The blood-brain barrier : methods and protocols

著者

• Stone, Nicole

書誌事項

The blood-brain barrier : methods and protocols

edited by Nicole Stone

（Methods in molecular biology / John M. Walker, series editor, 2492）（Springer protocols）

Humana Press, c2022

注記

Includes bibliographical references and index

内容説明・目次

内容説明

This detailed volume features techniques to explore the complex interface that separates the systemic circulation from the central nervous system, known as the blood-brain barrier (BBB). Beginning with an introduction to its physiology, the book continues with sections on using pluripotent stem cells in models of the BBB, co-culture, permeability and transwell models, microfluidic and chip models, as well as models to study specific BBB pathologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, The Blood-Brain Barrier: Methods and Protocols collects a wide range of methodologies which will aid all researchers in the fascinating world of the blood-brain barrier.

目次

Part I: Introduction to Blood-Brain Barrier (BBB) Physiology 1. Cells of the Blood-Brain Barrier: An Overview of the Neurovascular Unit in Health and Disease Heather L. McConnell and Anusha Mishra 2. In Vitro Models of the Blood-Brain Barrier Snehal Raut, Aditya Bhalerao, Behnam Noorani, and Luca Cucullo Part II: Using Pluripotent Stem Cells in Models of the BBB 3. In Vitro Models of the Human Blood-Brain Barrier Based on Human Induced Pluripotent Stem Cells: Opportunities and Challenges Iqra Pervaiz and Abraham J. Al-Ahmad 4. Induced Pluripotent Stem Cell (iPSC)-Derived Endothelial Cells to Study Bacterial-Brain Endothelial Cell Interactions Eric R. Espinal, S. Jerod Sharp, and Brandon J. Kim 5. An hiPSC-Derived In Vitro Model of the Blood-Brain Barrier Mary Goodwin-Trotman, Krushangi Patel, and Alessandra Granata 6. A Three-Dimensional Brain-on-Chip Using Human iPSC-Derived GABAergic Neurons and Astrocytes Lumei Liu, Youngmi Koo, Teal Russell, and Yeoheung Yun Part III: Co-Culture, Permeability, and Transwell Models of the BBB: Methods for Studying Shear Stress, Barrier Integrity and Breakdown 7. An Improved In Vitro Porcine Blood-Brain Barrier Model for Permeability Screening and Functional Studies Adjanie Patabendige 8. Establishment of an In Vitro Model of Human Blood-Brain Barrier to Study the Impact of Ischaemic Injury Rais Reskiawan A. Kadir, Mansour Alwjwaj, and Ulvi Bayraktutan 9. A Novel Dynamic Human In Vitro Model for Studying the Blood-Brain Barrier Patricia Miranda-Azpiazu and Sikha Saha 10. A Dynamic, In Vitro BBB Model to Study the Effects of Varying Levels of Shear Stress Gemma Molins Gutierrez, Jordi Martorell, Antonio G. Salazar-Martin, and Mercedes Balcells Part IV: Microfluidic and Chip Models of the BBB 11. Novel, Emerging Chip Models of the BBB and Future Directions Paul M. Holloway 12. Integrating Primary Astrocytes in a Microfluidic Model of the Blood-Brain Barrier Eliana Lauranzano, Marco Rasile, and Michela Matteoli 13. Fabrication of Microtube-Embedded Chip to Mimic Blood-Brain Barrier Capillary Vessels Dilshan Sooriyaarachchi, Shahrima Maharubin, and George Z. Tan 14. BBB-on-a-Chip: Modeling Functional Human BBB by Mimicking 3D Brain Angiogenesis Using Microfluidic Chip Somin Lee, Minhwan Chung, and Noo Li Jeon Part V: Models to Study Specific Pathologies at the BBB: Cancer, Neurodegeneration, and More 15. Cell Interplay Model to Assess the Impact of Glioma Cells on Blood-Brain Barrier Permeability Claudia Martins and Bruno Sarmento 16. An In Vitro Human Blood-Brain Barrier Model to Study Breast Cancer Brain Metastasis Caroline Mysiorek, Lucie Dehouck, Fabien Gosselet, and Marie-Pierre Dehouck 17. An In Vivo Mouse Model to Study Blood-Brain Barrier Destabilization in the Chronic Phase of Stroke Svetlana M. Stamatovic, Chelsea M. Phillips, Richard F. Keep, and Anuska V. Andjelkovic 18. Immunohistochemical Analysis of Tight Junction Proteins Chris Greene and Matthew Campbell 19. An In Vitro BBB Model to Study Firm Shear-Stress Resistant Leukocyte Adhesion to Human Brain Endothelial Cells Camilla Cerutti and Ignacio A. Romero 20. An In Vitro Model of the Blood-Brain Barrier (BBB) to Study Alzheimer's Disease: The Role of -Amyloid and Its Influence on PBMC Infiltration Simona Federica Spampinato, Yukio Takeshita, and Birgit Obermeier

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詳細情報

• NII書誌ID(NCID): BC13249280
• ISBN:
  • 9781071622889
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: New York
• ページ数/冊数: xi, 357 p.
• 大きさ: 27 cm
• 分類:
  • DC23 : 612.824
• 件名:
  • LCSH : Blood-brain barrier -- Research -- Methodology
• 親書誌ID:
  • BA10794673
  • BA88873632