edition serves as an ideal resource for expanding the accessibility and use of optical traps by scientists of diverse disciplines.
目次
Part I: Historic Views on the Invention of Lasers and Optical Tweezers
1. The Invention of the Laser
Nick Taylor
2. Art Ashkin and the Origins of Optical Trapping and Particle Manipulation
Matthias D. Koch and Joshua W. Shaevitz
Part II: Technical Advances
3. Synthesis of Germanium Nanospheres as High Precision Optical Tweezers Probes
Swathi Sudhakar, Viktoria Wedler, Pasupathi Rajendran, and Erik Schaffer
4. Angular Optical Trapping to Directly Measure DNA Torsional Mechanics
Xiang Gao, James T. Inman, and Michelle D. Wang
5. Implementation of 3D Multi-Color Fluorescence Microscopy in a Quadruple Trap Optical Tweezers System
Anna E.C. Meijering, Julia A.M. Bakx, Tianlong Man, Iddo Heller, Gijs J.L. Wuite, and Erwin J.G. Peterman
6. One-Dimensional STED Microscopy in Optical Tweezers
Tianlong Man, Joost J. Geldhof, Erwin J.G. Peterman, Gijs J.L. Wuite, and Iddo Heller
7. Temperature Quantification and Temperature Control in Optical Tweezers
Joost J. Geldhof, Agata M. Malinowska, Gijs J.L. Wuite, Erwin J.G. Peterman, and Iddo Heller
8. High-Resolution Optical Tweezers Combined with Multi-Color Single-Molecule Microscopy
Rajeev Yadav, Kasun B. Senanayake, and Matthew J. Comstock
Part III: DNA, DNA Motors and DNA-Protein Interactions
9. Generating Negatively Supercoiled DNA Using Dual-Trap Optical Tweezers
Graeme A. King, Dian Spakman, Erwin J.G. Peterman, and Gijs J.L Wuite
10. Force-Activated DNA Substrates for In Situ Generation of ssDNA and Designed ssDNA/dsDNA Structures in an Optical-Trapping Assay
Arnulf M.K. Taylor, Stephen R. Okoniewski, Lyle Uyetake, and Thomas T. Perkins
11. Probing the Interaction between Chromatin and Chromatin-Associated Complexes with Optical Tweezers
Rachel Leicher and Shixin Liu
12. Simultaneous Mechanical and Fluorescence Detection of Helicase-Catalyzed DNA Unwinding
Lulu Bi, Zhenheng Qin, Xi-Miao Hou, Mauro Modesti, and Bo Sun
13. CRISPR/Cas9 On- and Off-Target Activity Using Correlative Force and Fluorescence Single-Molecule Microscopy
Matthew D. Newton, Benjamin J. Taylor, Maria Emanuela Cuomo, and David S. Rueda
Part IV: Protein (Un)Folding
14. Co-Temporal Single-Molecule Force and Fluorescence Measurements to Determine the Mechanism of Ribosome Translocation
Varsha P. Desai, Filipp Frank, and Carlos J. Bustamante
15. Using Single-Molecule Optical Tweezers to Study the Conformational Cycle of the Hsp90 Molecular Chaperone
Katarzyna Tych and Matthias Rief
16. Tethering Complex Proteins and Protein Complexes for Optical Tweezers Experiments
Kevin Maciuba and Christian M. Kaiser
17. Single-Molecule Manipulation Study of Chaperoned SNARE Folding and Assembly with Optical Tweezers
Huaizhou Jin, Jinghua Ge, Jie Yang, and Yongli Zhang
18. Using Optical Tweezers to Monitor Allosteric Signals through Changes in Folding Energy Landscapes
Lihui Bai, Mira Malmosi, Lydia Good, and Rodrigo A. Maillard
Part V: Cytoskeletal Motors and Proteins
19. High-Speed Optical Traps Address Dynamics of Processive and Non-Processive Molecular Motors
Lucia Gardini, Michael S. Woody, Anatolii V. Kashchuk, Yale E. Goldman, E. Michael Ostap, and Marco Capitanio
20. Microtubule Dumbbells to Assess the Effect of Force Geometry on Single Kinesin Motors
Serapion Pyrpassopoulos, Henry Shuman, and E. Michael Ostap
21. Single-Molecule Studies on the Motion and Force Generation of the Kinesin-3 Motor KIF1A
Lu Rao and Arne Gennerich
22. Ultra-Fast Force-Clamp Spectroscopy of Microtubule-Binding Proteins
Suvranta K. Tripathy, Vladimir M. Demidov, Ivan V. Gonchar, Shaowen Wu, Fazly I. Ataullakhanov, and Ekaterina L. Grishchuk
Part VI: Function and Mechanical Properties of Filaments
23. Catching the Conformational Wave: Measuring the Working Strokes of Protofilaments as They Curl Outward from Disassembling Microtubule Tips
Lucas E. Murray, Haein Kim, Luke M. Rice, and Charles L. Asbury
24. Mechanics of Single Vimentin Intermediate Filaments under Load
Anna V. Schepers, Julia Kraxner, Charlotta Lorenz, and Sarah Koester
25. Quantifying the Interaction Strength between Biopolymers
Charlotta Lorenz, Anna V. Schepers, and Sarah Koester
Part VII: Mechanosensing of Membrane Channels
26. Measuring T Cell Receptor-Mediated Mechanosensing Using Optical Tweezers Combined with Fluorescence Imaging
Hannah M. Stephens, Kristine N. Brazin, Robert J. Mallis, Yinnian Feng, Debasis Banik, Ellis L. Reinherz, and Matthew J. Lang
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