Developmental biology of the sea urchin and other marine invertebrates : methods and protocols
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Bibliographic Information
Developmental biology of the sea urchin and other marine invertebrates : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 2219）（Springer protocols）
Humana Press, c2021
2nd ed.
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Note
Includes bibliographical references and index
Description and Table of Contents
Description
This detailed second edition presents a wide variety of marine invertebrate model systems, from cephalochordata to holothurians, along with novel experimental protocols for taking advantage of their unique properties. The techniques range from culturing the organisms to modifying their DNA. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Developmental Biology of the Sea Urchin and Other Marine Invertebrates: Methods and Protocols, Second Edition is an ideal guide for researchers working with these versatile organisms and for furthering our understanding of fundamental biological questions.
Table of Contents
1. Laboratory Culture and Mutagenesis of Amphioxus (Branchiostoma floridae)
Linda Z. Holland and Guang Li
2. Handling and Manipulation of Gametes and Embryos of the Annelidan Worm Pseudopotamilla occelata
Ryusaku Deguchi and Takeshi Nakano
3. The Starfish Asterina pectinifera: Collection and Maintenance of Adults and Rearing and Metamorphosis of Larvae
Naoyuki Murabe, Ei-ichi Okumura, Kazuyoshi Chiba, Enako Hosoda, Susumu Ikegami, and Takeo Kishimoto
4. Experimental Tools to Study Regeneration in the Sea Anemone Nematostella vectensis
Aldine R. Amiel and Eric Roettinger
5. Staining and Tracking Methods for Studying Sponge Cell Dynamics
Carole Borchiellini, Sandie M. Degnan, Emilie Le Goff, Caroline Rocher, Amelie Vernale, Stephen Baghdiguian, Nina Sejourne, Florent Marschal, Andre Le Bivic, Nelly Godefroy, Bernard M. Degnan, and Emmanuelle Renard
6. Microscopy Studies of Placozoans
Carolyn L. Smith, Tatiana D. Mayorova, Christine A. Winters, Thomas S. Reese, Sally P. Leys, and Andreas Heyland
7. Identification of SH2 Domain-Mediated Protein Interactions that Operate at Fertilization in the Sea Star Patiria miniata
Lauren Bates, Emily Wiseman, Jamie Kitson, and David J. Carroll
8. Marine Nemertean Worms for Immunoblotting Studies of Oocyte Aging
Stephen A. Stricker
9. Recovery of Sea Star Egg Cell Surface Proteins Released at Fertilization
Emily Wiseman, Lauren Bates, and David J. Carroll
10. Quantifying Cell Proliferation During Regeneration of Aquatic Worms
Eduardo E. Zattara and B. Duygu OEzpolat
11. In Situ Hybridization Techniques in the Homoscleromorph Sponge Oscarella lobularis
Laura Fierro-Constain, Caroline Rocher, Florent Marschal, Quentin Schenkelaars, Nina Sejourne, Carole Borchiellini, and Emmanuelle Renard
12. Methodology for Whole Mount and Fluorescent RNA In Situ Hybridization in Echinoderms: Single, Double, and Beyond
Margherita Perillo, Periklis Paganos, Maxwell Spurrell, Maria I. Arnone, and Gary M. Wessel
13. Gene Editing in the Ascidian Phallusia mammillata and Tail Nerve Cord Formation
Alex McDougall, Celine Hebras, Isa Gomes, and Remi Dumollard
14. Transcriptomic Analysis in the Sea Anemone Nematostella vectensis
Jacob F. Warner and Eric Roettinger
15. RNA Interference on Regenerating Holothurian Gut Tissues
Miosotis Alicea-Delgado, Samir A. Bello-Melo, and Jose E. Garcia-Arraras
16. ATAC-Seq for Assaying Chromatin Accessibility Protocol Using Echinoderm Embryos
Marta Silvia Magri, Danila Voronov, Jovana Randelovic, Claudia Cuomo, Jose Luis Gomez-Skarmeta, and Maria I. Arnone
17. Usage of the Sea Urchin Hemicentrotus pulcherrimus Database, HpBase
Sonoko Kinjo, Masato Kiyomoto, Takashi Yamamoto, Kazuho Ikeo, and Shunsuke Yaguchi
18. Functional Genomic Studies of Trichoplax adhaerens Neuronal Activity
Julia Gauberg, Adriano Senatore, and Andreas Heyland
19. A Bioinformatics Tutorial for Comparative Development Genomics in Diverse Meiofauna
Joseph L. Sevigny, Jon L. Norenburg, and Francesca Leasi
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