Antibody arrays : methods and protocols
著者
書誌事項
Antibody arrays : methods and protocols
(Methods in molecular biology / John M. Walker, series editor, 2237)(Springer protocols)
Humana Press, c2021
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
This detailed book presents a technical overview and practical methodology of a variety of antibody array formats and technologies. As advantages and disadvantages of antibody array types are explored, the volume also delves into practical applications of antibody arrays pertaining to investigations of specific research topics and biological processes as well as guidance on the methods of processing, analysis, and storage of array data. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Antibody Arrays: Methods and Protocols aims to empower the reader with the information required to select the most appropriate array for their research application, with the technical knowledge to use and process the array, and with the knowledge to perform analysis that realizes the maximum benefit from the data generated.
目次
1. Sandwich-Based Antibody Arrays for Protein Detection
Ying Qing Mao, Brianne Petritis, and Ruo-Pan Huang
2. Well-Based Antibody Arrays
Robert S. Matson
3. One-Step Antibody Arrays
Ying Qing Mao and Lin-hai Li
4. Global Detection of Proteins by Label-Based Antibody Array
Zhizhou Kuang, Li-Pai Chen, Ruochun Huang, and Ruo-Pan Huang
5. Surface Plasmon Resonance Imaging-Mass Spectrometry Coupling on Antibody Array Biochip: Multiplex Monitoring of Biomolecular Interactions and On-Chip Identification of Captured Antigen
Anastasiia Halushkina, William Buchmann, Nathalie Jarroux, and Regis Daniel
6. Multiplexed Protein Biomarker Detection with Microfluidic Electrochemical Immunoarrays
Abby Jones, Patricia Czarnecki, Lasangi Dhanapala, and James F. Rusling
7. Cytometry Multiplex Bead Antibody Array
Benyue Zhang, Gang Xiao, Ying Qing Mao, Zhiqiang Lv, and Ruo-Pan Huang
8. Antibody Arrays: Barcode Technology
Liwei Yang and Jun Wang
9. Reverse Phase Protein Arrays
Justin B. Davis, Sydney Andes, and Virginia Espina
10. Enhanced Protein Profiling Arrays for High-Throughput Quantitative Measurement of Cytokine Expression
Ruochun Huang, Chunhui Gao, Wenjuan Wu, and Ruo-Pan Huang
11. Methods for Membranes and Other Absorbent Surfaces
Jarad J. Wilson, Yun Xi, Ruochun Huang, and Ruo-Pan Huang
12. Precise Chip-to-Chip Reagent Transfer for Cross-Reactivity-Free Multiplex Sandwich Immunoassays
Francois Paquet-Mercier, David Juncker, and Sebastien Bergeron
13. Antibody Printing Technologies
Valentin Romanov and Benjamin D. Brooks
14. Self-Assembling Peptide Hydrogels for 3D Microarrays
Greta Bergamaschi, Alessandro Strada, Roberto Frigerio, Marina Cretich, and Alessandro Gori
15. Surface Modification of Glass Slides with Aminosilanes for Microarray Use
Chris Stuart
16. Dried Blood-Based Protein Profiling Using Antibody Arrays
Kelly C. Whittaker, Ying Qing Mao, Siwei Zhu, Zhiqiang Lv, and Ruo-Pan Huang
17. Profiling Glycoproteins on Functionalized Reverse Phase Protein Array
Ying Zhang, Liyuan Zhang, Anton Iliuk, and W. Andy Tao
18. Determining Protein Phosphorylation Status Using Antibody Arrays and Phos-Tag Biotin
Eiji Kinoshita, Emiko Kinoshita-Kikuta, and Tohru Koike
19. Analyzing Signaling Pathways Using Antibody Arrays
Hao Tang, Chaohui Duan, Zhizhou Kuang, and Ruo-Pan Huang
20. Using Antibody Arrays for Biomarker Discovery
Shuhong Luo, Ling Yunbiao, Li-Pai Chen, and Ruo-Pan Huang
21. Immune Cell Isolation from Murine Intestine for Antibody Array Analysis
Joshua A. Owens and Rheinallt M. Jones
22. Database Development for Antibody Arrays
Zhaowei Xu, Likun Huang, and Sheng-ce Tao
23. Data Analysis for Antibody Arrays
Huihua Zhang, Ying Qing Mao, Brianne Petritis, and Ruo-Pan Huang
24. Statistical Analysis Options for Antibody Array Data
Jingqiao Lu
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