Stem cell renewal and cell-cell communication : methods and protocols

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書誌事項

Stem cell renewal and cell-cell communication : methods and protocols

edited by Kursad Turksen

（Methods in molecular biology / John M. Walker, series editor, 2346）（Springer protocols）

Humana Press, c2021

2nd ed

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

This detailed book brings together a new set of protocols to arm cell biologists with techniques that are currently being used in a number of well-established laboratories around the world. The contents represent the great strides made in the field of cell-cell communications with respect to the identification and characterization of key components of the communication apparatus, assembly and maintenance of the communications structures, and concomitantly their roles in not only tissue formation and maintenance but also regeneration and repair. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective chapters, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Stem Cell Renewal and Cell-Cell Communication: Methods and Protocols, Second Edition serves as an ideal guide for experts and newcomers alike seeking to increase our understanding of the crucial biological and physiological roles of cell-cell communications in tissue function and organismal integrity.

目次

1. Inference of Ligand-Receptor Pairs from Single-Cell Transcriptomics Data Mirjana Efremova and Roser Vento-Tormo 2. Multiple Imaging Modalities for Cell-Cell Communication via Calcium Mobilizations in Corneal Epithelial Cells Yoonjoo K. Lee, Kristen L. Segars, and Vickery Trinkaus-Randall 3. Interactions of Hematopoietic Stem Cells with Bone Marrow Niche Xinghui Zhao, Cuiping Zhang, Xiaojing Cui, and Ying Liang 4. Ex Vivo Modeling of Hematopoietic Stem Cell Homing to the Fetal Liver Amina Mohammadalipour, Miguel F. Diaz, Sumedha Pareek, and Pamela L. Wenzel 5. Analysis of Epithelial Architecture and Planar Spindle Orientation in the Drosophila Wing Disc Yu-ichiro Nakajima 6. A Co-Culture Model to Study the Effect of Kidney Fibroblast-p90RSK on Epithelial Cell Survival Ling Lin, Samantha White, and Kebin Hu 7. Calcium Fluorescence Recordings from Neuroepithelial Stem Cells Masayuki Yamashita 8. Ultrastructural Analysis of Cell-Cell Interactions in Drosophila Ovary Matthew Antel, Valentina Baena, Mark Terasaki, and Mayu Inaba 9. TIRF Microscopy as a Tool to Determine Exosome Composition Noa B. Martin-Cofreces, Daniel Torralba, Marta Lozano-Prieto, Nieves Fernandez-Gallego, and Francisco Sanchez-Madrid 10. Rapid Visualization of Intracellular Vesicle Events during Synaptic Stimulation Noa B. Martin-Cofreces, Amelia Rojas-Gomez, Sara G. Dosil, Irene Fernandez-Delgado, and Francisco Sanchez-Madrid 11. Monitoring of Active Notch Signaling in Mouse Bladder Urothelium Panagiotis Karakaidos and Theodoros Rampias 12. Examining Local Cell-to-Cell Signaling in the Kidney Using ATP Biosensing Gareth W. Price, Joe A. Potter, Bethany M. Williams, Chelsy L. Cliff, Mark J. Wall, Claire E. Hills, and Paul E. Squires 13. Isolation and Assessment of Pancreatic Islets Versus Dispersed Beta Cells: A Straightforward Approach to Examine Cell-Cell Communication Rachel T. Scarl, William J. Koch, Kathryn L. Corbin, and Craig S. Nunemaker 14. Promoter Pull-Down Assay: A Biochemical Screen for DNA-Binding Proteins Ryan R. Chaparian and Julia C. van Kessel 15. Purification of the Vibrio Quorum-Sensing Transcription Factors LuxR, HapR, and SmcR Jane D. Newman and Julia C. van Kessel 16. Preserving Cytonemes for Immunocytochemistry of Cultured Adherent Cells Sally Rogers and Steffen Scholpp 17. Fluorescent Labeling of Connexin with As-Complex and X-Y Coordinates Registration of Target Single-Cells Based on a Triangle Standard Chip for the Image Analysis of Gap Junctional Communication Mikako Saito 18. Chemical and Voltage Gating of Gap Junction Channels Expressed in Xenopus Oocytes Camillo Peracchia 19. The Analysis of Gap Junctional Intercellular Communication among Osteocytes in Chick Calvariae by Fluorescence Recovery after Photobleaching Ziyi Wang, Yoshihito Ishihara, and Hiroshi Kamioka 20. Flow Cytometry Evaluation of Gap Junction-Mediated Intercellular Communication between Cytotoxic T Cells and Target Tumor Cells Mariela Navarrete, Flavio Salazar-Onfray, and Andres Tittarelli

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