Plant transposable elements : methods and protocols

著者

    • Peterson, Thomas

書誌事項

Plant transposable elements : methods and protocols

edited by Thomas Peterson

(Methods in molecular biology / John M. Walker, series editor, 1057)(Springer protocols)

Humana Press, c2013

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

Transposable elements have played a major role in shaping plant genome structure and gene expression. Transposons not only drive sequence expansion, induce mutations and generate chromosome rearrangements, they also help to shape the epigenetic topology of the eukaryotic genome. In Plant Transposable Elements: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study transposons. These methods include computational approaches to study the ancient transposon remnants that comprise the bulk of plant genomes, as well as laboratory techniques to identify recent and ongoing transposition events. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Transposable Elements: Methods and Protocols seeks to aid scientists in the further study of transposons by providing essential background information and specific experimental protocols.

目次

1. Historical Overview of Transposable Element Research Peter A Peterson 2. Distinguishing Variable Phenotypes from Variegation Caused by Transposon Activities Virginia Walbot 3. Using Transposons for Genetic Mosaic Analysis of Plant Development Philip W. Becraft 4. Survey of Natural and Transgenic Gene Markers Used to Monitor Transposon Activity Lakshminarasimhan Krishnaswamy, Thomas Peterson 5. Molecular Biology of Maize Ac/Ds Elements - an Overview Katina Lazarow, My-Linh Du and Reinhard Kunze 6. Gene Tagging with Engineered Ds Elements in Maize Yubin Li, Gregorio Segal, Qinghua Wang, and Hugo K. Dooner 7. Plant Regeneration Methods for Rapid Generation of a Large Scale of Ds Transposant Population in Rice Yuan Hu Xuan, Jin Huang, Gihwan Yi, Dong-Soo Park, Soo Kwon Park, Moo Young Eun, Doh Won Yun, Gang-Seob Lee, Tae Ho Kim, Chang-deok Han 8. Isolation of Sequences Flanking Ac Insertion Sites by Ac Casting Dafang Wang and Thomas Peterson 9. Regulation of the Mutator System of Transposons in Maize Damon Lisch 10. Using MuDR/Mu Transposons in Directed Tagging Strategies Virginia Walbot and Julia Questa 11. Genetic and Molecular Analysis of UniformMu Transposon Insertion Lines Donald R. McCarty, Masaharu Suzuki, Charles Hunter, Wayne T. Avigne, and Karen E. Koch 12. Digestion-Ligation-Amplification (DLA): A Simple Genome Walking Method to Amplify Unknown Sequences Flanking Mutator (Mu) Transposons and Thereby Facilitate Gene Cloning Sanzhen Liu, An-Ping Hsia, Patrick S. Schnable 13. Molecular Genetics and Epigenetics of CACTA Elements Nina V. Fedoroff 14. Activation Tagging Using the Maize En-I Transposon System for the Identification of Abiotic Stress Resistance Genes in Arabidopsis Amal Harb and Andy Pereira 15. Reverse Genetics in Rice using Tos17Delphine Mieulet, Anne Dievart, Gaetan Droc, Nadege Lanau and Emmanuel Guiderdoni 16. Identification and Applications of the Petunia Class II Act1/dTph1 Transposable Element System Tom Gerats , Jan Zethof, and Michiel Vandenbussche 17. Transposon Display: A Versatile Method for Transposon Tagging Michiel Vandenbussche, Jan Zethof, and Tom Gerats 18. Massive Indexed Parallel Identification of Transposon Flanking Sequences Michiel Vandenbussche, Jan Zethof, and Tom Gerats 19. Use of Next Generation Sequencing (NGS) Technologies for the Genome-wide Detection of Transposition Moaine Elbaidouri, Cristian Chaparro and Olivier Panaud 20. Overview of Repeat Annotation and de novo Repeat Identification Ning Jiang 21. Computational Methods for Identification of DNA Transposons Ning Jiang 22. TEnest 2.0: Computational Annotation and Visualization of Nested Transposable Elements Brent A. Kronmiller and Roger P. Wise

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詳細情報

  • NII書誌ID(NCID)
    BC14850599
  • ISBN
    • 9781627035675
  • LCCN
    2013942868
  • 出版国コード
    us
  • タイトル言語コード
    eng
  • 本文言語コード
    eng
  • 出版地
    New York
  • ページ数/冊数
    xv, 324 p.
  • 大きさ
    26 cm
  • 親書誌ID
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