Protein nanotechnology : protocols, instrumentation, and applications

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書誌事項

Protein nanotechnology : protocols, instrumentation, and applications

edited by Juliet A. Gerrard, Laura J. Domigan

(Methods in molecular biology / John M. Walker, series editor, 2073)(Springer protocols)

Humana Press, c2020

3rd ed

  • : pbk.

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

This third edition volume expands on the previous editions with updated approaches and techniques used to study protein nanotechnology and the future of nanomaterial compositions. This book is organized into Three Parts: Part One looks at recombinant protein expression in insect cells, and methods to produce molecular motors, molecular superglues, and protein templates; Part Two explores functionalization strategies and ways to incorporate functional protein components into nanodevices; Part Three discusses various instrumental techniques used to study protein nanostructures. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Protein Nanotechnology: Protocols, Instrumentation, and Applications, Third Edition is a valuable resource for any researchers looking to expand their knowledge in this evolving field.

目次

Preface... Table of Contents... Contributing Authors... 1. Introduction to Protein Nanotechnology Laura J. Domigan and Juliet A. Gerrard Part I Design and Production of Protein Building Blocks for Nanotechnology 2. Protein Expression in the Baculovirus-Insect Cell Expression System Adetayo A. Adeniyi and Linda Hwee-Lin Lua 3. Peroxiredoxin Proteins as Building Blocks for Nanotechnology Frankie Conroy and N. Amy Yewdall 4. Formation of Amphipathic Monolayers from Fungal Hydrophobin Proteins Sarah R. Ball, Chi L. L. Pham, Victor Lo, Ann H. Kwan, and Margaret Sunde 5. ATP Synthase: Expression, Purification, and Function Meghna Sobti, Robert Ishmukhametov, and Alastair G. Stewart 6. Molecular Superglues: Discovery and Engineering Orthogonalization Paul Young and Christopher Squires 7. Production of Multicomponent Protein Templates for the Positioning and Stabilization of Enzymes Samuel Lim, Douglas S. Clark, and Dominic J. Glover Part II Towards Protein Nano-Devices 8. Adding Function to Protein Scaffolds Kyle Webster, Luigi Sasso, and Laura J. Domigan 9. Virus-Derived Nanoparticles Noor H. Dashti and Frank Sainsbury 10. Strategies for Increasing Protein Stability Peter G. Chandler, Sebastian S. Broendum, Blake T. Riley, Matthew A. Spence, Colin J. Jackson, Sheena McGowan, and Ashley M. Buckle 11. Generation of High Affinity Molecularly Imprinted Nanoparticles for Protein Recognition via a Solid-Phase Synthesis Protocol Francesco Canfarotta, Sergey A. Piletsky, and Nicholas W. Turner 12. Nanotechnology with S-Layer Proteins Bernhard Schuster and Uwe B. Sleytr Part III Meaningful Measurements of Protein Nanocomponents 13. Preparation of Proteins and Macromolecular Assemblies for Cryo-Electron Microscopy Lou Brillault and Michael J. Landsberg 14. Atomic Force Microscopy of Proteins Yiran An, Sesha Sarathchandra Manuguri, and Jenny Malmstrom 15. Native Protein Mass Spectrometry Timothy M. Allison and Mark T. Agasid 16. Nanoparticle Tracking Analysis of b-Casein Nanocarriers Hikmat Masri and Jared K. Raynes 17. Molecular Dynamics Simulation of Proteins Thomas A. Collier, Thomas J. Piggot, and Jane R. Allison

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