Fusarium wilt : methods and protocols
Author(s)
Bibliographic Information
Fusarium wilt : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 2391）（Springer protocols）
Humana Press, c2022
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Note
Includes bibliographical references and index
Description and Table of Contents
Description
This volume provides a collection of molecular protocols detailing the most common and modern techniques on fusarium wilt. Chapters guide readers through methods on initial isolation, molecular-based identification, genome characterization, generation of mutants, and characterization of interactions with other organisms including host plants. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
Authoritative and cutting-edge, Fusarium wilt: Methods and Protocols aims to be a valuable resource for mycologists, plant pathologists, microbiologists, geneticists, and other scientists that have an interest in members of the Fusarium oxysporum species complex or closely related fungi.
Table of Contents
1. Pure culture and DNA sequence-based identification of Fusarium from symptomatic plants and diverse substrates
Kerry O'Donnell, Imana Laraba, and David M. Geiser
2. Isolation of high-molecular weight (HMW) DNA from Fusarium oxysporum for long-read sequencing
Zunyong Liu, Eun-Gyu No, Catherine Danmaigona Clement, Ping He, and Libo Shan
3. Karyotyping of Fusarium oxysporum by pulsed field gel electrophoresis and the germ tube burst method
Yu Ayukawa and Masatoki Taga
4. RNA-seq data analysis for differential expression
Navdeep Gill and Braham Dhillon
5. Quantification and isolation of spontaneous colony growth variants
Cristina Lopez Diaz, Lucia Gomez-Gil, Elena Perez-Nadales, Gesabel Navarro Velasco, and Antonio Di Pietro
6. Generation of a T-DNA insertion mutant library of Fusarium oxysporum using Agrobacterium tumefaciens
Yan-Hong Dong and Shu-Tong Wang
7. Targeted gene disruption via CRISPR/Cas9 ribonucleoprotein complexes in Fusarium oxysporum
Ambika Pokhrel, Seungyeon Seo, Qiang Wang, and Jeffrey J. Coleman
8. CRISPR/Cas9 RNP-mediated gene fusion to assess protein quantification and subcellular localization in Fusarium oxysporum
Qiang Wang and Jeffrey J. Coleman
9. In vivo monitoring of cytosolic pH using the ratiometric pH sensor pHluorin
Tania R. Fernandes, Antonio Serrano, and Antonio Di Pietro
10. Multi-pronged investigation of volatile compound-mediated interactions of Fusarium oxysporum with plants, fungi and bacteria
Ningxiao Li and Seogchan Kang
11. Isolation of mitochondria from Fusarium species
Michael MacKillop and John C. Kennell
12. In planta gene expression analysis and colonization of Fusarium oxysporum
Virginia Casado-del Castillo, Ernesto Perez Benito, and Jose Maria Diaz-Minguez
13. Time-lapse imaging of root pathogenesis and fungal proliferation without physically disrupting roots
Hye-Seon Kim, Sook-Young Park, Seogchan Kang, and Kirk Czymmek
14. High-throughput screening assays to identify plant natural products with antifungal properties against Fusarium oxysporum
Yong Zhang, Pei-Lun Kao, Akaansha Rampal, Sibongile Mafu, Sergey N. Savinov, and Li-Jun Ma
15. Screening and assessment of pisatin demethylase activity (PDA)
Catherine C. Wasmann and Jeffrey J. Coleman
16. Detection and genotyping of Fusarium oxysporum f. sp. vasinfectum race 4 (VCG0114), the Fusarium wilt pathogen of cotton
Jinggao Liu and Tanya A. Wagner
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