Antibody arrays : methods and protocols

著者

    • Whittaker, Kelly C.
    • Huang, Ruo-Pan

書誌事項

Antibody arrays : methods and protocols

edited by Kelly C. Whittaker and Ruo-Pan Huang

(Methods in molecular biology / John M. Walker, series editor, 2237)(Springer protocols)

Humana Press, c2021

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

This detailed book presents a technical overview and practical methodology of a variety of antibody array formats and technologies. As advantages and disadvantages of antibody array types are explored, the volume also delves into practical applications of antibody arrays pertaining to investigations of specific research topics and biological processes as well as guidance on the methods of processing, analysis, and storage of array data. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Antibody Arrays: Methods and Protocols aims to empower the reader with the information required to select the most appropriate array for their research application, with the technical knowledge to use and process the array, and with the knowledge to perform analysis that realizes the maximum benefit from the data generated.

目次

1. Sandwich-Based Antibody Arrays for Protein Detection Ying Qing Mao, Brianne Petritis, and Ruo-Pan Huang 2. Well-Based Antibody Arrays Robert S. Matson 3. One-Step Antibody Arrays Ying Qing Mao and Lin-hai Li 4. Global Detection of Proteins by Label-Based Antibody Array Zhizhou Kuang, Li-Pai Chen, Ruochun Huang, and Ruo-Pan Huang 5. Surface Plasmon Resonance Imaging-Mass Spectrometry Coupling on Antibody Array Biochip: Multiplex Monitoring of Biomolecular Interactions and On-Chip Identification of Captured Antigen Anastasiia Halushkina, William Buchmann, Nathalie Jarroux, and Regis Daniel 6. Multiplexed Protein Biomarker Detection with Microfluidic Electrochemical Immunoarrays Abby Jones, Patricia Czarnecki, Lasangi Dhanapala, and James F. Rusling 7. Cytometry Multiplex Bead Antibody Array Benyue Zhang, Gang Xiao, Ying Qing Mao, Zhiqiang Lv, and Ruo-Pan Huang 8. Antibody Arrays: Barcode Technology Liwei Yang and Jun Wang 9. Reverse Phase Protein Arrays Justin B. Davis, Sydney Andes, and Virginia Espina 10. Enhanced Protein Profiling Arrays for High-Throughput Quantitative Measurement of Cytokine Expression Ruochun Huang, Chunhui Gao, Wenjuan Wu, and Ruo-Pan Huang 11. Methods for Membranes and Other Absorbent Surfaces Jarad J. Wilson, Yun Xi, Ruochun Huang, and Ruo-Pan Huang 12. Precise Chip-to-Chip Reagent Transfer for Cross-Reactivity-Free Multiplex Sandwich Immunoassays Francois Paquet-Mercier, David Juncker, and Sebastien Bergeron 13. Antibody Printing Technologies Valentin Romanov and Benjamin D. Brooks 14. Self-Assembling Peptide Hydrogels for 3D Microarrays Greta Bergamaschi, Alessandro Strada, Roberto Frigerio, Marina Cretich, and Alessandro Gori 15. Surface Modification of Glass Slides with Aminosilanes for Microarray Use Chris Stuart 16. Dried Blood-Based Protein Profiling Using Antibody Arrays Kelly C. Whittaker, Ying Qing Mao, Siwei Zhu, Zhiqiang Lv, and Ruo-Pan Huang 17. Profiling Glycoproteins on Functionalized Reverse Phase Protein Array Ying Zhang, Liyuan Zhang, Anton Iliuk, and W. Andy Tao 18. Determining Protein Phosphorylation Status Using Antibody Arrays and Phos-Tag Biotin Eiji Kinoshita, Emiko Kinoshita-Kikuta, and Tohru Koike 19. Analyzing Signaling Pathways Using Antibody Arrays Hao Tang, Chaohui Duan, Zhizhou Kuang, and Ruo-Pan Huang 20. Using Antibody Arrays for Biomarker Discovery Shuhong Luo, Ling Yunbiao, Li-Pai Chen, and Ruo-Pan Huang 21. Immune Cell Isolation from Murine Intestine for Antibody Array Analysis Joshua A. Owens and Rheinallt M. Jones 22. Database Development for Antibody Arrays Zhaowei Xu, Likun Huang, and Sheng-ce Tao 23. Data Analysis for Antibody Arrays Huihua Zhang, Ying Qing Mao, Brianne Petritis, and Ruo-Pan Huang 24. Statistical Analysis Options for Antibody Array Data Jingqiao Lu

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詳細情報

  • NII書誌ID(NCID)
    BC13502757
  • ISBN
    • 9781071610633
  • 出版国コード
    us
  • タイトル言語コード
    eng
  • 本文言語コード
    eng
  • 出版地
    New York
  • ページ数/冊数
    xi, 316 p.
  • 大きさ
    27 cm
  • 親書誌ID
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