Microtubules : methods and protocols

著者

    • Inaba, Hiroshi

書誌事項

Microtubules : methods and protocols

edited by Hiroshi Inaba

（Methods in molecular biology / John M. Walker, series editor, 2430）（Springer protocols）

Humana Press, c2022

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注記

includes bibliographical references and index

内容説明・目次

内容説明

This volume provides state-of-the-art methodology to functionalize microtubules and construct microtubule-based materials. Chapters consists of six sections detailing modification of microtubule structures, observation and control of microtubule movement, material applications of microtubules in vitro, development of microtubule-binding molecules, comprehensive approaches to analyze properties of microtubules, and functionalization of microtubules in living cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Microtubules: Methods and Protocols aims to provide useful information for construction of microtubule-based materials and help to create new ideas for the next-generation of materials and applications through detailed protocols.

目次

PART I: Preparation and Modification of Microtubules 1. Purification of Tubulin from Porcine Brain and its Fluorescence Dye Modification Satsuki Ishii, Mousumi Akter, Jakia Jannat Keya, Mst. Rubaya Rashid, Farhana Afroze, Syeda Rubaiya Nasrin, and Akira Kakugo 2. Facile Method of Tubulin Purification from Goat Brain for Reconstitution of Microtubule Associated Intracellular Function Satyajit Ghosh, Shubham Garg, Nabanita Mukherjee, and Surajit Ghosh 3. Functionalization of Tubulin: Approaches to Modify Tubulin with Biotin and DNA Mousumi Akter, Jakia Jannat Keya, Arif Md. Rashedul Kabir, Mst. Rubaya Rashid, Satsuki Ishii, and Akira Kakugo 4. Electro-Modulation of Tubulin Properties and Function Djamel Eddine Chafai and Michal Cifra PART II: Observation and Control of Microtubule Movement 5. In Vitro Reconstitution of Microtubule Dynamics and Severing Imaged by Label-Free Interference Reflection Microscopy Yin-wei Kuo and Jonathon Howard 6. Characterizing the Number of Kinesin Motors Bound to Microtubules in the Gliding Motility Assay using FLIC Microscopy Virginia VanDelinder and George D. Bachand 7. Design of Mechanical and Electrical Properties for Multi-Directional Control of Microtubules Hang Zhou, Taikopaul Kaneko, Naoto Isozaki, and Ryuji Yokokawa 8. Linear-Zero Mode Waveguides for Single-Molecule Fluorescence Observation of Nucleotides in Kinesin-Microtubule Motility Assay Kazuya Fujimoto, Ryota Iino, and Ryuji Yokokawa 9. Microtubules and Quantum Dots Integration Leads to Conjugates with Applications in Biosensors and Bionanodevices Xiao Hu and Cerasela Zoica Dinu PART III: Microtubule-Based Active Matters 10. Assembling Microtubule-Based Active Matter Alexandra M. Tayar, Linnea M. Lemma, and Zvonimir Dogic 11. Spontaneous Alignment of Microtubules via Tubulin Polymerization in a Narrow Space under a Temperature Gradient Kazuhiro Shikinaka 12. Dynamic Pattern Formation of Active Matters Triggered by Mechanical Stimuli Jakia Jannat Keya, Arif Md. Rashedul Kabir, Mousumi Akter, and Akira Kakugo 13. Spontaneously Beating Biomimetic Structures Isabella Guido 14. Construction of Molecular Robots from Microtubules for Programmable Swarming Jakia Jannat Keya, Mousumi Akter, Arif Md. Rashedul Kabir, Mst. Rubaya Rashid, and Akira Kakugo 15. Fabrication of Artificial Muscle from Microtubules, Kinesins and DNA Origami Nanostructures Jakia Jannat Keya, Mousumi Akter, Arif Md. Rashedul Kabir, Satsuki Ishi, and Akira Kakugo PART IV: Microtubule-Binding Molecules 16. Encapsulation of Nanomaterials Inside Microtubules by Using a Tau-derived Peptide Hiroshi Inaba and Kazunori Matsuura 17. Investigating Tubulin-Drug Interaction Using Fluorescence Spectroscopy Anuradha Kumari and Dulal Panda PART V: Analysis of Microtubule Dynamics and Structures 18. Visualization and Quantification of Microtubule Self-repair Jeremie Gaillard, Laurent Blanchoin, Manuel Thery, and Laura Schaedel 19. Cargo Transport by Microtubule-Associated Motor Protein Along Mechanically Deformed Microtubules Syeda Rubaiya Nasrin, Arif Md. Rashedul Kabir, and Akira Kakugo 20. Mechanical Deformation of Microtubules on a Two-Dimensional Elastic Medium Syeda Rubaiya Nasrin, Farhana Afroze, Arif Md. Rashedul Kabir, and Akira Kakugo 21. Real-Time Imaging of Single TuRC-Mediated Microtubule Nucleation Events In Vitro by TIRF Microscopy Tanja Consolati, Gil Henkin, Johanna Roostalu, and Thomas Surrey 22. Microtubule Preparation for Investigation with High-Speed Atomic Force Microscopy Christian Ganser and Takayuki Uchihashi 23. Crystallization Systems for the High-Resolution Structural Analysis of Tubulin-Ligand Complexes Tobias Muhlethaler, Natacha Olieric, Valentin A. Ehrhard, Maximilian Wranik, Joerg Standfuss, Ashwani Sharma, Andrea E. Prota, and Michel O. Steinmetz 24. Cryo-EM Visualization of Neuronal Particles Inside Microtubules Sylvie Gory-Faure, Julie Delaroche, Camille Cuveillier, Christian Delphin, and Isabelle Arnal 25. Reconstituting the Interaction Between Purified Nuclei and Microtubule Network Gokce Agsu, Jeremie Gaillard, Bruno Cadot, Laurent Blanchoin, Emmanuelle Fabre, and Manuel Thery PART VI: Modulation of Cellular Microtubules 26. Photocontrolling Microtubule Dynamics with Photoswitchable Chemical Reagents Oliver Thorn-Seshold and Joyce C.M. Meiring 27. Monitoring the Disruptive Effects of Tubulin Binding Agents on Cellular Microtubules Anuradha Kumari and Dulal Panda 28. Pacific Blue-Taxoids as Fluorescent Molecular Probes of Microtubules Angelo E. Andres, Digamber Rane, and Blake R. Peterson 29. Controlling Cell Shape and Microtubule Organization by Extracellular Matrix Micropatterning Alessandro Dema, Shima Rahgozar, Laurent Siquier, Jeffrey van Haren, and Torsten Wittmann

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