Neutrophil : methods and protocols

著者

• Quinn, Mark T.
• Deleo, Frank R.

書誌事項

Neutrophil : methods and protocols

edited by Mark T. Quinn, Frank R. DeLeo

（Methods in molecular biology / John M. Walker, series editor, 2087）（Springer protocols）

Humana Press, c2020

3rd ed

• : [pbk]

注記

Includes bibliographical references and index

内容説明・目次

内容説明

Neutrophils, the most abundant white cells in humans, serve as the primary cellular defense against infection. This volume provides a concise set of protocols for assessing basic neutrophil functions and investigating specialized areas in neutrophil biology. Each of the protocols is written by leading researchers in the field and includes hints for success, as well as guidance for troubleshooting. In the third edition of Neutrophils: Methods and Protocols, chapters retained from previous editions have been have been updated and include many new approaches. In addition, the Third Edition contains a number of new chapters that were not included in the first or second editions. Part I contains overviews of neutrophil biology, function, and disorders. Part II describes commonly used methods to isolate neutrophils from humans and other animal species. Part III details methods for investigating chemotaxis, transmigration, phagocytosis, and bactericidal activity. Part IV includes protocols that measure neutrophil apoptosis, calcium signal transduction, degranulation and detection of cytoplasmic granules, gene expression, transcription factors, and apoptosis. Part V provides multiple assays for measuring production of intracellular and/or extracellular reactive oxygen species, and a method that details use of the cell-free NADPH oxidase assay, an iconic assay for studies of the neutrophil NADPH oxidase. Part VI provides chapters that describe how to analyze formation and function of neutrophil extracellular traps. Neutrophil Methods and Protocols: Third Edition is a comprehensive source for detailed explanations and applications of the most modern methodological advances in neutrophil biology. Both basic scientists and clinicians will find a collection of this caliber to be an invaluable aid in their work with neutrophils. Features * Provides neutrophil protocols that are broadly applicable to leukocyte biology * Includes protocols for isolation of neutrophils from humans and animal species * Presents an interesting and informative overview of the field of neutrophil biology * Facilitates a better understanding of the role of neutrophils in host defense Readership Molecular and Cell Biologists, Immunologists, Infectious Disease Specialists, Microbiologists

目次

Dedication... Preface... Table of Contents... Contributing Authors... Part I: Neutrophils and Neutrophil Disorders: Overviews 1. The Role of Neutrophils in the Immune System: An Overview Harry L. Malech, Frank R. DeLeo, and Mark T. Quinn 2. Neutrophil Defects and Diagnosis Disorders of Neutrophil Function: An Overview Mary C. Dinauer Part II: Neutrophil Isolation 3. Isolation of Human Neutrophils from Venous Blood Silvie Kremserova and William M. Nauseef 4. Isolation of Neutrophils from Non-Human Species Daniel W. Siemsen, Lilya N. Kirpotina, Natalia Malachowa, Igor A. Schepetkin, Adeline R. Porter, Benfang Lei, Frank R. DeLeo, and Mark T. Quinn 5. Isolation of Neutrophils from Larval Zebrafish and their Transplantation into Recipient Larvae for Functional Studies Hannah Darroch, Jonathan W. Astin, and Christopher J. Hall Part III: Neutrophil Chemotaxis, Phagocytosis, and Bactericidal Activity 6. Analysis of Neutrophil Transmigration through Epithelial Cell Monolayers Liliya N. Kirpotina, Douglas J. Kominsky, Mark T. Quinn, and Steve D. Swain 7. Quantification of Chemotaxis or Respiratory Burst using Ex Vivo Culture-Derived Murine Neutrophils Klaudia Szymczak, Margery G.H. Pelletier, and Peter Gaines 8. Ex Vivo Human Neutrophil Swarming against Live Microbial Targets Alex Hopke and Daniel Irimia 9. Micro-Injection and Micropipette Controlled Phagocytosis Methods for Neutrophils Maurice B. Hallett, Jennie S. Campbell, Iraj Laffafian, and Sharon Dewitt 10. Using Imaging Flow Cytometry to Quantify Neutrophil Phagocytosis Asya Smirnov, Michael D. Solga, Joanne Lannigan, and Alison K. Criss 11. Visualization and Quantification of Phagocytosis by Neutrophils Gaelen Guzman and Fikadu G. Tafesse 12. Analysis of Neutrophil Bactericidal Activity Nicholas J. Magon, Heather A. Parker, Louisa V. Ashby, Reuben J. Springer, and Mark B. Hampton Part IV: Biochemistry, Biology, and Signal Transduction of Neutrophils 13. Assessment of Neutrophil Apoptosis Nicole D. Barth, Marc Vendrell, David A. Dorward, Adriano G. Rossi, and Ian Dransfield 14. Optical Methods for the Measurement and Manipulation of Cytosolic Calcium Signals in Neutrophils Maurice B. Hallett, Rhiannon E. Roberts, and Sharon Dewitt 15. Labeling Acidic Compartments of Neutrophils with Cresyl Violet Philip P. Ostrowski, Ziv Roth, and Sergio Grinstein 16. Neutrophil Degranulation of Azurophil and Specific Granules Samia Bedouhene, Pham My-Chan Dang, Margarita Hurtado-Nedelec, and Jamel El-Benna 17. Influence of Oxygen on Function and Cholesterol Composition of Murine Bone Marrow-Derived Neutrophils Katja Branitzki-Heinemann, Graham Brogden, and Maren von Koeckritz-Blickwede 18. In Vitro Assay for Sensitive Determination of Human Blood PMN Responses Noah Fine, William Khoury, and Michael Glogauer 19. Fast and Accurate Quantitative Analysis of Cytokine Gene Expression in Human Neutrophils by Reverse Transcription Real-Time PCR Nicola Tamassia, Marco A. Cassatella, and Flavia Bazzoni 20. Detection of Intact Transcription Factors in Human Neutrophils Patrick P. McDonald and Richard D. Ye 21. Genome-Scale Transcript Analyses of Human Neutrophils Scott D. Kobayashi, Adeline R. Porter, Sarah L. Anzick, Dan E. Sturdevant, and Frank R. DeLeo Part V: NADPH Oxidase and Production of Reactive Oxygen Species 22. Measurement of Respiratory Burst Products, Released or Retained, During Activation of Professional Phagocytes Claes Dahlgren, Halla Bjoernsdottir, Martina Sundqvist, Karin Christenson, and Johan Bylund 23. Cell-Free NADPH Oxidase Activation Assays: A Triumph of Reductionism Edgar Pick Part VI: Analysis of Neutrophil Extracellular Traps 24. Immunofluorescent Detection of NET Components in Paraffin-Embedded Tissue Ulrike Abu-Abed and Volker Brinkmann 25. Detection, Visualization, and Quantification of Neutrophil Extracellular Traps (NETs) and NET Markers Nicole de Buhr and Maren von Koeckritz-Blickwede 26. Imaging of Neutrophils and Neutrophil Extracellular Traps (Nets) with Intravital (In Vivo) Microscopy Iwona Cichon, Michal Santocki, Weronika Ortmann, and Elzbieta Kolaczkowska

「Nielsen BookData」 より

詳細情報

• NII書誌ID(NCID): BC14113535
• ISBN:
  • 9781071601563
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: New York
• ページ数/冊数: xvi, 470 p.
• 大きさ: 26 cm
• 分類:
  • LCC : QP95.8
  • DC23 : 616.07/9
• 件名:
  • LCSH : Neutrophils -- Laboratory manuals
• 親書誌ID:
  • BA10794673
  • BA88873632